Purification and Metal Inhibition of Glutathione Reductase Enzyme from Gill Tissue of Scorpionfish

Kübra Işık; Ercan Soydan

Makale detayları

Purification and Metal Inhibition of Glutathione Reductase Enzyme from Gill Tissue of Scorpionfish

Yazarlar:

Kübra Işık | Ercan Soydan

Yayıncı:

Ondokuz Mayıs University

Dergi:

Anadolu Tarım Bilimleri Dergisi Vol.38 Issue 1, pp.221-233

Belge Tipi:

Makale

Yayın Tarihi:

2/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001283744

pISSN:

1308-8750

Anahtar kelimeler:

Özet:

 In this study, glutathione reductase enzyme was partially purified from gill tissue of scorpion fish and the effects of heavy metal ions on enzyme activity were determined. The purification process was achieved in three steps as preparation of homogenate, ammonium sulfate precipitation and dialysis. As a result of the study, optimum pH 6.5, optimum substrate concentration 2 mM NADPH and optimum buffer 400 mM KH 2 PO 4 were determined. After partial purification, the inhibition effects of Cd 2 , Ni 2 , Zn 2 as heavy metal ions were investigated. The IC50 values of heavy metals were calculated as 74 µM, 31 µM, and 56 µM, respectively. The most potent inhibitor was determined to be zinc.

 In this study, glutathione reductase enzyme was partially purified from gill tissue of scorpion fish and the effects of heavy metal ions on enzyme activity were determined. The purification process was achieved in three steps as preparation of homogenate, ammonium sulfate precipitation and dialysis. As a result of the study, optimum pH 6.5, optimum substrate concentration 2 mM NADPH and optimum buffer 400 mM KH 2 PO 4 were determined. After partial purification, the inhibition effects of Cd 2 , Ni 2 , Zn 2 as heavy metal ions were investigated. The IC50 values of heavy metals were calculated as 74 µM, 31 µM, and 56 µM, respectively. The most potent inhibitor was determined to be zinc.

 In this study, glutathione reductase enzyme was partially purified from gill tissue of scorpion fish and the effects of heavy metal ions on enzyme activity were determined. The purification process was achieved in three steps as preparation of homogenate, ammonium sulfate precipitation and dialysis. As a result of the study, optimum pH 6.5, optimum substrate concentration 2 mM NADPH and optimum buffer 400 mM KH 2 PO 4 were determined. After partial purification, the inhibition effects of Cd 2 , Ni 2 , Zn 2 as heavy metal ions were investigated. The IC50 values of heavy metals were calculated as 74 µM, 31 µM, and 56 µM, respectively. The most potent inhibitor was determined to be zinc.

 Bu çalışmada iskorpit balığı solungaç dokusundan glutatyon redüktaz enzimi literatürde ilk kez kısmi olarak saflaştırılmış ve ağır metal iyonlarının enzim aktivitesi üzerindeki etkileri belirlenmiştir. Saflaştırma işlemi homojenat hazırlanması, amonyum sülfat çöktürmesi ve diyaliz olarak üç aşamada gerçekleştirilmiştir. Çalışma sonucunda optimum pH 6.5, optimum substrat konsantrasyonu 2 mM NADPH ve optimum tampon 400 mM KH 2 PO 4 olarak bulunmuştur. Kısmi saflaştırma sonrasında ağır metal iyonları olarak Ni 2 , Zn 2 ve Cd 2 nin inhibisyon etkileri araştırılmış ve ağır metallerin IC50 değerleri sırasıyla 31 µM, 56 µM ve 74 µM olarak hesaplanmıştır. En güçlü inhibitörün çinko oldu tespit edilmiştir.

Makale detayları

Purification and Metal Inhibition of Glutathione Reductase Enzyme from Gill Tissue of Scorpionfish

Yazarlar:

Kübra Işık | Ercan Soydan

Yayıncı:

Ondokuz Mayıs University

Dergi:

Anadolu Tarım Bilimleri Dergisi Vol.38 Issue 1, pp.221-233

Belge Tipi:

Makale

Yayın Tarihi:

2/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001283744

pISSN:

1308-8750

Enzyme purification

glutathione reductase

metal ion

scorpion fish

Enzim saflaştırılması

glutatyon redüktaz

iskorpit balığı

metal iyonları

Sayı

Makale detayları

Yazarlar: Kübra Işık | Ercan Soydan

Yayıncı: Ondokuz Mayıs University

Dergi: Anadolu Tarım Bilimleri Dergisi Vol.38 Issue 1, pp.221-233

Belge Tipi: Makale

Yayın Tarihi: 2/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001283744

pISSN: 1308-8750

Sayı

Yazarlar:

Kübra Işık | Ercan Soydan

Yayıncı:

Ondokuz Mayıs University

Dergi:

Anadolu Tarım Bilimleri Dergisi Vol.38 Issue 1, pp.221-233

Belge Tipi:

Makale

Yayın Tarihi:

2/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001283744

pISSN:

1308-8750