The expression levels of MIR375 and MIR451a genes in subacute thyroiditis

Mustafa Koçak; Nevin Karakuş; Merve Çatak

Makale detayları

The expression levels of MIR375 and MIR451a genes in subacute thyroiditis

Yazarlar:

Mustafa Koçak | Nevin Karakuş | Merve Çatak

Yayıncı:

Cukurova University

Ana Başlıklar:

Tıbbi Genetik (Kanser Genetiği hariç)

Dergi:

Cukurova Medical Journal Vol.49 Issue 2, pp.260-267

Belge Tipi:

Makale

Yayın Tarihi:

6/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001320777

pISSN:

2602-3032

Anahtar kelimeler:

Özet:

 Purpose: The most typical reason for thyroid pain is subacute thyroiditis SAT. Incidence of SAT is higher in women than in men and the average annual frequency of SAT is 12.1 cases per 100,000 people. Endogenous, brief, single-stranded non-coding RNAs, called micro RNAs miRNAs, are inhibitors of gene expression. In the posttranscriptional regulation of gene expression, miRNAs are essential. In this investigation, we explored the potential utility of two circulating miRNAs, MIR451A and MIR375, as markers for the detection and monitoring of SAT. Materials and Methods: Fifty SAT patients and 41 healthy people were enrolled in this study. Using the quantitative real-time polymerase chain reaction qRT-PCR approach, miRNA expression levels were assessed. The 2-ΔΔCt technique was used to calculate miRNA expression levels. The t-test was used to assess the statistical significance of miRNA expression. Results: While MIR451A expression levels were discovered to vary between healthy controls and SAT patients, MIR375-3p expression levels were discovered to be similar in both groups. MIR375-3p and MIR451A expression levels were shown to be similarly in different SAT stages hyperthyroid, euthyroid, and hypothyroid. The expression levels of MIR375-3p and MIR451A were analyzed for association with the clinical characteristics of SAT patients, but no correlation was observed. Conclusion: In this study, it was found that the expression level of circulating MIR451A was lower in SAT patients, which shows that MIR451A might be effective in the SAT diseases onset.

 Purpose: The most typical reason for thyroid pain is subacute thyroiditis SAT. Incidence of SAT is higher in women than in men and the average annual frequency of SAT is 12.1 cases per 100,000 people. Endogenous, brief, single-stranded non-coding RNAs, called micro RNAs miRNAs, are inhibitors of gene expression. In the posttranscriptional regulation of gene expression, miRNAs are essential. In this investigation, we explored the potential utility of two circulating miRNAs, MIR451A and MIR375, as markers for the detection and monitoring of SAT. Materials and Methods: Fifty SAT patients and 41 healthy people were enrolled in this study. Using the quantitative real-time polymerase chain reaction qRT-PCR approach, miRNA expression levels were assessed. The 2-ΔΔCt technique was used to calculate miRNA expression levels. The t-test was used to assess the statistical significance of miRNA expression. Results: While MIR451A expression levels were discovered to vary between healthy controls and SAT patients, MIR375-3p expression levels were discovered to be similar in both groups. MIR375-3p and MIR451A expression levels were shown to be similarly in different SAT stages hyperthyroid, euthyroid, and hypothyroid. The expression levels of MIR375-3p and MIR451A were analyzed for association with the clinical characteristics of SAT patients, but no correlation was observed. Conclusion: In this study, it was found that the expression level of circulating MIR451A was lower in SAT patients, which shows that MIR451A might be effective in the SAT diseases onset.

 Purpose: The most typical reason for thyroid pain is subacute thyroiditis SAT. Incidence of SAT is higher in women than in men and the average annual frequency of SAT is 12.1 cases per 100,000 people. Endogenous, brief, single-stranded non-coding RNAs, called micro RNAs miRNAs, are inhibitors of gene expression. In the posttranscriptional regulation of gene expression, miRNAs are essential. In this investigation, we explored the potential utility of two circulating miRNAs, MIR451A and MIR375, as markers for the detection and monitoring of SAT. Materials and Methods: Fifty SAT patients and 41 healthy people were enrolled in this study. Using the quantitative real-time polymerase chain reaction qRT-PCR approach, miRNA expression levels were assessed. The 2-ΔΔCt technique was used to calculate miRNA expression levels. The t-test was used to assess the statistical significance of miRNA expression. Results: While MIR451A expression levels were discovered to vary between healthy controls and SAT patients, MIR375-3p expression levels were discovered to be similar in both groups. MIR375-3p and MIR451A expression levels were shown to be similarly in different SAT stages hyperthyroid, euthyroid, and hypothyroid. The expression levels of MIR375-3p and MIR451A were analyzed for association with the clinical characteristics of SAT patients, but no correlation was observed. Conclusion: In this study, it was found that the expression level of circulating MIR451A was lower in SAT patients, which shows that MIR451A might be effective in the SAT diseases onset.

 Amaç: Tiroid ağrısının en tipik nedeni subakut tiroidittir SAT. SAT prevalansı kadınlarda erkeklere göre daha yüksektir ve yıllık ortalama sıklığı 100.000 kişide 12,1dir. Mikro RNAlar miRNAlar adı verilen endojen, kısa, tek sarmallı, kodlamayan RNAlar, gen ekspresyonunun inhibitörleridir. miRNAlar gen ifadesinin transkripsiyon sonrası düzenlenmesinde önemlidir. Bu çalışmada, dolaşımdaki iki miRNAnın MIR451A ve MIR375 SATın tespiti ve izlenmesinde belirteç olarak potansiyel faydasını araştırdık. Gereç ve Yöntem: Bu çalışmaya 50 SAT hastası ve 41 sağlıklı birey dahil edildi. miRNA ekspresyon seviyeleri, kantitatif gerçek zamanlı polimeraz zincir reaksiyonu qRT-PCR yaklaşımı kullanılarak değerlendirildi. miRNA ifade düzeylerinin hesaplamak için 2-ΔΔCt tekniği kullanıldı. miRNA ifadesinin istatistiksel önemini değerlendirmek için t testi kullanıldı. Bulgular: Sağlıklı kontroller ve SAT hastaları arasında MIR451A ekspresyon düzeyleri farklılık gösterirken, MIR375-3p ekspresyon düzeylerinin iki grupta da benzer olduğu belirlendi. MIR375-3p ve MIR451A ekspresyon düzeylerinin, farklı SAT evrelerinde hipertiroid, ötiroid ve hipotiroid benzer olduğu görüldü. MIR375-3p ve MIR451Anın ekspresyon seviyeleri, SAT hastalarının klinik özellikleriyle ilişki açısından analiz edildi, ancak herhangi bir korelasyona rastlanmadı. Sonuç: Bu çalışmada dolaşımdaki MIR451Anın ekspresyon düzeyinin SAT hastalarında daha düşük olduğu bulundu, bu da MIR451Anın SAT hastalığının başlangıcında etkili olabileceğini göstermektedir.

Makale detayları

The expression levels of MIR375 and MIR451a genes in subacute thyroiditis

Yazarlar:

Mustafa Koçak | Nevin Karakuş | Merve Çatak

Yayıncı:

Cukurova University

Ana Başlıklar:

Tıbbi Genetik (Kanser Genetiği hariç)

Dergi:

Cukurova Medical Journal Vol.49 Issue 2, pp.260-267

Belge Tipi:

Makale

Yayın Tarihi:

6/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001320777

pISSN:

2602-3032

expression level

subacute thyroiditis

MIR451A

MIR375ekspresyon düzeyi

subakut tiroidit

MIR451A

MIR375.

Sayı

Makale detayları

Yazarlar: Mustafa Koçak | Nevin Karakuş | Merve Çatak

Yayıncı: Cukurova University

Ana Başlıklar: Tıbbi Genetik (Kanser Genetiği hariç)

Dergi: Cukurova Medical Journal Vol.49 Issue 2, pp.260-267

Belge Tipi: Makale

Yayın Tarihi: 6/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001320777

pISSN: 2602-3032

Sayı

Yazarlar:

Mustafa Koçak | Nevin Karakuş | Merve Çatak

Yayıncı:

Cukurova University

Ana Başlıklar:

Tıbbi Genetik (Kanser Genetiği hariç)

Dergi:

Cukurova Medical Journal Vol.49 Issue 2, pp.260-267

Belge Tipi:

Makale

Yayın Tarihi:

6/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001320777

pISSN:

2602-3032