BackgroundOA it's a common disease that occur in elderly people and it its most come in the female under age 49 than a male above 50 because of the hormonal changes and for the men for aging and other chronic disease. ObjectiveOur objective was directed to determine the beneficial effect of the antioxidants (vitamin E and C) as anti-inflammatory in OA patients treated with diclofenac sodium.There is a reality that oxidative stress give a good progression and incidence of osteoarthritis (OA), increased production of reactive oxygen species (ROS) and decreased bioavailability of antioxidants have been checked by experimental and human OA, where increased ROS production may contribute to the elevation in OA incidence. and we use in this study different ages with OA patient to can determind the best reslu and effect we can get from this study. Patients and methodsThe ESR, Hgb, and serum (CRP, RF, MDA, and GSH) level were measured for each patient before treatment (at the baseline) and after 3, 5, 7, and 8 weeks of the treatment with documentation of symptoms and pain score before and after treatment (the treatment were continue for 2 months).ResultsThere was significant (p 0.05) elevation in serum MDA level and significant reduction in serum GSH level in OA patients as compared to the healthy control subjects. The addition of vitamin E to diclofenac sodium (group B) had resulted in a significant lowering in serum MDA level with significant elevation in serum GSH level and OA outcome score (symptoms and signs score) after 3, 5, 7, and 8 weeks of treatment, likewise the addition of vitamin C to diclofenac sodium (group C) with less positive effect than vitamin E, while the use of combined antioxidants vitamin E and C (group D) resulted in significant lowering in serum MDA level with significant elevation in serum GSH level and OA outcome score (symptoms and pain score) after 3, 5, 7, and 8 weeks of the treatment with great positive effect than the use of single antioxidant (vitamin E or C) due to synergistic effect.ConclusionThe oxidative stress give a progression of OA and the addition of antioxidants (vitamin E or C) to diclofenac sodium treatment give a good lowering in MDA level, and elevation in GSH level and OA outcome score (symptoms and pain score) with improving the patients compliance but all the result that I got it gave a negative result with African races . Note.that test and that laboratory data I got it from the patient directly or from their physician after I get from them official agreement that was a proved by their physician so I got a full agreement to get a full excess for their laboratory data.