Comparison of behavioural and molecular effects of two different schizophrenia models induced by subchronic MK-801 administration in rats

Gökhan Ünal; Feyza Arıcıoğlu

Makale detayları

Comparison of behavioural and molecular effects of two different schizophrenia models induced by subchronic MK-801 administration in rats

Yazarlar:

Gökhan Ünal | Feyza Arıcıoğlu

Yayıncı:

Marmara University

Ana Başlıklar:

Basic Pharmacology

Dergi:

Journal of Research in Pharmacy Vol.22 Issue 2, pp.199-208

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346173

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 Schizophrenia is a severe psychiatric disorder with about 1 prevalance. NMDA receptor antagonists such as Phencyclidine PCP and MK-801 are commonly used for modeling schizophrenia in rodents. In literature, despite of the concensus about subchronic PCP administration commonly 7 days, bi-daily administration followed by a 1 week washout period, there are different subchronic administration regimens for MK-801 beside 7 days, bidaily MK-801-7, such as 14 days MK-801-14 daily or 28 days daily injections. In this study, we aimed to compare two prevalant MK-801 models MK-801-7 and MK-801-14, 0.2 mgkg in both behavioural and molecular changes. Wistar Hannover rats grouped as control n10, MK-801-14 n8 and MK-801-7 n8. Prepulse inhibition of acustic startle response PPI, novel object recognition test NORT, social interaction SI and Morriss water maze MWM tests were used for behavioural analyzes while real time polimerase chain reaction Rt-PCR was conducted for molecular analyzes of glutamic acid decarboxilase 67 GAD67 and parvalbumin. Our results showed decreased PPI in MK-801-14 and MK801-7 groups. Moreover, in both models platform finding latencies were increased and swimming time in platform area was decreased in MWM. MK-801-14 and MK-801-7 reduced following and raised avoiding behaviours in SI. In Rt-PCR, GAD67 mRNA levels were decreased by MK-801-14 and MK-801-7 administrations. However, only MK-801-7 decreased discrimination index in NORT and parvalbumin mRNA levels. In this study, it has been showed that although MK-801-14 and MK-801-7 administrations revealed smiliar schizophrenia like symptoms in rats, MK-801-7 has partial superiories in certain aspects.

 Schizophrenia is a severe psychiatric disorder with about 1 prevalance. NMDA receptor antagonists such as Phencyclidine PCP and MK-801 are commonly used for modeling schizophrenia in rodents. In literature, despite of the concensus about subchronic PCP administration commonly 7 days, bi-daily administration followed by a 1 week washout period, there are different subchronic administration regimens for MK-801 beside 7 days, bidaily MK-801-7, such as 14 days MK-801-14 daily or 28 days daily injections. In this study, we aimed to compare two prevalant MK-801 models MK-801-7 and MK-801-14, 0.2 mgkg in both behavioural and molecular changes. Wistar Hannover rats grouped as control n10, MK-801-14 n8 and MK-801-7 n8. Prepulse inhibition of acustic startle response PPI, novel object recognition test NORT, social interaction SI and Morriss water maze MWM tests were used for behavioural analyzes while real time polimerase chain reaction Rt-PCR was conducted for molecular analyzes of glutamic acid decarboxilase 67 GAD67 and parvalbumin. Our results showed decreased PPI in MK-801-14 and MK801-7 groups. Moreover, in both models platform finding latencies were increased and swimming time in platform area was decreased in MWM. MK-801-14 and MK-801-7 reduced following and raised avoiding behaviours in SI. In Rt-PCR, GAD67 mRNA levels were decreased by MK-801-14 and MK-801-7 administrations. However, only MK-801-7 decreased discrimination index in NORT and parvalbumin mRNA levels. In this study, it has been showed that although MK-801-14 and MK-801-7 administrations revealed smiliar schizophrenia like symptoms in rats, MK-801-7 has partial superiories in certain aspects.

 Schizophrenia is a severe psychiatric disorder with about 1 prevalance. NMDA receptor antagonists such as Phencyclidine PCP and MK-801 are commonly used for modeling schizophrenia in rodents. In literature, despite of the concensus about subchronic PCP administration commonly 7 days, bi-daily administration followed by a 1 week washout period, there are different subchronic administration regimens for MK-801 beside 7 days, bidaily MK-801-7, such as 14 days MK-801-14 daily or 28 days daily injections. In this study, we aimed to compare two prevalant MK-801 models MK-801-7 and MK-801-14, 0.2 mgkg in both behavioural and molecular changes. Wistar Hannover rats grouped as control n10, MK-801-14 n8 and MK-801-7 n8. Prepulse inhibition of acustic startle response PPI, novel object recognition test NORT, social interaction SI and Morriss water maze MWM tests were used for behavioural analyzes while real time polimerase chain reaction Rt-PCR was conducted for molecular analyzes of glutamic acid decarboxilase 67 GAD67 and parvalbumin. Our results showed decreased PPI in MK-801-14 and MK801-7 groups. Moreover, in both models platform finding latencies were increased and swimming time in platform area was decreased in MWM. MK-801-14 and MK-801-7 reduced following and raised avoiding behaviours in SI. In Rt-PCR, GAD67 mRNA levels were decreased by MK-801-14 and MK-801-7 administrations. However, only MK-801-7 decreased discrimination index in NORT and parvalbumin mRNA levels. In this study, it has been showed that although MK-801-14 and MK-801-7 administrations revealed smiliar schizophrenia like symptoms in rats, MK-801-7 has partial superiories in certain aspects.

 Schizophrenia is a severe psychiatric disorder with about 1 prevalance. NMDA receptor antagonists such as Phencyclidine PCP and MK-801 are commonly used for modeling schizophrenia in rodents. In literature, despite of the concensus about subchronic PCP administration commonly 7 days, bi-daily administration followed by a 1 week washout period, there are different subchronic administration regimens for MK-801 beside 7 days, bidaily MK-801-7, such as 14 days MK-801-14 daily or 28 days daily injections. In this study, we aimed to compare two prevalant MK-801 models MK-801-7 and MK-801-14, 0.2 mgkg in both behavioural and molecular changes. Wistar Hannover rats grouped as control n10, MK-801-14 n8 and MK-801-7 n8. Prepulse inhibition of acustic startle response PPI, novel object recognition test NORT, social interaction SI and Morriss water maze MWM tests were used for behavioural analyzes while real time polimerase chain reaction Rt-PCR was conducted for molecular analyzes of glutamic acid decarboxilase 67 GAD67 and parvalbumin. Our results showed decreased PPI in MK-801-14 and MK801-7 groups. Moreover, in both models platform finding latencies were increased and swimming time in platform area was decreased in MWM. MK-801-14 and MK-801-7 reduced following and raised avoiding behaviours in SI. In Rt-PCR, GAD67 mRNA levels were decreased by MK-801-14 and MK-801-7 administrations. However, only MK-801-7 decreased discrimination index in NORT and parvalbumin mRNA levels. In this study, it has been showed that although MK-801-14 and MK-801-7 administrations revealed smiliar schizophrenia like symptoms in rats, MK-801-7 has partial superiories in certain aspects.

Makale detayları

Comparison of behavioural and molecular effects of two different schizophrenia models induced by subchronic MK-801 administration in rats

Yazarlar:

Gökhan Ünal | Feyza Arıcıoğlu

Yayıncı:

Marmara University

Ana Başlıklar:

Basic Pharmacology

Dergi:

Journal of Research in Pharmacy Vol.22 Issue 2, pp.199-208

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346173

pISSN:

2630-6344

MK-801

GAD67

Parvalbumin

Schizophrenia

Sayı

Makale detayları

Comparison of behavioural and molecular effects of two different schizophrenia models induced by subchronic MK-801 administration in rats

Yazarlar: Gökhan Ünal | Feyza Arıcıoğlu

Yayıncı: Marmara University

Ana Başlıklar: Basic Pharmacology

Dergi: Journal of Research in Pharmacy Vol.22 Issue 2, pp.199-208

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001346173

pISSN: 2630-6344

MK-801

GAD67

Parvalbumin

Schizophrenia

Sayı

Yazarlar:

Gökhan Ünal | Feyza Arıcıoğlu

Yayıncı:

Marmara University

Ana Başlıklar:

Basic Pharmacology

Dergi:

Journal of Research in Pharmacy Vol.22 Issue 2, pp.199-208

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346173

pISSN:

2630-6344