Evaluation of Femur Length in Rats Using Three Different Methods and the Reliability of the Anthropometric Measurement Method

Ahmet Emrah Açan; Burcu Aslantekin; Oğuzhan Selçuk Aldemir; Emrah Özcan; Sinan Saraçlı; Özgür Bulmuş; Aslı Karakılıç; Gülay Turan

Makale detayları

Evaluation of Femur Length in Rats Using Three Different Methods and the Reliability of the Anthropometric Measurement Method

Yazarlar:

Ahmet Emrah Açan | Burcu Aslantekin | Oğuzhan Selçuk Aldemir | Emrah Özcan | Sinan Saraçlı | Özgür Bulmuş | Aslı Karakılıç | Gülay Turan

Yayıncı:

Balıkesir University

Ana Başlıklar:

Cerrahi (Diğer)

Dergi:

Balıkesir Sağlık Bilimleri Dergisi Vol.14 Issue 2, pp.514-522

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357567

pISSN:

2146-9601

Anahtar kelimeler:

Özet:

 Objective: The skeletal system evaluated in rodent models plays a crucial role in various experimental studies, including bone development, fracture healing, biomechanical testing, osteoporosis, drug efficacy, and safety. This study aims to assess the applicability of the anthropometric method, which is widely used in humans, for measuring femur length in rats. Additionally, the reliability of the anthropometric method was compared with dissection and radiological methods. Materials and Methods: A total of 66 male Wistar Albino rats 1416 weeks old were used. The right femur length was measured using three different methods: anthropometric measurement, digital caliper measurement after dissection, and radiological measurement via micro-CT. The obtained data were analyzed, and the agreement between methods was evaluated using the Bland-Altman test. Results: The mean femur length was measured as 37.57 mm anthropometrically, 33.55 mm using a digital caliper post-dissection, and 33.84 mm using micro-CT. The intraclass correlation coefficient ICC values at a 90 confidence interval were as follows: 0.987 0.9790.992 between the dissection and radiological groups, 0.709 0.5640.812 between the anthropometric and dissection groups, and 0.713 0.5690.814 between the anthropometric and radiological groups. The anthropometric method showed a significant agreement with both dissection and radiological methods, with a difference of approximately 4 mm. Conclusion: The anthropometric method has been demonstrated to be a reliable, easy-to-use, non-invasive, cost-effective, and time-saving alternative for measuring femur length in rats. Since this method allows for repeated measurements on the same animal, it can reduce the number of animals used in studies, thereby promoting more ethical and efficient research in bone studies.

 Objective: The skeletal system evaluated in rodent models plays a crucial role in various experimental studies, including bone development, fracture healing, biomechanical testing, osteoporosis, drug efficacy, and safety. This study aims to assess the applicability of the anthropometric method, which is widely used in humans, for measuring femur length in rats. Additionally, the reliability of the anthropometric method was compared with dissection and radiological methods. Materials and Methods: A total of 66 male Wistar Albino rats 1416 weeks old were used. The right femur length was measured using three different methods: anthropometric measurement, digital caliper measurement after dissection, and radiological measurement via micro-CT. The obtained data were analyzed, and the agreement between methods was evaluated using the Bland-Altman test. Results: The mean femur length was measured as 37.57 mm anthropometrically, 33.55 mm using a digital caliper post-dissection, and 33.84 mm using micro-CT. The intraclass correlation coefficient ICC values at a 90 confidence interval were as follows: 0.987 0.9790.992 between the dissection and radiological groups, 0.709 0.5640.812 between the anthropometric and dissection groups, and 0.713 0.5690.814 between the anthropometric and radiological groups. The anthropometric method showed a significant agreement with both dissection and radiological methods, with a difference of approximately 4 mm. Conclusion: The anthropometric method has been demonstrated to be a reliable, easy-to-use, non-invasive, cost-effective, and time-saving alternative for measuring femur length in rats. Since this method allows for repeated measurements on the same animal, it can reduce the number of animals used in studies, thereby promoting more ethical and efficient research in bone studies.

 Objective: The skeletal system evaluated in rodent models plays a crucial role in various experimental studies, including bone development, fracture healing, biomechanical testing, osteoporosis, drug efficacy, and safety. This study aims to assess the applicability of the anthropometric method, which is widely used in humans, for measuring femur length in rats. Additionally, the reliability of the anthropometric method was compared with dissection and radiological methods. Materials and Methods: A total of 66 male Wistar Albino rats 1416 weeks old were used. The right femur length was measured using three different methods: anthropometric measurement, digital caliper measurement after dissection, and radiological measurement via micro-CT. The obtained data were analyzed, and the agreement between methods was evaluated using the Bland-Altman test. Results: The mean femur length was measured as 37.57 mm anthropometrically, 33.55 mm using a digital caliper post-dissection, and 33.84 mm using micro-CT. The intraclass correlation coefficient ICC values at a 90 confidence interval were as follows: 0.987 0.9790.992 between the dissection and radiological groups, 0.709 0.5640.812 between the anthropometric and dissection groups, and 0.713 0.5690.814 between the anthropometric and radiological groups. The anthropometric method showed a significant agreement with both dissection and radiological methods, with a difference of approximately 4 mm. Conclusion: The anthropometric method has been demonstrated to be a reliable, easy-to-use, non-invasive, cost-effective, and time-saving alternative for measuring femur length in rats. Since this method allows for repeated measurements on the same animal, it can reduce the number of animals used in studies, thereby promoting more ethical and efficient research in bone studies.

 Amaç: Kemirgen modellerinde değerlendirilen iskelet sistemi; kemik gelişimi, kırık iyileşmesi, biyomekanik testler, osteoporoz, ilaç etkinliği ve güvenliği gibi birçok deneyde önemli yer tutmaktadır. Bu çalışmada, sıçanlarda en çok değerlendirilen kemiklerden olan femurun uzunluğunu ölçmek için insanlarda yaygın olarak kullanılan antropometrik yöntemin sıçanlardaki uygulanabilirliği, bunun yanında antropometrik yöntemin güvenilirliğinin diseksiyon ve radyolojik yöntemler ile karşılaştırılması amaçlanmıştır. Gereç ve Yöntem: 66 adet erkek Wistar Albino sıçan kullanılmıştır ve sağ femur uzunluğu antropometrik olarak, diseksiyon sonrası dijital kumpas ile ve mikroCT ile radyolojik olarak ölçülmüştür. Elde edilen veriler analiz edilmiş ve Bland-Altman testi ile yöntemler arasındaki uyum değerlendirilmiştir. Bulgular: Femur uzunluğu; antropometrik olarak 37.57 mm, diseksiyon sonrası dijital kumpas ile 33.55 mm, mikroCT ile 33.84 mm bulunmuştur. 90 güven aralığında ICC katsayıları; diseksiyon ve radyolojik grup arasında 0.987 0.979-0.992, antropometrik ve diseksiyon grup arasında 0.709 0.564-0.812, antropometrik ve radyolojik grup arasında 0.713 0.569-0.814 şeklindedir. Antropometrik yöntem, diseksiyon sonrası ve radyolojik yöntemlerle arasında 4 mmlik farkla önemli derecede uyumlu olarak bulunmuştur. Sonuç: Sonuç olarak sıçanlarda femur uzunluğunu ölçmek için antropometrik yöntem, güvenilir, kolay uygulanabilir, invaziv olmayan, maliyet ve zaman tasarrufu sağlayan alternatif bir yöntem olduğu ortaya koyulmuştur. Bu yöntem, kullanılan hayvan sayısını azaltıp tek bir hayvanın tekrar tekrar değerlendirilmesine olanak sağladığından kemik çalışmalarında daha etik ve daha verimli çalışmaların yapılmasına imkân sağlayabilir.

Makale detayları

Evaluation of Femur Length in Rats Using Three Different Methods and the Reliability of the Anthropometric Measurement Method

Yazarlar:

Ahmet Emrah Açan | Burcu Aslantekin | Oğuzhan Selçuk Aldemir | Emrah Özcan | Sinan Saraçlı | Özgür Bulmuş | Aslı Karakılıç | Gülay Turan

Yayıncı:

Balıkesir University

Ana Başlıklar:

Cerrahi (Diğer)

Dergi:

Balıkesir Sağlık Bilimleri Dergisi Vol.14 Issue 2, pp.514-522

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357567

pISSN:

2146-9601

Keywords: Femur length

rat

anthropometry

dissection

micro-CT.Anahtar kelimeler: Femur uzunluğu

sıçan

antropometri

diseksiyon

mikroCT.

Sayı

Makale detayları

Yazarlar: Ahmet Emrah Açan | Burcu Aslantekin | Oğuzhan Selçuk Aldemir | Emrah Özcan | Sinan Saraçlı | Özgür Bulmuş | Aslı Karakılıç | Gülay Turan

Yayıncı: Balıkesir University

Ana Başlıklar: Cerrahi (Diğer)

Dergi: Balıkesir Sağlık Bilimleri Dergisi Vol.14 Issue 2, pp.514-522

Belge Tipi: Makale

Yayın Tarihi: 8/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001357567

pISSN: 2146-9601

Sayı

Yazarlar:

Ahmet Emrah Açan | Burcu Aslantekin | Oğuzhan Selçuk Aldemir | Emrah Özcan | Sinan Saraçlı | Özgür Bulmuş | Aslı Karakılıç | Gülay Turan

Yayıncı:

Balıkesir University

Ana Başlıklar:

Cerrahi (Diğer)

Dergi:

Balıkesir Sağlık Bilimleri Dergisi Vol.14 Issue 2, pp.514-522

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357567

pISSN:

2146-9601