Molecular characterization and comparative genomic analysis of two triamitovirus isolates hosted by the hypogean fungus Tuber excavatum Vittad.

Elifnaz Bora; Ilgaz Akata; Emre Keskin; Ergin Sahin

Makale detayları

Molecular characterization and comparative genomic analysis of two triamitovirus isolates hosted by the hypogean fungus Tuber excavatum Vittad.

Yazarlar:

Elifnaz Bora | Ilgaz Akata | Emre Keskin | Ergin Sahin

Yayıncı:

Trakya University

Ana Başlıklar:

Genomics and Transcriptomics | Phylogeny and Comparative Analysis | Molecular Evolution | Virology

Dergi:

Trakya University Journal of Natural Sciences Vol.25 Issue 2, pp.169-176

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001324503

pISSN:

2528-9691

Anahtar kelimeler:

Özet:

 The connections between viruses and their hosts are complex and can arise from any combination of different evolutionary events including codivergence, switching, and duplication of the pathogen. Mycoviruses, a diverse virus group whose members specifically infect fungal hosts, are subject to similar evolutionary processes. In this study, we present the identification and complete genome characterization of the second isolate of a mitovirus, commonly known as Tuber excavatum mitovirus, officially named Triamitovirus tuex1. This mycovirus infects the hypogean, ectomyrrhizal fungus Tuber excavatum Vittad.. Both Triamitovirus tuex1 isolates, Tekirdağ identified by us and Lammspringe, were found in the fruiting bodies of T. excavatum isolates collected from Türkiye and Germany, respectively. Comparative genomic analyses revealed that the two virus isolates share 85.33 sequence similarity in their whole genomes, with their protein encompassing RNA-dependent RNA polymerase RdRp domain showing an identity rate of 94.60. The most diverse part of the viral genomes was found to be the 5 untranslated regions UTRs, with a sequence similarity of 78.53, while the 3 UTRs were the most conserved, with 91.53 sequence similarity. Considering the shared host species, the emergence of these Triamitovirus tuex1 isolates appears to reflect a duplication pattern intra-host divergence resulting from adaptive radiation.

 The connections between viruses and their hosts are complex and can arise from any combination of different evolutionary events including codivergence, switching, and duplication of the pathogen. Mycoviruses, a diverse virus group whose members specifically infect fungal hosts, are subject to similar evolutionary processes. In this study, we present the identification and complete genome characterization of the second isolate of a mitovirus, commonly known as Tuber excavatum mitovirus, officially named Triamitovirus tuex1. This mycovirus infects the hypogean, ectomyrrhizal fungus Tuber excavatum Vittad.. Both Triamitovirus tuex1 isolates, Tekirdağ identified by us and Lammspringe, were found in the fruiting bodies of T. excavatum isolates collected from Türkiye and Germany, respectively. Comparative genomic analyses revealed that the two virus isolates share 85.33 sequence similarity in their whole genomes, with their protein encompassing RNA-dependent RNA polymerase RdRp domain showing an identity rate of 94.60. The most diverse part of the viral genomes was found to be the 5 untranslated regions UTRs, with a sequence similarity of 78.53, while the 3 UTRs were the most conserved, with 91.53 sequence similarity. Considering the shared host species, the emergence of these Triamitovirus tuex1 isolates appears to reflect a duplication pattern intra-host divergence resulting from adaptive radiation.

 The connections between viruses and their hosts are complex and can arise from any combination of different evolutionary events including codivergence, switching, and duplication of the pathogen. Mycoviruses, a diverse virus group whose members specifically infect fungal hosts, are subject to similar evolutionary processes. In this study, we present the identification and complete genome characterization of the second isolate of a mitovirus, commonly known as Tuber excavatum mitovirus, officially named Triamitovirus tuex1. This mycovirus infects the hypogean, ectomyrrhizal fungus Tuber excavatum Vittad.. Both Triamitovirus tuex1 isolates, Tekirdağ identified by us and Lammspringe, were found in the fruiting bodies of T. excavatum isolates collected from Türkiye and Germany, respectively. Comparative genomic analyses revealed that the two virus isolates share 85.33 sequence similarity in their whole genomes, with their protein encompassing RNA-dependent RNA polymerase RdRp domain showing an identity rate of 94.60. The most diverse part of the viral genomes was found to be the 5 untranslated regions UTRs, with a sequence similarity of 78.53, while the 3 UTRs were the most conserved, with 91.53 sequence similarity. Considering the shared host species, the emergence of these Triamitovirus tuex1 isolates appears to reflect a duplication pattern intra-host divergence resulting from adaptive radiation.

 Virüsler ile konakları arasındaki bağlantılar karmaşıktır ve kodivergens birlikte ıraksama, değişim ve patojenin çoğaltılması gibi farklı evrimsel olayların herhangi bir kombinasyonundan kaynaklanabilir. Mikovirüsler, özel olarak mantar konaklarını enfekte eden, çeşitlilik gösteren bir virüs grubudur ve benzer evrimsel süreçlere tabidir. Bu çalışmada, Tuber excavatum mitovirüsü olarak bilinen ve resmi olarak Triamitovirus tuex1 olarak adlandırılan bir mitovirüsün ikinci izolatının tanımlanması ve tüm genom nitelemesi sunulmaktadır. Bu mikovirüs, hipogean, ektomikorizal mantar Tuber excavatum Vittad.ı enfekte eder. Sırasıyla Türkiye ve Almanyadan toplanan Tekirdağ bizim tarafımızdan tanımlanan ve Lammspringe Triamitovirus tuex1 izolatlarının her ikisi de, T. excavatum meyvelerinde tanımlandı. Karşılaştırmalı genom analizleri, her iki virüs izolatının da tüm genomlarında 85,33lük bir dizi benzerliği paylaştığını ve RNA bağımlı RNA polimeraz RdRp alanını domain içeren proteinlerinin 94,60lık bir benzerlik oranına sahip olduğunu ortaya koymuştur. Viral genomların en çok farklılık gösteren kısmının 78,53lük bir dizi benzerliği gösteren 5 translasyonu yapılmayan bölgeler UTRler olduğu, 3 UTRlerin ise 91,53lük bir dizi benzerliği ile en çok korunmuş kısımlar olduğu bulunmuştur. Konak türlerin ortak olması göz önünde bulundurulduğunda, bu Triamitovirus tuex1 izolatlarının ortaya çıkışları, adaptif radyasyondan kaynaklanan bir çoğaltma modelini konak-içi çeşitlenmesi yansıtıyor gibi görünmektedir.

Makale detayları

Molecular characterization and comparative genomic analysis of two triamitovirus isolates hosted by the hypogean fungus Tuber excavatum Vittad.

Yazarlar:

Elifnaz Bora | Ilgaz Akata | Emre Keskin | Ergin Sahin

Yayıncı:

Trakya University

Ana Başlıklar:

Genomics and Transcriptomics | Phylogeny and Comparative Analysis | Molecular Evolution | Virology

Dergi:

Trakya University Journal of Natural Sciences Vol.25 Issue 2, pp.169-176

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001324503

pISSN:

2528-9691

Mycovirus

Mitovirus

Virus evolution

Truffle

Tuber excavatum

Sayı

Makale detayları

Molecular characterization and comparative genomic analysis of two triamitovirus isolates hosted by the hypogean fungus Tuber excavatum Vittad.

Yazarlar: Elifnaz Bora | Ilgaz Akata | Emre Keskin | Ergin Sahin

Yayıncı: Trakya University

Ana Başlıklar: Genomics and Transcriptomics | Phylogeny and Comparative Analysis | Molecular Evolution | Virology

Dergi: Trakya University Journal of Natural Sciences Vol.25 Issue 2, pp.169-176

Belge Tipi: Makale

Yayın Tarihi: 10/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001324503

pISSN: 2528-9691

Mycovirus

Mitovirus

Virus evolution

Truffle

Tuber excavatum

Sayı

Yazarlar:

Elifnaz Bora | Ilgaz Akata | Emre Keskin | Ergin Sahin

Yayıncı:

Trakya University

Ana Başlıklar:

Genomics and Transcriptomics | Phylogeny and Comparative Analysis | Molecular Evolution | Virology

Dergi:

Trakya University Journal of Natural Sciences Vol.25 Issue 2, pp.169-176

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001324503

pISSN:

2528-9691