Simultaneous Quantification of Multi-Class Antimicrobials in Chicken Kidney and Liver by New Validated UPLC-MS/MS Method

Sathish Kumar Konidala; Shridhar Narayanan

Makale detayları

Simultaneous Quantification of Multi-Class Antimicrobials in Chicken Kidney and Liver by New Validated UPLC-MS/MS Method

Yazarlar:

Sathish Kumar Konidala | Shridhar Narayanan

Yayıncı:

Hacettepe University

Ana Başlıklar:

Pharmaceutical Analytical Chemistry

Dergi:

Hacettepe University Journal of the Faculty of Pharmacy Vol.45 Issue 1, pp.30-42

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001341854

pISSN:

2458-8806

Anahtar kelimeler:

Özet:

 A novel and significant method was developed and validated with a sensitive, rapid, and simultaneous analytical method to determine antimicrobials in chicken tissues such as the kidney and liver. The process involved a unique approach to precipitation extraction. This method has not been widely used in this context, followed by the evaporation of the supernatant and reconstitution with the mobile phase. Antimicrobials, including Azithromycin, Clarithromycin, Erythromycin, Clavulanic acid, Ciprofloxacin, Clofazimine, Fluconazole, Linezolid, and Moxifloxacin were meticulously considered for development and validation in the chicken tissues. These antimicrobials were chosen based on their everyday use in poultry farming and their potential impact on human health. We used Ultra-Performance Liquid Chromatography with triple quad Mass Spectrometry and employed multiple reaction monitoring to detect the analytes of interest. All the compounds were well separated using Atlantis T3, 4.6x50mm, 3 μm. The linear range was set between 25 to 1000 nggm. The method was validated following linearity, extraction recovery, matrix effect impact, limit of detection, sensitivity, autosampler and benchtop stability, ensuring the results reliability and our methods robustness.

 A novel and significant method was developed and validated with a sensitive, rapid, and simultaneous analytical method to determine antimicrobials in chicken tissues such as the kidney and liver. The process involved a unique approach to precipitation extraction. This method has not been widely used in this context, followed by the evaporation of the supernatant and reconstitution with the mobile phase. Antimicrobials, including Azithromycin, Clarithromycin, Erythromycin, Clavulanic acid, Ciprofloxacin, Clofazimine, Fluconazole, Linezolid, and Moxifloxacin were meticulously considered for development and validation in the chicken tissues. These antimicrobials were chosen based on their everyday use in poultry farming and their potential impact on human health. We used Ultra-Performance Liquid Chromatography with triple quad Mass Spectrometry and employed multiple reaction monitoring to detect the analytes of interest. All the compounds were well separated using Atlantis T3, 4.6x50mm, 3 μm. The linear range was set between 25 to 1000 nggm. The method was validated following linearity, extraction recovery, matrix effect impact, limit of detection, sensitivity, autosampler and benchtop stability, ensuring the results reliability and our methods robustness.

 A novel and significant method was developed and validated with a sensitive, rapid, and simultaneous analytical method to determine antimicrobials in chicken tissues such as the kidney and liver. The process involved a unique approach to precipitation extraction. This method has not been widely used in this context, followed by the evaporation of the supernatant and reconstitution with the mobile phase. Antimicrobials, including Azithromycin, Clarithromycin, Erythromycin, Clavulanic acid, Ciprofloxacin, Clofazimine, Fluconazole, Linezolid, and Moxifloxacin were meticulously considered for development and validation in the chicken tissues. These antimicrobials were chosen based on their everyday use in poultry farming and their potential impact on human health. We used Ultra-Performance Liquid Chromatography with triple quad Mass Spectrometry and employed multiple reaction monitoring to detect the analytes of interest. All the compounds were well separated using Atlantis T3, 4.6x50mm, 3 μm. The linear range was set between 25 to 1000 nggm. The method was validated following linearity, extraction recovery, matrix effect impact, limit of detection, sensitivity, autosampler and benchtop stability, ensuring the results reliability and our methods robustness.

 A novel and significant method was developed and validated with a sensitive, rapid, and simultaneous analytical method to determine antimicrobials in chicken tissues such as the kidney and liver. The process involved a unique approach to precipitation extraction. This method has not been widely used in this context, followed by the evaporation of the supernatant and reconstitution with the mobile phase. Antimicrobials, including Azithromycin, Clarithromycin, Erythromycin, Clavulanic acid, Ciprofloxacin, Clofazimine, Fluconazole, Linezolid, and Moxifloxacin were meticulously considered for development and validation in the chicken tissues. These antimicrobials were chosen based on their everyday use in poultry farming and their potential impact on human health. We used Ultra-Performance Liquid Chromatography with triple quad Mass Spectrometry and employed multiple reaction monitoring to detect the analytes of interest. All the compounds were well separated using Atlantis T3, 4.6x50mm, 3 μm. The linear range was set between 25 to 1000 nggm. The method was validated following linearity, extraction recovery, matrix effect impact, limit of detection, sensitivity, autosampler and benchtop stability, ensuring the results reliability and our methods robustness.

Makale detayları

Simultaneous Quantification of Multi-Class Antimicrobials in Chicken Kidney and Liver by New Validated UPLC-MS/MS Method

Yazarlar:

Sathish Kumar Konidala | Shridhar Narayanan

Yayıncı:

Hacettepe University

Ana Başlıklar:

Pharmaceutical Analytical Chemistry

Dergi:

Hacettepe University Journal of the Faculty of Pharmacy Vol.45 Issue 1, pp.30-42

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001341854

pISSN:

2458-8806

Antimicrobials

Antimicrobial resistance

chicken tissues

method development

validation

Sayı

Makale detayları

Simultaneous Quantification of Multi-Class Antimicrobials in Chicken Kidney and Liver by New Validated UPLC-MS/MS Method

Yazarlar: Sathish Kumar Konidala | Shridhar Narayanan

Yayıncı: Hacettepe University

Ana Başlıklar: Pharmaceutical Analytical Chemistry

Dergi: Hacettepe University Journal of the Faculty of Pharmacy Vol.45 Issue 1, pp.30-42

Belge Tipi: Makale

Yayın Tarihi: 1/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001341854

pISSN: 2458-8806

Antimicrobials

Antimicrobial resistance

chicken tissues

method development

validation

Sayı

Yazarlar:

Sathish Kumar Konidala | Shridhar Narayanan

Yayıncı:

Hacettepe University

Ana Başlıklar:

Pharmaceutical Analytical Chemistry

Dergi:

Hacettepe University Journal of the Faculty of Pharmacy Vol.45 Issue 1, pp.30-42

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001341854

pISSN:

2458-8806