The Effect of Necrostatin -1 and Enoxaparin Molecules on Random Pattern Flap Viability

Ömer Faruk Çınar; Nagihan Bilal; Emine Kılınç; Muhammed Seyithanoğlu; İsrafil Orhan; Muhammed Gazi Yıldız

Makale detayları

The Effect of Necrostatin -1 and Enoxaparin Molecules on Random Pattern Flap Viability

Yazarlar:

Ömer Faruk Çınar | Nagihan Bilal | Emine Kılınç | Muhammed Seyithanoğlu | İsrafil Orhan | Muhammed Gazi Yıldız

Yayıncı:

Karyay Karadeniz Yayımcılık

Ana Başlıklar:

Cerrahi (Diğer)

Dergi:

Black Sea Journal of Health Science Vol.7 Issue 2, pp.98-105

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001311729

pISSN:

2619-9041

Anahtar kelimeler:

Özet:

 Distal flap necrosis is seen more often in random pattern flaps and is an important complication that shortens the flap length. There has been much research many drugs and molecules in an effort to prevent this complication. The aim of this study was to investigate the efficacy of necrostatin-1 and enoxaparin molecules in preventing distal flap necrosis and increasing flap viability in a random pattern flap model created in rats. A total of 32 Wistar albino female rats, each weighing 300-350 gr were separated into 4 groups. All the animals underwent an operation to create a 39 cm caudal-based Mcfarlane flap. The treatments defined for each group were applied. Full layer tisssue samples 11 cm2 were taken from all the flaps and stored until histopathological and immunohistochemical examination, the parameters of inflammation, capillary proliferation, necrosis, fibroblast proliferation and fibrosis were compared histopathologically. In the necrostatin-1 group, the inflammation, necrosis and fibrosis scores were observed to be lower and the capillary proliferation and fibroblast proliferation scores were higher. In the enoxaparin group, the fibroblast proliferation and capillary proliferation scores were higher. The receptor interacting protein kinase-1 immunohistochemical staining results showed statistically significantly less staining in the necrostatin-1 group compared to the other groups. The results of this study suggest that necrostatin molecule has important therapeutic potential in increasing flap viability in the random pattern flap model, considering the percentage of flap necrosis, and the immunohistochemical and histopathological data. The flap necrosis percentage and histochemical parameters of the enoxaparin molecule demonstrate that the effects on flap viability are limited.

 Distal flap necrosis is seen more often in random pattern flaps and is an important complication that shortens the flap length. There has been much research many drugs and molecules in an effort to prevent this complication. The aim of this study was to investigate the efficacy of necrostatin-1 and enoxaparin molecules in preventing distal flap necrosis and increasing flap viability in a random pattern flap model created in rats. A total of 32 Wistar albino female rats, each weighing 300-350 gr were separated into 4 groups. All the animals underwent an operation to create a 39 cm caudal-based Mcfarlane flap. The treatments defined for each group were applied. Full layer tisssue samples 11 cm2 were taken from all the flaps and stored until histopathological and immunohistochemical examination, the parameters of inflammation, capillary proliferation, necrosis, fibroblast proliferation and fibrosis were compared histopathologically. In the necrostatin-1 group, the inflammation, necrosis and fibrosis scores were observed to be lower and the capillary proliferation and fibroblast proliferation scores were higher. In the enoxaparin group, the fibroblast proliferation and capillary proliferation scores were higher. The receptor interacting protein kinase-1 immunohistochemical staining results showed statistically significantly less staining in the necrostatin-1 group compared to the other groups. The results of this study suggest that necrostatin molecule has important therapeutic potential in increasing flap viability in the random pattern flap model, considering the percentage of flap necrosis, and the immunohistochemical and histopathological data. The flap necrosis percentage and histochemical parameters of the enoxaparin molecule demonstrate that the effects on flap viability are limited.

 Distal flap necrosis is seen more often in random pattern flaps and is an important complication that shortens the flap length. There has been much research many drugs and molecules in an effort to prevent this complication. The aim of this study was to investigate the efficacy of necrostatin-1 and enoxaparin molecules in preventing distal flap necrosis and increasing flap viability in a random pattern flap model created in rats. A total of 32 Wistar albino female rats, each weighing 300-350 gr were separated into 4 groups. All the animals underwent an operation to create a 39 cm caudal-based Mcfarlane flap. The treatments defined for each group were applied. Full layer tisssue samples 11 cm2 were taken from all the flaps and stored until histopathological and immunohistochemical examination, the parameters of inflammation, capillary proliferation, necrosis, fibroblast proliferation and fibrosis were compared histopathologically. In the necrostatin-1 group, the inflammation, necrosis and fibrosis scores were observed to be lower and the capillary proliferation and fibroblast proliferation scores were higher. In the enoxaparin group, the fibroblast proliferation and capillary proliferation scores were higher. The receptor interacting protein kinase-1 immunohistochemical staining results showed statistically significantly less staining in the necrostatin-1 group compared to the other groups. The results of this study suggest that necrostatin molecule has important therapeutic potential in increasing flap viability in the random pattern flap model, considering the percentage of flap necrosis, and the immunohistochemical and histopathological data. The flap necrosis percentage and histochemical parameters of the enoxaparin molecule demonstrate that the effects on flap viability are limited.

 Distal flap necrosis is seen more often in random pattern flaps and is an important complication that shortens the flap length. There has been much research many drugs and molecules in an effort to prevent this complication. The aim of this study was to investigate the efficacy of necrostatin-1 and enoxaparin molecules in preventing distal flap necrosis and increasing flap viability in a random pattern flap model created in rats. A total of 32 Wistar albino female rats, each weighing 300-350 gr were separated into 4 groups. All the animals underwent an operation to create a 39 cm caudal-based Mcfarlane flap. The treatments defined for each group were applied. Full layer tisssue samples 11 cm2 were taken from all the flaps and stored until histopathological and immunohistochemical examination, the parameters of inflammation, capillary proliferation, necrosis, fibroblast proliferation and fibrosis were compared histopathologically. In the necrostatin-1 group, the inflammation, necrosis and fibrosis scores were observed to be lower and the capillary proliferation and fibroblast proliferation scores were higher. In the enoxaparin group, the fibroblast proliferation and capillary proliferation scores were higher. The receptor interacting protein kinase-1 immunohistochemical staining results showed statistically significantly less staining in the necrostatin-1 group compared to the other groups. The results of this study suggest that necrostatin molecule has important therapeutic potential in increasing flap viability in the random pattern flap model, considering the percentage of flap necrosis, and the immunohistochemical and histopathological data. The flap necrosis percentage and histochemical parameters of the enoxaparin molecule demonstrate that the effects on flap viability are limited.

Makale detayları

The Effect of Necrostatin -1 and Enoxaparin Molecules on Random Pattern Flap Viability

Yazarlar:

Ömer Faruk Çınar | Nagihan Bilal | Emine Kılınç | Muhammed Seyithanoğlu | İsrafil Orhan | Muhammed Gazi Yıldız

Yayıncı:

Karyay Karadeniz Yayımcılık

Ana Başlıklar:

Cerrahi (Diğer)

Dergi:

Black Sea Journal of Health Science Vol.7 Issue 2, pp.98-105

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001311729

pISSN:

2619-9041

Necrostatin-1

Enoxaparin

Rat

Flap necrosis

Flap viabilityNecrostatin-1

Enoxaparin

Rat

Flap necrosis

Flap viability

Sayı

Makale detayları

Yazarlar: Ömer Faruk Çınar | Nagihan Bilal | Emine Kılınç | Muhammed Seyithanoğlu | İsrafil Orhan | Muhammed Gazi Yıldız

Yayıncı: Karyay Karadeniz Yayımcılık

Ana Başlıklar: Cerrahi (Diğer)

Dergi: Black Sea Journal of Health Science Vol.7 Issue 2, pp.98-105

Belge Tipi: Makale

Yayın Tarihi: 1/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001311729

pISSN: 2619-9041

Sayı

Yazarlar:

Ömer Faruk Çınar | Nagihan Bilal | Emine Kılınç | Muhammed Seyithanoğlu | İsrafil Orhan | Muhammed Gazi Yıldız

Yayıncı:

Karyay Karadeniz Yayımcılık

Ana Başlıklar:

Cerrahi (Diğer)

Dergi:

Black Sea Journal of Health Science Vol.7 Issue 2, pp.98-105

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001311729

pISSN:

2619-9041