The performance characteristics of low-cost and custom-designed dual beam optical tweezer

Nur Çobanoğlu; Aziz Kolkıran

Makale detayları

The performance characteristics of low-cost and custom-designed dual beam optical tweezer

Yazarlar:

Nur Çobanoğlu | Aziz Kolkıran

Yayıncı:

Gumushane University

Ana Başlıklar:

Klasik ve Fiziksel Optik

Dergi:

Gümüşhane Üniversitesi Fen Bilimleri Dergisi Vol.14 Issue 3, pp.851-863

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001326540

pISSN:

2146-538X

Anahtar kelimeler:

Özet:

 In this work is a low-cost and custom-designed optical tweezers using two He-Ne lasers λ632.8 nm positioned in parallel is developed to trap and manipulate micron-sized particles. Polystyrene beads in water and yeast cells in yogurt culture were used as samples. To determine the characteristics of these optical tweezers, the stiffness and trapping force were determined using the Brownian Motion method for Laser 1, Laser 2 and the case where both lasers were used simultaneously. Small adjustments of the optical components affected the trapping force, resulting in a lower trapping force for Laser 2. In addition, the viscosity of the scattering medium has a significant effect on the trapping force; the higher viscosity of the yogurt culture prevents the capture of yeast cells with the simultaneous use of both lasers. The use of two lasers in parallel allows one laser to be used to capture and move particles while the other is used to capture multiple particles. This custom-designed optical tweezer, whose performance was investigated for different samples, has the potential to be applied in life science fields such as viscosity measurements, intracellular investigations, food engineering and drug delivery systems.

 In this work is a low-cost and custom-designed optical tweezers using two He-Ne lasers λ632.8 nm positioned in parallel is developed to trap and manipulate micron-sized particles. Polystyrene beads in water and yeast cells in yogurt culture were used as samples. To determine the characteristics of these optical tweezers, the stiffness and trapping force were determined using the Brownian Motion method for Laser 1, Laser 2 and the case where both lasers were used simultaneously. Small adjustments of the optical components affected the trapping force, resulting in a lower trapping force for Laser 2. In addition, the viscosity of the scattering medium has a significant effect on the trapping force; the higher viscosity of the yogurt culture prevents the capture of yeast cells with the simultaneous use of both lasers. The use of two lasers in parallel allows one laser to be used to capture and move particles while the other is used to capture multiple particles. This custom-designed optical tweezer, whose performance was investigated for different samples, has the potential to be applied in life science fields such as viscosity measurements, intracellular investigations, food engineering and drug delivery systems.

 In this work is a low-cost and custom-designed optical tweezers using two He-Ne lasers λ632.8 nm positioned in parallel is developed to trap and manipulate micron-sized particles. Polystyrene beads in water and yeast cells in yogurt culture were used as samples. To determine the characteristics of these optical tweezers, the stiffness and trapping force were determined using the Brownian Motion method for Laser 1, Laser 2 and the case where both lasers were used simultaneously. Small adjustments of the optical components affected the trapping force, resulting in a lower trapping force for Laser 2. In addition, the viscosity of the scattering medium has a significant effect on the trapping force; the higher viscosity of the yogurt culture prevents the capture of yeast cells with the simultaneous use of both lasers. The use of two lasers in parallel allows one laser to be used to capture and move particles while the other is used to capture multiple particles. This custom-designed optical tweezer, whose performance was investigated for different samples, has the potential to be applied in life science fields such as viscosity measurements, intracellular investigations, food engineering and drug delivery systems.

 Bu çalışmada, mikron boyutundaki partikülleri yakalamak ve manipüle etmek için paralel olarak konumlandırılmış iki adet He-Ne lazer λ632.8 nm kullanan düşük bütçeli ve özel tasarlanmış bir optik cımbız geliştirilmiştir. Bu kapsamda örnek olarak sudaki polistiren boncuklar ve yoğurt kültüründeki maya hücreleri kullanılmıştır. Bu optik cımbızın özelliklerini belirlemek için sertlik ve yakalama kuvveti, Lazer 1, Lazer 2 ve her iki lazerin aynı anda kullanıldığı durum için Brownian Hareketi yöntemi kullanılarak belirlenmiştir. Optik bileşenlerde yapılan küçük ayarlamalar yakalama kuvvetini etkileyerek Lazer 2nin yakalama kuvvetinin daha düşük olmasına neden olmuştur. Ek olarak, saçılma ortamının viskozitesinin yakalama kuvveti üzerinde önemli bir etkisi vardır; yoğurt kültürünün daha yüksek viskozitesi, maya hücrelerinin her iki lazerin aynı anda kullanılmasıyla yakalanmasını önlemektedir. İki lazerin paralel olarak kullanılması, bir lazerin parçacıkları yakalamak ve hareket ettirmek için kullanılırken diğerinin birden fazla parçacığı yakalamak için kullanılmasını sağlamaktadır. Farklı çalışma ortamlarında performansı incelenen bu özel tasarım optik cımbız, viskozite ölçümleri, hücre içi incelemeler, gıda mühendisliği ve ilaç dağıtım sistemleri gibi yaşambilim alanlarında uygulanma potansiyeline sahiptir.

Makale detayları

The performance characteristics of low-cost and custom-designed dual beam optical tweezer

Yazarlar:

Nur Çobanoğlu | Aziz Kolkıran

Yayıncı:

Gumushane University

Ana Başlıklar:

Klasik ve Fiziksel Optik

Dergi:

Gümüşhane Üniversitesi Fen Bilimleri Dergisi Vol.14 Issue 3, pp.851-863

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001326540

pISSN:

2146-538X

He-Ne laser

Optical tweezer

Optical trappingHe-Ne lazer

Optik cımbız

Optik yakalama

Sayı

Makale detayları

The performance characteristics of low-cost and custom-designed dual beam optical tweezer

Yazarlar: Nur Çobanoğlu | Aziz Kolkıran

Yayıncı: Gumushane University

Ana Başlıklar: Klasik ve Fiziksel Optik

Dergi: Gümüşhane Üniversitesi Fen Bilimleri Dergisi Vol.14 Issue 3, pp.851-863

Belge Tipi: Makale

Yayın Tarihi: 9/2024

Belge Dili: Türkçe

Document ID (Org No.): 2001326540

pISSN: 2146-538X

He-Ne laser

Optical tweezer

Optical trappingHe-Ne lazer

Optik cımbız

Optik yakalama

Sayı

Yazarlar:

Nur Çobanoğlu | Aziz Kolkıran

Yayıncı:

Gumushane University

Ana Başlıklar:

Klasik ve Fiziksel Optik

Dergi:

Gümüşhane Üniversitesi Fen Bilimleri Dergisi Vol.14 Issue 3, pp.851-863

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001326540

pISSN:

2146-538X