DETERMINATION OF ANTI-CANCER DRUG PALBOCICLIB FROM HUMAN BIOLOGICAL FLUIDS BY USING DIFFERENTIAL PULSE VOLTAMMETRIC METHOD AT BORON DOPED DIAMOND ELECTRODE

Melike Akan; Çiğdem Kanbeş Dindar; Nazife Aslan; Bengi Uslu

Makale detayları

DETERMINATION OF ANTI-CANCER DRUG PALBOCICLIB FROM HUMAN BIOLOGICAL FLUIDS BY USING DIFFERENTIAL PULSE VOLTAMMETRIC METHOD AT BORON DOPED DIAMOND ELECTRODE

Yazarlar:

Melike Akan | Çiğdem Kanbeş Dindar | Nazife Aslan | Bengi Uslu

Yayıncı:

Ankara University

Ana Başlıklar:

Eczacılıkta Analitik Kimya

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 3, pp.1058-1067

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001319090

pISSN:

1015-3918

Anahtar kelimeler:

Özet:

 Objective: A very efficient and sensitive electrochemical technique utilizing differential pulse voltammetry DPV at a boron-doped diamond electrode BDDE was devised to measure Palbociclib in this study. Material and Method: All experiments employed typical three-electrode cell of 10 ml capacity in conjunction with boron-doped diamond electrode, a platinum wire counter electrode, and an AgAgCl reference electrode. During electrochemical measurements, DPV and cyclic voltammetry CV methods was utilized at BDDE. Result and Discussion: Based on experimental findings from electrochemical characterization investigations, it was determined that oxidation behavior of Palbociclib in BDDE is irreversible and regulated by diffusion. Anodic peak current exhibited a linear relationship within concentration range of 0.011 µM, 0.020.8 µM, and 0.020.8 µM in pH 2.0 phosphate buffer solution PBS for reference substance solution, human serum, and urine samples, respectively. Limits of detection were found as 2.28 nM, 2.93 nM, and 1.31 nM for standard drug solution, human serum and urine samples, respectively. In order to validate the developed method, its repeatability, reproducibility, selectivity, precision and accuracy in all environments were investigated and calculated. This method was successfully applied for the analysis of Palbociclib in human serum and urine samples .

 Objective: A very efficient and sensitive electrochemical technique utilizing differential pulse voltammetry DPV at a boron-doped diamond electrode BDDE was devised to measure Palbociclib in this study. Material and Method: All experiments employed typical three-electrode cell of 10 ml capacity in conjunction with boron-doped diamond electrode, a platinum wire counter electrode, and an AgAgCl reference electrode. During electrochemical measurements, DPV and cyclic voltammetry CV methods was utilized at BDDE. Result and Discussion: Based on experimental findings from electrochemical characterization investigations, it was determined that oxidation behavior of Palbociclib in BDDE is irreversible and regulated by diffusion. Anodic peak current exhibited a linear relationship within concentration range of 0.011 µM, 0.020.8 µM, and 0.020.8 µM in pH 2.0 phosphate buffer solution PBS for reference substance solution, human serum, and urine samples, respectively. Limits of detection were found as 2.28 nM, 2.93 nM, and 1.31 nM for standard drug solution, human serum and urine samples, respectively. In order to validate the developed method, its repeatability, reproducibility, selectivity, precision and accuracy in all environments were investigated and calculated. This method was successfully applied for the analysis of Palbociclib in human serum and urine samples .

 Objective: A very efficient and sensitive electrochemical technique utilizing differential pulse voltammetry DPV at a boron-doped diamond electrode BDDE was devised to measure Palbociclib in this study. Material and Method: All experiments employed typical three-electrode cell of 10 ml capacity in conjunction with boron-doped diamond electrode, a platinum wire counter electrode, and an AgAgCl reference electrode. During electrochemical measurements, DPV and cyclic voltammetry CV methods was utilized at BDDE. Result and Discussion: Based on experimental findings from electrochemical characterization investigations, it was determined that oxidation behavior of Palbociclib in BDDE is irreversible and regulated by diffusion. Anodic peak current exhibited a linear relationship within concentration range of 0.011 µM, 0.020.8 µM, and 0.020.8 µM in pH 2.0 phosphate buffer solution PBS for reference substance solution, human serum, and urine samples, respectively. Limits of detection were found as 2.28 nM, 2.93 nM, and 1.31 nM for standard drug solution, human serum and urine samples, respectively. In order to validate the developed method, its repeatability, reproducibility, selectivity, precision and accuracy in all environments were investigated and calculated. This method was successfully applied for the analysis of Palbociclib in human serum and urine samples .

 Amaç: Bu çalışmada Palbociclibi doğru bir şekilde ölçmek için bor katkılı elmas elektrotta BDDE diferansiyel puls voltametrisi DPV kullanan çok verimli ve hassas bir elektrokimyasal teknik geliştirilmiştir. Gereç ve Yöntem: Tüm deneylerde bor katkılı elmas elektrot, platin tel karşı elektrot ve AgAgCl referans elektrot ile birlikte 10 ml kapasiteli tipik üç elektrotlu hücre kullanılmıştır. Elektrokimyasal ölçümler sırasında BDDEde DPV ve döngüsel voltametri CV yöntemleri kullanılmıştır. Sonuç ve Tartışma: Elektrokimyasal karakterizasyon incelemelerinden elde edilen deneysel bulgulara dayanarak, Palbociclibin BDDEdeki oksidasyon davranışının geri dönüşümsüz olduğu ve difüzyonla düzenlendiği belirlenmiştir. Anodik pik akımı, referans madde çözeltisi, insan serumu ve idrar numuneleri için DPV yöntemiyle pH 2.0 fosfat tampon çözeltisinde PBS sırasıyla 0.01-1 µM, 0.02-0.8µM ve 0.02-0.8 µM konsantrasyon aralığında doğrusal bir ilişki görülmüştür Tespit limitleri standart ilaç çözeltisi, insan serumu ve idrar numuneleri için sırasıyla 2.28 nM, 2.93 nM ve 1.31 nM olarak bulunmuştur. Geliştirilen yöntemin validasyonu için tekrarlanabilirliği, tekrar üretilebilirliği, seçiciliği, kesinliği ve doğruluğu araştırılmış ve hesaplanmıştır. Bu yöntem insan serum ve idrar örneklerinde Palbociclib analizi için başarıyla uygulanmıştır.

Makale detayları

DETERMINATION OF ANTI-CANCER DRUG PALBOCICLIB FROM HUMAN BIOLOGICAL FLUIDS BY USING DIFFERENTIAL PULSE VOLTAMMETRIC METHOD AT BORON DOPED DIAMOND ELECTRODE

Yazarlar:

Melike Akan | Çiğdem Kanbeş Dindar | Nazife Aslan | Bengi Uslu

Yayıncı:

Ankara University

Ana Başlıklar:

Eczacılıkta Analitik Kimya

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 3, pp.1058-1067

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001319090

pISSN:

1015-3918

Boron-doped diamond electrode

differential pulse voltammetry

human serum sample

palbociclib

urine sampleBor katkılı elmas elektrot

diferansiyel puls voltammetri

idrar örneği

insan serum örneği

palbociclib

Sayı

Makale detayları

Yazarlar: Melike Akan | Çiğdem Kanbeş Dindar | Nazife Aslan | Bengi Uslu

Yayıncı: Ankara University

Ana Başlıklar: Eczacılıkta Analitik Kimya

Dergi: Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 3, pp.1058-1067

Belge Tipi: Makale

Yayın Tarihi: 10/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001319090

pISSN: 1015-3918

Sayı

Yazarlar:

Melike Akan | Çiğdem Kanbeş Dindar | Nazife Aslan | Bengi Uslu

Yayıncı:

Ankara University

Ana Başlıklar:

Eczacılıkta Analitik Kimya

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 3, pp.1058-1067

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001319090

pISSN:

1015-3918