Amylase in the hepatopancreas of a euryhaline burrowing  crab: characteristics and modulation

ANTONELA ASARO; ROBERTO PAGGI; ROSANA DE CASTRO; ALEJANDRA LOPEZ MAÑANES

Makale detayları

Amylase in the hepatopancreas of a euryhaline burrowing crab: characteristics and modulation

Yazarlar:

ANTONELA ASARO | ROBERTO PAGGI | ROSANA DE CASTRO | ALEJANDRA LOPEZ MAÑANES

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Zoology 2017 Vol.41 Issue 3, pp.443-453

Belge Tipi:

Makale

Yayın Tarihi:

5/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000774046

Sıklık:

İki Aylık

eISSN:

1303-6114

pISSN:

1300-0179

Özet:

 

In spite of its inherent physiological importance, studies on the occurrence, characteristics, and modulation of amylase in euryhaline crabs are lacking. We investigated the occurrence of amylase forms and the effect of acclimation to different salinities on their number and made a partial purification and characterization of the major form present in the hepatopancreas of Neohelice granulata. Zymogram analysis revealed 5 amylase forms in crabs acclimated to 35 psu (seawater) and 37 psu, and an additional band at 10 psu, but with a major form (29 kDa) in all cases, which was partially purified and characterized. Amylolytic activity was maximal between 30 and 40 °C; maintained at high NaCl concentrations (up to 4 M); increased by 5 mM K+, Li+, Co2+, and Mg2+ (36%-45%); inhibited by Cu2+, Zn2+, Cd2+, Fe2+, and Mn2+ (92.4% and 23.7%); not affected by Ni2+ or Ba2+; and enhanced almost 100% by Ca2+. Amylase exhibited Michaelis-Menten kinetics (starch: Km = 1.24 mg mL-1; glycogen: Km = 16.19 mg mL-1). The potential physiological significance and relationship to habitat conditions of the extra form in low salinity and the biochemical characteristics of the partially purified amylolytic activity (halotolerant, differential sensitivity to ions, capability to hydrolyze starch and glycogen) are discussed.

Makale detayları

Amylase in the hepatopancreas of a euryhaline burrowing crab: characteristics and modulation

Yazarlar: ANTONELA ASARO | ROBERTO PAGGI | ROSANA DE CASTRO | ALEJANDRA LOPEZ MAÑANES

Yayıncı: TUBITAK

Dergi: Turkish Journal of Zoology 2017 Vol.41 Issue 3, pp.443-453

Belge Tipi: Makale

Yayın Tarihi: 5/2017

Belge Dili: Türkçe

Document ID (Org No.): 2000774046

Sıklık: İki Aylık

eISSN: 1303-6114

pISSN: 1300-0179

Sayı

Yazarlar:

ANTONELA ASARO | ROBERTO PAGGI | ROSANA DE CASTRO | ALEJANDRA LOPEZ MAÑANES

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Zoology 2017 Vol.41 Issue 3, pp.443-453

Belge Tipi:

Makale

Yayın Tarihi:

5/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000774046

Sıklık:

İki Aylık

eISSN:

1303-6114

pISSN:

1300-0179