The Antiproliferative Activity of Colchicum umbrosum Plant Extract and Paclitaxel on C-4 I and Vero Cells

Ozlem DAGDEVİREN OZSOYLEMEZ; Gul OZCAN

Makale detayları

The Antiproliferative Activity of Colchicum umbrosum Plant Extract and Paclitaxel on C-4 I and Vero Cells

Yazarlar:

Ozlem DAGDEVİREN OZSOYLEMEZ | Gul OZCAN

Yayıncı:

Istanbul University

Dergi:

European Journal of Biology 2018 Vol.77 Issue 2, pp.81-88

Belge Tipi:

Makale

Yayın Tarihi:

12/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000424466

eISSN:

2618-6144

pISSN:

2602-2575

Özet:

 


DOI: 10.26650/EurJBiol.2018.0016


Objective: This study was conducted to
determine the antiproliferative effects of paclitaxel (PAC) and Colchicum
umbrosum plant extract on C-4 I and Vero cells. In addition, the apoptotic
effects of plant extract on these cells were compared.

Materials and Methods: C-4 I and Vero cells
were treated with PAC and corm extract of C. umbrosum for 24 h. The
antiproliferative effects of 0.01, 0.1, and 1 mg/mL of plant extract and 7.5,
15, and 30 nM of PAC and the half maximal inhibitory concentration (IC50)
values were determined by the MTT method. Morphological changes occurring in
the C-4 I and Vero cells were observed under phase-contrast and fluorescence
microscopes using DAPI staining.

Results: The IC50 values were found to be
0.01 mg/mL in C-4 I cells and 1 mg/mL in Vero cells for the plant extract and
15 nM in C-4 I and Vero cells for PAC. The apoptotic index (AI) values of the
experimental groups of C-4 I and Vero cells were significantly increased
compared to those of the control group (p<0.01) after treatment with IC50
concentration of the plant extract. The AI values of the plant extract
treatment in C-4 I cells were higher than those in Vero cells at 24 and 48 h.

Conclusion: Treatment with the IC50
concentration of the plant extract induced apoptotic cell death in C-4 I cells.
It can worth be considered as a novel promising candidate for the treatment of
cancer.

.

Makale detayları

The Antiproliferative Activity of Colchicum umbrosum Plant Extract and Paclitaxel on C-4 I and Vero Cells

Yazarlar: Ozlem DAGDEVİREN OZSOYLEMEZ | Gul OZCAN

Yayıncı: Istanbul University

Dergi: European Journal of Biology 2018 Vol.77 Issue 2, pp.81-88

Belge Tipi: Makale

Yayın Tarihi: 12/2018

Belge Dili: İngilizce

Document ID (Org No.): 2000424466

eISSN: 2618-6144

pISSN: 2602-2575

Sayı

Yazarlar:

Ozlem DAGDEVİREN OZSOYLEMEZ | Gul OZCAN

Yayıncı:

Istanbul University

Dergi:

European Journal of Biology 2018 Vol.77 Issue 2, pp.81-88

Belge Tipi:

Makale

Yayın Tarihi:

12/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000424466

eISSN:

2618-6144

pISSN:

2602-2575