Reference Values for Erythrocyte and Liver Antioxidant Systems in Healthy Male Mice (Mus musculus)

Ergül BELGE KURUTAŞ

Makale detayları

Reference Values for Erythrocyte and Liver Antioxidant Systems in Healthy Male Mice (Mus musculus)

Yazarlar:

Ergül BELGE KURUTAŞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2001 Vol.25 Issue 4, pp.505-509

Belge Tipi:

Makale

Yayın Tarihi:

3/2001

Belge Dili:

Türkçe

Document ID (Org No.):

2000771251

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

The aim of the present study was to define the reference values for erythrocyte and liver tissue glucose-6-phosphate dehydrogenase (G6PDH), glutathione peroxidase (GPX), glutathione reductase (GR) and superoxide dismutase (SOD) activities and also reduced glutathione (GSH) levels in order to obtain basal data for subsequent toxicological investigations. In addition, the best storage conditions were determined for liver G6PDH enzyme.  Male, apparently healthy Mus musculus (38) speciemens that had normal livers (histopathological data) were included in the study. G6PDH and GPX activities and GSH levels were measured by the Beutler method, and SOD aktivity was measured by the Fridovich method in the erythrocyte and liver tissues. GR activity in the erythrocyte was measured by the Beutler method, and GR activity in the liver was measured by the Staal method. The mean value (SD) of the levels of the enzymes and reduced glutathione in erythrocyte and liver tissues were as follows: G6PDH: 22.28 (3.53) U/g Hb, 2.38 (1.09)  U/g liver; GPX: 134.88 (16.83)  U/g Hb, 22.34 (4.70) U/g liver; GR:7.23 (1.58) U/g Hb, 2.47 (1.31) U/g liver; SOD: 4062.94 (929.41) U/g Hb, 553.71 (214.25) U/g liver; and GSH: 9.70 (1.64) µmol/g Hb, 0.94 (0.22) µmol/g liver. The best storage conditions for the liver the G6PDH enzyme were seven days at +4 ºC, thirty days at -70 ºC and thirty days at -20 ºC.

 

The aim of the present study was to define the reference values for erythrocyte and liver tissue glucose-6-phosphate dehydrogenase (G6PDH), glutathione peroxidase (GPX), glutathione reductase (GR) and superoxide dismutase (SOD) activities and also reduced glutathione (GSH) levels in order to obtain basal data for subsequent toxicological investigations. In addition, the best storage conditions were determined for liver G6PDH enzyme.  Male, apparently healthy Mus musculus (38) speciemens that had normal livers (histopathological data) were included in the study. G6PDH and GPX activities and GSH levels were measured by the Beutler method, and SOD aktivity was measured by the Fridovich method in the erythrocyte and liver tissues. GR activity in the erythrocyte was measured by the Beutler method, and GR activity in the liver was measured by the Staal method. The mean value (SD) of the levels of the enzymes and reduced glutathione in erythrocyte and liver tissues were as follows: G6PDH: 22.28 (3.53) U/g Hb, 2.38 (1.09)  U/g liver; GPX: 134.88 (16.83)  U/g Hb, 22.34 (4.70) U/g liver; GR:7.23 (1.58) U/g Hb, 2.47 (1.31) U/g liver; SOD: 4062.94 (929.41) U/g Hb, 553.71 (214.25) U/g liver; and GSH: 9.70 (1.64) µmol/g Hb, 0.94 (0.22) µmol/g liver. The best storage conditions for the liver the G6PDH enzyme were seven days at +4 ºC, thirty days at -70 ºC and thirty days at -20 ºC.

Makale detayları

Reference Values for Erythrocyte and Liver Antioxidant Systems in Healthy Male Mice (Mus musculus)

Yazarlar:

Ergül BELGE KURUTAŞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2001 Vol.25 Issue 4, pp.505-509

Belge Tipi:

Makale

Yayın Tarihi:

3/2001

Belge Dili:

Türkçe

Document ID (Org No.):

2000771251

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Mus musculus

erythrocyte

liver

antioxidant systems

reference values

Sayı

Makale detayları

Reference Values for Erythrocyte and Liver Antioxidant Systems in Healthy Male Mice (Mus musculus)

Yazarlar: Ergül BELGE KURUTAŞ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2001 Vol.25 Issue 4, pp.505-509

Belge Tipi: Makale

Yayın Tarihi: 3/2001

Belge Dili: Türkçe

Document ID (Org No.): 2000771251

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Mus musculus

erythrocyte

liver

antioxidant systems

reference values

Sayı

Yazarlar:

Ergül BELGE KURUTAŞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2001 Vol.25 Issue 4, pp.505-509

Belge Tipi:

Makale

Yayın Tarihi:

3/2001

Belge Dili:

Türkçe

Document ID (Org No.):

2000771251

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128