Effect of sodium nitroprusside on micropropagation and biochemical parameters of CAB-6P and Gisela 6 cherry rootstocks

VIRGINIA SARROPOULOU; KORTESSA DIMASSI-THERIOU; IOANNIS THERIOS

Makale detayları

Effect of sodium nitroprusside on micropropagation and biochemical parameters of CAB-6P and Gisela 6 cherry rootstocks

Yazarlar:

VIRGINIA SARROPOULOU | KORTESSA DIMASSI-THERIOU | IOANNIS THERIOS

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 4, pp.595-610

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811888

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

The effects of sodium nitroprusside (SNP) on micropropagation of CAB-6P and Gisela 6 cherry rootstocks were investigated. Shoot multiplication of CAB-6P was reduced by adding 4.4 μμ\muM 6-benzylaminopurine (BA) and SNP. Gisela 6 microcuttings treated with BA + 30 μμ\muM SNP gave the highest average shoot number (4.15) and multiplication percentage (100%). In CAB-6P, αα\alpha-naphthaleneacetic acid (NAA) + 30 μμ\muM SNP led to the maximum root number (13.79) and fresh weight (0.336 g); average root length (82 mm) and rooting percentage (95.45%) were greatest after adding 40 and 10 μμ\muM SNP + NAA, respectively. In Gisela 6, NAA + 40 μμ\muM SNP resulted in the highest root number (9.75) and fresh weight (0.238 g), while NAA + 30 μμ\muM SNP enabled maximum root length (30.77 mm). Rooting percentage was greatest (84.62%) when Gisela 6 microcuttings were treated only with NAA. Application of SNP alone in CAB-6P reduced leaf chlorophyll, carbohydrate, and pr?line levels. In contrast, 30?50 μμ\muM SNP increased leaf chlorophyll levels of Gisela 6. SNP augmented leaf carbohydrate and proline content, but it diminished root carbohydrate content. In BA + SNP or NAA + SNP treatments, changes in biochemical constituents were dependent on SNP concentrations and genotypes.

 

The effects of sodium nitroprusside (SNP) on micropropagation of CAB-6P and Gisela 6 cherry rootstocks were investigated. Shoot multiplication of CAB-6P was reduced by adding 4.4 μμ\muM 6-benzylaminopurine (BA) and SNP. Gisela 6 microcuttings treated with BA + 30 μμ\muM SNP gave the highest average shoot number (4.15) and multiplication percentage (100%). In CAB-6P, αα\alpha-naphthaleneacetic acid (NAA) + 30 μμ\muM SNP led to the maximum root number (13.79) and fresh weight (0.336 g); average root length (82 mm) and rooting percentage (95.45%) were greatest after adding 40 and 10 μμ\muM SNP + NAA, respectively. In Gisela 6, NAA + 40 μμ\muM SNP resulted in the highest root number (9.75) and fresh weight (0.238 g), while NAA + 30 μμ\muM SNP enabled maximum root length (30.77 mm). Rooting percentage was greatest (84.62%) when Gisela 6 microcuttings were treated only with NAA. Application of SNP alone in CAB-6P reduced leaf chlorophyll, carbohydrate, and pr?line levels. In contrast, 30?50 μμ\muM SNP increased leaf chlorophyll levels of Gisela 6. SNP augmented leaf carbohydrate and proline content, but it diminished root carbohydrate content. In BA + SNP or NAA + SNP treatments, changes in biochemical constituents were dependent on SNP concentrations and genotypes.

Makale detayları

Effect of sodium nitroprusside on micropropagation and biochemical parameters of CAB-6P and Gisela 6 cherry rootstocks

Yazarlar:

VIRGINIA SARROPOULOU | KORTESSA DIMASSI-THERIOU | IOANNIS THERIOS

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 4, pp.595-610

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811888

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Auxin

carbohydrate level

chlorophyll content

cytokinin

endogenous proline concentration

nitric oxide

plant tissue culture

rhizogenesis

shoot proliferation

Sayı

Makale detayları

Yazarlar: VIRGINIA SARROPOULOU | KORTESSA DIMASSI-THERIOU | IOANNIS THERIOS

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2015 Vol.39 Issue 4, pp.595-610

Belge Tipi: Makale

Yayın Tarihi: 8/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000811888

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

VIRGINIA SARROPOULOU | KORTESSA DIMASSI-THERIOU | IOANNIS THERIOS

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 4, pp.595-610

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811888

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152