Effects of inactivated Streptococcus iniae, Edwardsiella tarda, and Poly I:C on mRNA Expression Levels of CXCL-10 and CXCL-9 Genes in Japanese Flounder

Ergi Bahrioğlu

Makale detayları

Effects of inactivated Streptococcus iniae, Edwardsiella tarda, and Poly I:C on mRNA Expression Levels of CXCL-10 and CXCL-9 Genes in Japanese Flounder

Yazarlar:

Ergi Bahrioğlu

Yayıncı:

Isparta University of Applied Sciences

Ana Başlıklar:

Yapısal Biyoloji

Dergi:

Acta Aquatica Turcica Vol.20 Issue 2, pp.128-139

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001317751

pISSN:

2651-5474

Anahtar kelimeler:

Özet:

 The present study accomplished the successful cloning and sequencing of the JfCXCL9_L and JfCXCL10_L genes found in the spleen cDNA of the Japanese flounder Paralichthys olivaceus. The tissue distribution of these two genes was determined before any stimuli administration at the zero-hour mark. The qRT-PCR analysis demonstrated that the expression of JfCXCL10_L closely mirrored that of IL1-β, displaying an upregulation following the application of Poly I:C Viral mimic and formalin-killed Edwardsiella tarda Gram-Negative mimic, while showing a downregulation after the application of formalin-killed Streptococcus iniae Gram-Positive mimic treatment. These findings strongly suggest a role for JfCXCL10_L in the immune response to viral and gram-negative bacterial stimuli. Regarding JfCXCL9_L, mRNA levels were found to be significantly downregulated after FKCET and FKCSI treatments, though to varying extents. Interestingly, at specific time points, JfCXCL9_L levels were even lower compared to Poly I:C treatment. These intriguing findings shed valuable light on the roles of both JfCXCL9_L and JfCXCL10_L in potential functions of immune response mechanisms of the Japanese flounder.

 The present study accomplished the successful cloning and sequencing of the JfCXCL9_L and JfCXCL10_L genes found in the spleen cDNA of the Japanese flounder Paralichthys olivaceus. The tissue distribution of these two genes was determined before any stimuli administration at the zero-hour mark. The qRT-PCR analysis demonstrated that the expression of JfCXCL10_L closely mirrored that of IL1-β, displaying an upregulation following the application of Poly I:C Viral mimic and formalin-killed Edwardsiella tarda Gram-Negative mimic, while showing a downregulation after the application of formalin-killed Streptococcus iniae Gram-Positive mimic treatment. These findings strongly suggest a role for JfCXCL10_L in the immune response to viral and gram-negative bacterial stimuli. Regarding JfCXCL9_L, mRNA levels were found to be significantly downregulated after FKCET and FKCSI treatments, though to varying extents. Interestingly, at specific time points, JfCXCL9_L levels were even lower compared to Poly I:C treatment. These intriguing findings shed valuable light on the roles of both JfCXCL9_L and JfCXCL10_L in potential functions of immune response mechanisms of the Japanese flounder.

 The present study accomplished the successful cloning and sequencing of the JfCXCL9_L and JfCXCL10_L genes found in the spleen cDNA of the Japanese flounder Paralichthys olivaceus. The tissue distribution of these two genes was determined before any stimuli administration at the zero-hour mark. The qRT-PCR analysis demonstrated that the expression of JfCXCL10_L closely mirrored that of IL1-β, displaying an upregulation following the application of Poly I:C Viral mimic and formalin-killed Edwardsiella tarda Gram-Negative mimic, while showing a downregulation after the application of formalin-killed Streptococcus iniae Gram-Positive mimic treatment. These findings strongly suggest a role for JfCXCL10_L in the immune response to viral and gram-negative bacterial stimuli. Regarding JfCXCL9_L, mRNA levels were found to be significantly downregulated after FKCET and FKCSI treatments, though to varying extents. Interestingly, at specific time points, JfCXCL9_L levels were even lower compared to Poly I:C treatment. These intriguing findings shed valuable light on the roles of both JfCXCL9_L and JfCXCL10_L in potential functions of immune response mechanisms of the Japanese flounder.

 Bu çalışma, Japon pisi balığının Paralichthys olivaceus dalak cDNAsında JfCXCL9_L ve JfCXCL10_L genlerinin başarılı bir şekilde klonlandığını ve dizilendiğini bildirmektedir. Bu iki genin dokulardaki dağılımları da herhangi bir stimulant uygulanmadan önce 0h belirlenmiştir. Ayrıca, qRT-PCR analizi, JfCXCL10_L ekspresyonlarının IL1-β ile benzerlik gösterdiğini ortaya koymuştur. Poly I:C ve formalinle öldürülmüş Edwardsiella tarda FKCET uygulamasından sonra artan ekspresyon seviyesi gösterirken, formalinle öldürülmüş Streptococcus iniae FKCSI uygulamasından sonra azalan ekpresyon seviyesi tespit edilmiştir. Bu durum, JfCXCL10_Lnin viral ve gram-negatif bakteriyel uyarılara karşı bağışıklık tepkisinde rolü olduğunu düşündürmektedir. JfCXCL9_L mRNA düzeyleri, FKCET ve FKCSI uygulamalarından sonra anlamlı derecede düşmüştür. Özellikle belirli zaman noktalarında, JfCXCL9_L düzeyleri Poly I:C uygulamasına kıyasla daha düşük bulunmuştur. Bu bulgular, JfCXCL9_L ve JfCXCL10_Lnin Japon pisi balığında bağışıklık tepkisi mekanizmaları ve potansiyel işlevleri konusunda bilgi sunmaktadır.

Makale detayları

Effects of inactivated Streptococcus iniae, Edwardsiella tarda, and Poly I:C on mRNA Expression Levels of CXCL-10 and CXCL-9 Genes in Japanese Flounder

Yazarlar:

Ergi Bahrioğlu

Yayıncı:

Isparta University of Applied Sciences

Ana Başlıklar:

Yapısal Biyoloji

Dergi:

Acta Aquatica Turcica Vol.20 Issue 2, pp.128-139

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001317751

pISSN:

2651-5474

CXC Motif

Chemokine

Poly I:C

Paralichthys olivaceus

Gene ExpressionCXC Motifi

Kemokin

Poly I:C

Paralichthys olivaceus

Gen Ekspresyonu

Sayı

Makale detayları

Yazarlar: Ergi Bahrioğlu

Yayıncı: Isparta University of Applied Sciences

Ana Başlıklar: Yapısal Biyoloji

Dergi: Acta Aquatica Turcica Vol.20 Issue 2, pp.128-139

Belge Tipi: Makale

Yayın Tarihi: 1/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001317751

pISSN: 2651-5474

Sayı

Yazarlar:

Ergi Bahrioğlu

Yayıncı:

Isparta University of Applied Sciences

Ana Başlıklar:

Yapısal Biyoloji

Dergi:

Acta Aquatica Turcica Vol.20 Issue 2, pp.128-139

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001317751

pISSN:

2651-5474