Probing the architecture of testis and morphology of male germinal cells in the mud crab with the atomic force microscopy

Mahalingam ANBARASU; Ramangalingam KIRUBAGARAN; Gopal DHARANI; Thanumalaya SUBRAMONIAM

Makale detayları

Probing the architecture of testis and morphology of male germinal cells in the mud crab with the atomic force microscopy

Yazarlar:

Mahalingam ANBARASU | Ramangalingam KIRUBAGARAN | Gopal DHARANI | Thanumalaya SUBRAMONIAM

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 5, pp.507-513

Belge Tipi:

Makale

Yayın Tarihi:

4/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000812346

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

The architecture of testicular lobules, morphology of spermatozoa, and spermatophore in the mud crab, Scylla serrata, are described for the first time employing atomic force microscopy (AFM). A fast and simple squash preparation protocol was followed using crustacean physiological saline without distorting the natural structure. Observations provided new insight into the arrangement of lobules and morphology of testicular assembly. It consists of a close network of lobules of different sizes in a radial manner on radially branching basal tubules. Budding of new lobules is another distinguishable feature noted in the network of testicular organization. A thin membranous envelope of the lobule with undulating processes offered indirect evidence on the possible storage of sperm cells within the lumen of the lobule. Spermatozoa of S. serrata were of different shapes and sizes, and, depending on the maturational status, they displayed changes in the acrosome vesicle with a significant amount of cytoplasm. Vertical constrictions found in the testicular lobules suggest propulsive extrusion of spermatophoric masses into the central lumen. These observations necessitate further study using AFM and involving molecular probes to elucidate the polymorphic nature of male germinal cells, mechanisms of spermatophore formation, and the phylogenetic relationship among crustaceans in general.

 

The architecture of testicular lobules, morphology of spermatozoa, and spermatophore in the mud crab, Scylla serrata, are described for the first time employing atomic force microscopy (AFM). A fast and simple squash preparation protocol was followed using crustacean physiological saline without distorting the natural structure. Observations provided new insight into the arrangement of lobules and morphology of testicular assembly. It consists of a close network of lobules of different sizes in a radial manner on radially branching basal tubules. Budding of new lobules is another distinguishable feature noted in the network of testicular organization. A thin membranous envelope of the lobule with undulating processes offered indirect evidence on the possible storage of sperm cells within the lumen of the lobule. Spermatozoa of S. serrata were of different shapes and sizes, and, depending on the maturational status, they displayed changes in the acrosome vesicle with a significant amount of cytoplasm. Vertical constrictions found in the testicular lobules suggest propulsive extrusion of spermatophoric masses into the central lumen. These observations necessitate further study using AFM and involving molecular probes to elucidate the polymorphic nature of male germinal cells, mechanisms of spermatophore formation, and the phylogenetic relationship among crustaceans in general.

Makale detayları

Probing the architecture of testis and morphology of male germinal cells in the mud crab with the atomic force microscopy

Yazarlar:

Mahalingam ANBARASU | Ramangalingam KIRUBAGARAN | Gopal DHARANI | Thanumalaya SUBRAMONIAM

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 5, pp.507-513

Belge Tipi:

Makale

Yayın Tarihi:

4/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000812346

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Key words: Testis

spermatozoa

spermatophore

Scylla serrata

AFM

Sayı

Makale detayları

Probing the architecture of testis and morphology of male germinal cells in the mud crab with the atomic force microscopy

Yazarlar: Mahalingam ANBARASU | Ramangalingam KIRUBAGARAN | Gopal DHARANI | Thanumalaya SUBRAMONIAM

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2013 Vol.37 Issue 5, pp.507-513

Belge Tipi: Makale

Yayın Tarihi: 4/2013

Belge Dili: Türkçe

Document ID (Org No.): 2000812346

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Key words: Testis

spermatozoa

spermatophore

Scylla serrata

AFM

Sayı

Yazarlar:

Mahalingam ANBARASU | Ramangalingam KIRUBAGARAN | Gopal DHARANI | Thanumalaya SUBRAMONIAM

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 5, pp.507-513

Belge Tipi:

Makale

Yayın Tarihi:

4/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000812346

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152