Isolation and biotyping of Brucella melitensis from aborted sheep and goat fetuses

Esra BÜYÜKCANGAZ; Ayşin ŞEN; Serpil KAHYA

Makale detayları

Isolation and biotyping of Brucella melitensis from aborted sheep and goat fetuses

Yazarlar:

Esra BÜYÜKCANGAZ | Ayşin ŞEN | Serpil KAHYA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2009 Vol.33 Issue 4, pp.311-316

Belge Tipi:

Makale

Yayın Tarihi:

3/2009

Belge Dili:

Türkçe

Document ID (Org No.):

2000767706

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

The possible role of Brucella spp. in 65 abortion cases, 55 from sheep and 10 from goats, occurring in the birth seasons of 2004 and 2005 in northwestern Turkey was investigated. Colony morphology, agglutination by acriflavin, H2S production, CO2 requirement, dye sensitivity in thionin, basic fuchsin, growth characteristics in streptomycin, lysis with Tbilisi phage, and agglutination with monospecific A- and M antisera were examined for identification and biotyping. The isolates from 21 of 55 sheep and 1 of 10 goat abortion cases were identified as Brucella melitensis. Biotyping revealed that 14, 6, and 1 of 22 Brucella spp. were B. melitensis biotype 3, B. melitensis biotype 1, and B. melitensis biotype 2, respectively. One strain isolated from a sheep abortion case was H2S- producing and was identified as atypical B. melitensis.

 

The possible role of Brucella spp. in 65 abortion cases, 55 from sheep and 10 from goats, occurring in the birth seasons of 2004 and 2005 in northwestern Turkey was investigated. Colony morphology, agglutination by acriflavin, H2S production, CO2 requirement, dye sensitivity in thionin, basic fuchsin, growth characteristics in streptomycin, lysis with Tbilisi phage, and agglutination with monospecific A- and M antisera were examined for identification and biotyping. The isolates from 21 of 55 sheep and 1 of 10 goat abortion cases were identified as Brucella melitensis. Biotyping revealed that 14, 6, and 1 of 22 Brucella spp. were B. melitensis biotype 3, B. melitensis biotype 1, and B. melitensis biotype 2, respectively. One strain isolated from a sheep abortion case was H2S- producing and was identified as atypical B. melitensis.

Makale detayları

Isolation and biotyping of Brucella melitensis from aborted sheep and goat fetuses

Yazarlar:

Esra BÜYÜKCANGAZ | Ayşin ŞEN | Serpil KAHYA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2009 Vol.33 Issue 4, pp.311-316

Belge Tipi:

Makale

Yayın Tarihi:

3/2009

Belge Dili:

Türkçe

Document ID (Org No.):

2000767706

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Key words: Brucella melitensis

sheep

goat

abortus

biotyping

Sayı

Makale detayları

Isolation and biotyping of Brucella melitensis from aborted sheep and goat fetuses

Yazarlar: Esra BÜYÜKCANGAZ | Ayşin ŞEN | Serpil KAHYA

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2009 Vol.33 Issue 4, pp.311-316

Belge Tipi: Makale

Yayın Tarihi: 3/2009

Belge Dili: Türkçe

Document ID (Org No.): 2000767706

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Key words: Brucella melitensis

sheep

goat

abortus

biotyping

Sayı

Yazarlar:

Esra BÜYÜKCANGAZ | Ayşin ŞEN | Serpil KAHYA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2009 Vol.33 Issue 4, pp.311-316

Belge Tipi:

Makale

Yayın Tarihi:

3/2009

Belge Dili:

Türkçe

Document ID (Org No.):

2000767706

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128