Determination of Clopidogrel in Pharmaceutical Preparation by UV Spectrophotometry and High Performance Liquid Chromatography Methods

Ömer Faruk KOÇAK; Yücel KADIOĞLU; Onur ŞENOL

Makale detayları

Determination of Clopidogrel in Pharmaceutical Preparation by UV Spectrophotometry and High Performance Liquid Chromatography Methods

Yazarlar:

Ömer Faruk KOÇAK | Yücel KADIOĞLU | Onur ŞENOL

Yayıncı:

Muhammet Kaan YEŞİLYURT

Ana Başlıklar:

Uygulamalı fizik

Dergi:

International Journal of Innovative Research and Reviews 2020 Vol.4 Issue 1, pp.14-19

Belge Tipi:

Makale

Yayın Tarihi:

7/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000466531

Sıklık:

İki Yıllık

eISSN:

2636-8919

Özet:

 

UV-Visible  spectroscopy  and  high  performance  liquid  chromatography  (HPLC)  methods were developed and validated for determination of clopidogrel in bulk and pharmaceutical formulations. In spectroscopy, clopidogrel spectrum at different solutions was taken and the best  absorbance  value   was  obtained  at  202  nm   wavelength   in   methanol-acetonitrile (50:50.h/h)  mixture.  In  HPLC,  parameters  were  optimized  as  follows:  mobile  phase  was acetonitrile-methanol-water  (45:45:10,  v/v/v),  C18  re-serve  phase  column  was  preferred, flow  rate  and  injection  volume  were  set  as  0.9  mL/min  and  10  μL,  respectively.  All measurements  were  performed at  230 nm  wavelength. Meloxicam  was  used as an internal standard in HPLC. Linearity, analytic recovery, intra and inter-days precision and accuracy were  investigated  in  order  to  per-form  validation  process,  It  was  stated  that  HPLC measurements  were  linear  at  0.25-30  μg/mL  while  UV-Visible  method  linear  at  1.25-25 μg/mL. Intra and inter-days precision and accuracy values were 5.38% and 2.66% for HPLC method and 3.77% and 3.60% for UV-Vis. Mean analytic recovery  value  was found to  be 99.5% for both methods. Developed and validated HPLC and UV-Vis methods were applied to pharmaceutical preparations including clopidogrel.

Makale detayları

Determination of Clopidogrel in Pharmaceutical Preparation by UV Spectrophotometry and High Performance Liquid Chromatography Methods

Yazarlar: Ömer Faruk KOÇAK | Yücel KADIOĞLU | Onur ŞENOL

Yayıncı: Muhammet Kaan YEŞİLYURT

Ana Başlıklar: Uygulamalı fizik

Dergi: International Journal of Innovative Research and Reviews 2020 Vol.4 Issue 1, pp.14-19

Belge Tipi: Makale

Yayın Tarihi: 7/2020

Belge Dili: İngilizce

Document ID (Org No.): 2000466531

Sıklık: İki Yıllık

eISSN: 2636-8919

Sayı

Yazarlar:

Ömer Faruk KOÇAK | Yücel KADIOĞLU | Onur ŞENOL

Yayıncı:

Muhammet Kaan YEŞİLYURT

Ana Başlıklar:

Uygulamalı fizik

Dergi:

International Journal of Innovative Research and Reviews 2020 Vol.4 Issue 1, pp.14-19

Belge Tipi:

Makale

Yayın Tarihi:

7/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000466531

Sıklık:

İki Yıllık

eISSN:

2636-8919