ANTIPROLIFERATIVE EFFECTS OF VITAMIN K2 IN OSTEOSARCOMA CELLS: COMPARISON OF DIFFERENT CYTOTOXICITY ANALYZES

Büşranur Özalper; Tuba Özdemir Sancı; Habibe Özgüner

Makale detayları

ANTIPROLIFERATIVE EFFECTS OF VITAMIN K2 IN OSTEOSARCOMA CELLS: COMPARISON OF DIFFERENT CYTOTOXICITY ANALYZES

Yazarlar:

Büşranur Özalper | Tuba Özdemir Sancı | Habibe Özgüner

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Clinical Sciences

Dergi:

Medical Journal of Süleyman Demirel University Vol.30 Issue 1, pp.1-8

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001285074

pISSN:

1300-7416

Anahtar kelimeler:

Özet:

 Objective The aim of this study was to compare four commonly used cytotoxicity assays XTT, neutral red uptake, crys- tal violet assay, and propidium iodide staining which analyzed the antiproliferative effects of vitamin K2. Material and Method Saos-2 cells, an osteosarcoma cell line, were exposed to vitamin K2 at different concentrations 10 μM, 20 μM, 30 μM, 40 μM, 50 μM, 100 μM and 200 μM for 48 and 72 hours. Tetrazolium salt test XTT, neutral red uptake NR assay, crystal violet assay, and propidium iodide PI staining were performed to determine cytotoxic potential of vitamin K2 in terms of the cell viability and IC50 values. The results were evaluated with one-way analysis of variance ANOVA and the Tukey test. Results Cytotoxic effects of vitamin K2 on osteosarcoma cells were analyzed with XTT, neutral red, crystal violet assay, and propidium iodide, respectively. IC50 values were determined exposure to 61.93; 40.21; 62.11; 70.57 μM vitamin K2 for 48 and 75.44; 68.22; 41.66; 88.01 μM vitamin K2 for 72 hours. Conclusion Statistical analysis revealed that there is a significant statistical difference between four tests used in this study. In addition, it was determined that the viability rates in propidium iodide staining were higher than other tests for cytotoxicity analyses. It has been concluded that incubations at different concentrations were required to prevent misinterpretation of results in cytotoxicity analyses, and tetrazolium salt-based tests should be supplemented with other non-metabolic tests.

 Objective The aim of this study was to compare four commonly used cytotoxicity assays XTT, neutral red uptake, crys- tal violet assay, and propidium iodide staining which analyzed the antiproliferative effects of vitamin K2. Material and Method Saos-2 cells, an osteosarcoma cell line, were exposed to vitamin K2 at different concentrations 10 μM, 20 μM, 30 μM, 40 μM, 50 μM, 100 μM and 200 μM for 48 and 72 hours. Tetrazolium salt test XTT, neutral red uptake NR assay, crystal violet assay, and propidium iodide PI staining were performed to determine cytotoxic potential of vitamin K2 in terms of the cell viability and IC50 values. The results were evaluated with one-way analysis of variance ANOVA and the Tukey test. Results Cytotoxic effects of vitamin K2 on osteosarcoma cells were analyzed with XTT, neutral red, crystal violet assay, and propidium iodide, respectively. IC50 values were determined exposure to 61.93; 40.21; 62.11; 70.57 μM vitamin K2 for 48 and 75.44; 68.22; 41.66; 88.01 μM vitamin K2 for 72 hours. Conclusion Statistical analysis revealed that there is a significant statistical difference between four tests used in this study. In addition, it was determined that the viability rates in propidium iodide staining were higher than other tests for cytotoxicity analyses. It has been concluded that incubations at different concentrations were required to prevent misinterpretation of results in cytotoxicity analyses, and tetrazolium salt-based tests should be supplemented with other non-metabolic tests.

 Objective The aim of this study was to compare four commonly used cytotoxicity assays XTT, neutral red uptake, crys- tal violet assay, and propidium iodide staining which analyzed the antiproliferative effects of vitamin K2. Material and Method Saos-2 cells, an osteosarcoma cell line, were exposed to vitamin K2 at different concentrations 10 μM, 20 μM, 30 μM, 40 μM, 50 μM, 100 μM and 200 μM for 48 and 72 hours. Tetrazolium salt test XTT, neutral red uptake NR assay, crystal violet assay, and propidium iodide PI staining were performed to determine cytotoxic potential of vitamin K2 in terms of the cell viability and IC50 values. The results were evaluated with one-way analysis of variance ANOVA and the Tukey test. Results Cytotoxic effects of vitamin K2 on osteosarcoma cells were analyzed with XTT, neutral red, crystal violet assay, and propidium iodide, respectively. IC50 values were determined exposure to 61.93; 40.21; 62.11; 70.57 μM vitamin K2 for 48 and 75.44; 68.22; 41.66; 88.01 μM vitamin K2 for 72 hours. Conclusion Statistical analysis revealed that there is a significant statistical difference between four tests used in this study. In addition, it was determined that the viability rates in propidium iodide staining were higher than other tests for cytotoxicity analyses. It has been concluded that incubations at different concentrations were required to prevent misinterpretation of results in cytotoxicity analyses, and tetrazolium salt-based tests should be supplemented with other non-metabolic tests.

 Amaç Bu çalışmanın amacı, K2 vitamininin antiproliferatif etkilerini saptamak için yaygın olarak kullanılan dört sitotoksisite analizinin XTT, nötral kırmızı alım, kristal viyole ve propidyum iyodür boyama doğruluk potansiyelini karşılaştırmaktır. Gereç ve Yöntem Osteosarkoma hücre hattı olan Saos-2 hücreleri, 48 ve 72 saat boyunca K2 vitaminine 10 μM, 20 μM, 30 μM, 40 μM, 50 μM, 100 μM ve 200 μM maruz bırakıldı. Tetrazolyum tuzu testi XTT, nötral kırmızı alım testi NR, kristal viyole testi ve propidyum iyodür PI boyama yapılarak hücrelerin canlılık oranlarına göre K2 vitamini sitotoksisitesi belirlendi ve farklı dozların IC50 değerleri hesaplandı. Analiz sonuçları tek yönlü varyans analizi ANOVA ve Tukey testiyle değerlendirildi. Bulgular K2 vitamininin osteosarkoma hücreleri üzerinde sitotoksik etkileri olduğu sırasıyla XTT, NR, kristal viyole testi ve PI yöntemleri kullanılarak belirlendi ve IC50 değerleri 48 saatlik maruziyet için sırasıyla 61,93; 40,21; 62,11; 70,57 μM ve 72 saatlik maruziyet için sırasıyla 75,44; 68,22; 41,66; 88,01 μM olarak saptandı. Sonuç İstatistiksel analizler çalışmada uygulanan testlerden elde edilen sonuçların farklılık gösterdiğini ortaya koymuştur. Ayrıca propidyum iyodür boyama sonucundaki canlılık oranlarının diğer testlerle elde edilen sonuçlara kıyasla çarpıcı bir şekilde daha yüksek olduğu belirlenmiştir. Sitotoksisite analizlerinde sonuçların yanlış yorumlanmasını önlemek için farklı zamanlarda ve farklı konsantrasyonlarda inkübasyonlar gerektiği ve ayrıca tetrazolyum tuzu temelli testlerin metabolik olmayan diğer testlerle desteklenmesi gerektiği sonucuna varılmıştır.

Makale detayları

ANTIPROLIFERATIVE EFFECTS OF VITAMIN K2 IN OSTEOSARCOMA CELLS: COMPARISON OF DIFFERENT CYTOTOXICITY ANALYZES

Yazarlar:

Büşranur Özalper | Tuba Özdemir Sancı | Habibe Özgüner

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Clinical Sciences

Dergi:

Medical Journal of Süleyman Demirel University Vol.30 Issue 1, pp.1-8

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001285074

pISSN:

1300-7416

In vitro cytotoxicity

Cell viability tests

Osteosarcoma

Vitamin K.

İn vitro sitotoksisite

Hücre canlılık testleri

Osteosarkoma

K vitamini.

Sayı

Makale detayları

Yazarlar: Büşranur Özalper | Tuba Özdemir Sancı | Habibe Özgüner

Yayıncı: Süleyman Demirel University

Ana Başlıklar: Clinical Sciences

Dergi: Medical Journal of Süleyman Demirel University Vol.30 Issue 1, pp.1-8

Belge Tipi: Makale

Yayın Tarihi: 3/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001285074

pISSN: 1300-7416

Sayı

Yazarlar:

Büşranur Özalper | Tuba Özdemir Sancı | Habibe Özgüner

Yayıncı:

Süleyman Demirel University

Ana Başlıklar:

Clinical Sciences

Dergi:

Medical Journal of Süleyman Demirel University Vol.30 Issue 1, pp.1-8

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001285074

pISSN:

1300-7416