Lung cancer is the leading cause of cancer mortality in theworld, accounting for %16 of all cancers and %28 of all cancer deaths (35%men, 19% women). There are several environmental and genetic factorswhich could be changed in each individual, effect lung cancer risk. Thegenomic alterations can exist in molecular pathways, such as regulation ofcell cycle.The Aurora-A kinase which is a member of protein kinase family,regulates the functions of centrosomes, spindles and kinetochores requiredfor proper mitotic progression. The kinase function can change with the F31Iand V57I polymorphisms in Aurora-A gene. Our aim is to reveal thepolymorphic variations of codon 31 and 57 in Turkish population and find outthe relation with lung cancer.Included in this study, 86 incident lung cancer cases who arenewly diagnosed and 102 age frequency-matched, non smoking, no familialcancer history, randomly selected community controls were studied todetermine Aurora-A polymorphisms. The genomic DNA was extracted fromwhole blood. After the CR reaction all samples were genotyped with DNAsequence technique.The frequencies of both genotypes (he31 lie, Val57lle) in lungcancer patients were not significantly different from those in controls.earson's x2 p values were found 0,430 and 0,73, respectively (p>0.05).The present study is the first Turkish data which reveals therelation between Aurora-A polymorphisms and lung cancer risk. The resultsof our and many other studies contribute to the efforts to understand theassociation between lung cancer and Aurora-A polymorphisms as well as tothe prospective diagnosis and treatment phases.Keywords: Aurora-A, polymorphism, lung cancer, sequence.

Turn kanserlerin %16'si, turn kanser olumlerinin %28'i(erkeklerde %35, kadinlarda %19) akciger kanseri nedeniyledir. Akcigerkanser riskini etkileyen bir?ok gevresel faktor ve bireysel genomik degisimlerbulunmaktadir. Bu degisimler, hucre dongusunun kontrolu gibi gesitlimolekuler yolaklarda olabilmektedir.rotein kinaz ailesinin bir uyesi olan Aurora kinazlar hucrebolunmesi sirasinda, kromozom aynlmasinda, ig ipliklerinin biraradabulunmasi ve duzenliliginde ve kromozomlann yogunlasmasinda rol ahrlar.Aurora-A'nin kinaz islevi, gende bulunan F31I ve V57I polimorfizmleriyledegi§ebilmektedir. Bizim amacimiz Turk toplumunda bu genin kodon 31 ve57'deki polimorfik degisimlerini ve akciger kanseri ile iliskisini ortayaQikarmaktir.Akciger kanserli hastalarda Aurora-A genindeki polimorfizmleribelirlemek amaciyla gahsmamiza yeni tani aim is 86 akciger kanser hastasive ailesinde kanser oykusu bulunmayan, sigara igmeyen ve yas arahgihastalarla uyumlu, saghkh 102 birey dahil edilmi§tir. Tarn kandan genomikDNA izolasyonun ardindan, PCR yapilmis ve turn ornekler DNA dizilimteknigiyle genotiplendirilmi§tir.Sonuglanmiza gore akciger kanserli hasta ve kontrol grubu,F31I ve V57I polimorfizmleri bakimindan karsilastinldiginda risk faktoruolu§turabilecek bir ili§ki bulunmami§tir. earson's x2 p degerleri sirasiyla;0,430 ve 0, 473'dur ( p>0.05).Kanser geli§iminde etkili rol oynayan genlerden biri olan Aurora-A'nin polimorfizmlerinin, akciger kanseriyle iliskisini ortaya koyan buarastirma, toplumumuzda yapilan ilk ?ahsma ozelligini ta§imaktadir.Qahsmamizin ve bu konuda yapilan diger ara§tirmalann sonuglan, Aurora-Apolimorfizmleriyle akciger kanseri arasindaki iliskinin daha iyi anlasilmasina,gelecekte kanser tanisi ve tedavisine yeni yaklasimlar getirilebilmesine katkisaglayabilecektir.Anahtar kelimeler: Aurora-A, polimorfizm, akciger kanseri, dizi analizi.