In this study vine leaf (Vitis vinifera) (VL) was used. For the dying bath the appropriate solventphase;, mordant and pH degrees were found, and for the hypothetical testing such as dye quantity,dying duration, the sunshine exposure time and the light source were determined depending on theheuristic results. Via these determination onion stem cells (OSC) and stimulated human tlymphocytechromosome preparations (CP) were dyed.In dye bath as solvent phase distiller water (DW) + methyl alcohol, DW +ethyl alcohol, DW+acetic acid, DW+ sodium hydroxide; as mordant materials ferrous-2-sulfate (FeSO4), alum, alum+glycine, alum + L-sistein and alum + potassium bichromate (K2Cr2O7) were tested seperately. AspH degrees 1,2,3,4,5,8 and 11 ; as the light sources sun light and ultraviolet (UV) (30 minutes in280 nm wave light) ; 1,5 ; 2,0 ; 2,5 hours of dyeing time; 15, 30, 45, 60 minutes of exposure to sunlight were tested.When the dye bath was adjusted to pH=4, as liquid phase;, distiller water + acetic acid; alummordant and sun light were used, the dying was achieved and the best image was attained. Inconclusion, the value of vine leaf extract (VLE) as a potential fluorescence dye has beendemonstrated. Other tested dye baths (DW+ methyl alcohol, , DW +ethyl alcohol, DW+ sodiumhydroxide) , mordants (FeSO4, alum +glycine, alum + L-sistein, alum+ K2Cr2O7), pH degrees (1,2,3,5,8,11) and in UV light source an observable dye in light or fluoresance microscope could notbe obtained.

Bu çalısmada, boya kaynagı olarak asma yapragı (Vitis vinifera) (AY) kullanılmıstır. Boya banyosuolarak uygun; çözücü fazı, mordan ve pH degerini bulmak kosuluyla, boya miktarı, boyama süresi,günese tutma süresi ve ısık kaynagı gibi parametreleri deneme yanılma yoluyla belirleyerek, sogankök hücreleri (SKH) ve stimule edilmis insan t-lenfosit kromozom yayma preparatları (KYP)boyanmıstır.Boya banyosunda; çözücü olarak, distile su (DS) + metil alkol, DS + etil alkol, DS + asetik asit, DS+ sodyum hidroksit; mordan maddeleri olarak demir-2-sülfat (FeSO4), sap, sap + glisin, sap + Lsisteinve sap + potasyum bikromat (K2Cr2O7) tek tek denenmistir. pH degerleri olarak 1,2,3,4,5,8ve 11 ; ısık kaynagı olarak günes ısıgı ve ultraviyole (UV) (280 nm dalga boyunda 30 dk); 1,5 ; 2,0 ;2,5 saatlik kaynatma süreleri ; 15, 30, 45, 60 dakikalık günes ısıgına tutma süreleri ayrı ayrıdenenmistir.Boya banyosu pH=4' e ayarlandıgında, sıvı fazı olarak distile su+asetik asit, mordan maddesi olaraksap ve ısık kaynagı olarak günes ısıgı kullanıldıgında boyanma gerçeklesmis ve en iyi görüntü eldeedilmistir. Sonuç olarak asma yapragı özütü (AYÖ)' nün potansiyel bir floresan boya degeri oldugukararı verilmistir. Diger denenen boya banyoları (DS + metil alkol, DS + etil alkol, DS + sodyumhidroksit), mordanlar (FeSO4, sap + glisin, sap + L-sistein, sap + K2Cr2O7), pH degerleri (1,2,3,5,8,11) ve UV ısık kaynagında, ısık yada floresan mikroskobunda gözlemlenebilir birboyanma saglanamamıstır.