Molecular characterization of Escherichia coli isolated from raw cow milk samples collected from district Bahawalpur, Pakistan

Bushra PARVEEN; Yasmeen JUNEJO; Muhammad SAFDAR; Mehmet ÖZASLAN

Makale detayları

Molecular characterization of Escherichia coli isolated from raw cow milk samples collected from district Bahawalpur, Pakistan

Yazarlar:

Bushra PARVEEN | Yasmeen JUNEJO | Muhammad SAFDAR | Mehmet ÖZASLAN

Yayıncı:

Mehmet ÖZASLAN

Ana Başlıklar:

Gıda teknolojisi

Dergi:

Zeugma Biological Science 2021 Vol.2 Issue 2, pp.1-13

Belge Tipi:

Makale

Yayın Tarihi:

4/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000654072

Sıklık:

Üç Yıllık

pISSN:

2757-5055

Özet:

 

The present study was structured for molecular detection and characterization of Escherichia. coli (E. coli) isolated from cow’s raw milk samples. A total of 100 milk samples were collected in a way that 10 samples each were collected from markets of Jamalpur, Hasilpur, Khairpur, Qaimpur, Lal Sohanra, Lal Sohanra Park, Yazman Mandi, Rajkan, Ahmedpur East and Uch Sharif, district Bahawalpur, Pakistan. Milk samples were then cultured on different culture media for bacterial isolation. Identification of bacteria was done through Gram’s staining, properties of bacterial culture on different selective media and biochemical tests including catalase and coagulase tests. Final identification was performed through PCR and resolution of PCR products by gel electrophoresis. Out of 100 samples, 43 samples were found to be contaminated with E. coli. E. coli isolates were then amplified by 16S rRNA gene-based PCR. Antimicrobial sensitivity test was also performed to confirm the susceptibility of E. coli to different antibiotics. Results of the antimicrobial sensitivity test showed that the E. coli isolates were resistant to amoxicillin and erythromycin but sensitive to azithromycin, ciprofloxacin, gentamicin, norfloxacin and streptomycin.

Makale detayları

Molecular characterization of Escherichia coli isolated from raw cow milk samples collected from district Bahawalpur, Pakistan

Yazarlar: Bushra PARVEEN | Yasmeen JUNEJO | Muhammad SAFDAR | Mehmet ÖZASLAN

Yayıncı: Mehmet ÖZASLAN

Ana Başlıklar: Gıda teknolojisi

Dergi: Zeugma Biological Science 2021 Vol.2 Issue 2, pp.1-13

Belge Tipi: Makale

Yayın Tarihi: 4/2021

Belge Dili: İngilizce

Document ID (Org No.): 2000654072

Sıklık: Üç Yıllık

pISSN: 2757-5055

Sayı

Yazarlar:

Bushra PARVEEN | Yasmeen JUNEJO | Muhammad SAFDAR | Mehmet ÖZASLAN

Yayıncı:

Mehmet ÖZASLAN

Ana Başlıklar:

Gıda teknolojisi

Dergi:

Zeugma Biological Science 2021 Vol.2 Issue 2, pp.1-13

Belge Tipi:

Makale

Yayın Tarihi:

4/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000654072

Sıklık:

Üç Yıllık

pISSN:

2757-5055