Identification and cloning of highly epitopic regions of Clostridium novyi alpha toxin

NAJME GORD NOSHAHRI; MOHSEN FATHI NAJAFI; ALI MAKHDOUMI KAKHKI; BEHJAT MAJIDI; MOHSEN MEHRVARZ

Makale detayları

Identification and cloning of highly epitopic regions of Clostridium novyi alpha toxin

Yazarlar:

NAJME GORD NOSHAHRI | MOHSEN FATHI NAJAFI | ALI MAKHDOUMI KAKHKI | BEHJAT MAJIDI | MOHSEN MEHRVARZ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2016 Vol.40 Issue 6, pp.1219-1226

Belge Tipi:

Makale

Yayın Tarihi:

11/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000811525

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Özet:

 

The aim of the present study is to provide a strategy for predicting the production of antigenic regions of Clostridium novyi alpha toxin. The selection is based on B-cell epitopes and MHCII binding protein by immunoinformatics tools. The study resulted in identifying antigenic regions in the beginning and middle (3-17 and 965-997 amino acid residues) as suitable binders to MHCII and the carboxyl terminal of the protein (1800-1958 amino acid residues) in B-cell epitopes. The appropriate region in B-cell epitopes was chosen for cloning. The presence of recombinant protein was detected with immunological methods. Subsequently, BALB/C mice were immunized with the recombinant protein and alpha toxin, and the antibodies produced were evaluated by dot-blot and ELISA tests. After cloning the highly antigenic region (1799-1966 amino acid residues), the results of immunological tests showed that the recombinant protein reacts with antitoxin antibodies. Antirecombinant protein has a higher affinity to the alpha toxin than antialpha toxin. Thus, the carboxyl terminal of the protein (1799-1966 amino acid residues) in B-cell epitopes could be a proper candidate for a peptide vaccine against alpha toxin.

Makale detayları

Identification and cloning of highly epitopic regions of Clostridium novyi alpha toxin

Yazarlar: NAJME GORD NOSHAHRI | MOHSEN FATHI NAJAFI | ALI MAKHDOUMI KAKHKI | BEHJAT MAJIDI | MOHSEN MEHRVARZ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2016 Vol.40 Issue 6, pp.1219-1226

Belge Tipi: Makale

Yayın Tarihi: 11/2016

Belge Dili: Türkçe

Document ID (Org No.): 2000811525

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

NAJME GORD NOSHAHRI | MOHSEN FATHI NAJAFI | ALI MAKHDOUMI KAKHKI | BEHJAT MAJIDI | MOHSEN MEHRVARZ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2016 Vol.40 Issue 6, pp.1219-1226

Belge Tipi:

Makale

Yayın Tarihi:

11/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000811525

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152