Simultaneous Determination of Flurbiprofen and Thiocolchicoside in Pharmaceutical Preparations by a Validated HPLC Method

Ebru Türköz Acar

Makale detayları

Simultaneous Determination of Flurbiprofen and Thiocolchicoside in Pharmaceutical Preparations by a Validated HPLC Method

Yazarlar:

Ebru Türköz Acar

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Analytical Chemistry | Pharmaceutical Chemistry

Dergi:

Journal of Research in Pharmacy Vol.26 Issue 3, pp.663-674

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346863

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 In this study, a new high performance liquid chromatography HPLC analysis method was developed for the simultaneous determination of flurbiprofen F and thiocolchicoside T in pharmaceutical products. A C18 column Agilent Poroshell 120 EC-C18 was used as the stationary phase. Elution of the analytes was achieved by using gradient elution system. Mobile phase A was an acetate buffer 100 mM, pH 4.8 and mobile phase B was acetonitrile. The flow rate for the method developed was 0.5 mlmin. The temperature of the column compartment was set at 40C and the injection volume was 5 μl. Monitoring of the analytes was carried out at 248 nm. The method was found linear in the concentration range between 5-100 ppm. The correlation coefficient value was calculated as 0.999 for F and 0.999 for T. The method was found suitable in terms of accuracy, precision, specificity, linearity, and robustness ruggedness. Furthermore, it was applied successfully for the analysis of commercial tablets and gel samples that contains F and T without any time-consuming pre-procedure. The recovery values for Fand T were found as 99.5 and 97.0 in tablet formulations and 98.6 and 99.3 in gel formulations, respectively. All results were acceptable and this confirmed that the method is suitable for its intended use in routine quality control and an assay of drugs.

 In this study, a new high performance liquid chromatography HPLC analysis method was developed for the simultaneous determination of flurbiprofen F and thiocolchicoside T in pharmaceutical products. A C18 column Agilent Poroshell 120 EC-C18 was used as the stationary phase. Elution of the analytes was achieved by using gradient elution system. Mobile phase A was an acetate buffer 100 mM, pH 4.8 and mobile phase B was acetonitrile. The flow rate for the method developed was 0.5 mlmin. The temperature of the column compartment was set at 40C and the injection volume was 5 μl. Monitoring of the analytes was carried out at 248 nm. The method was found linear in the concentration range between 5-100 ppm. The correlation coefficient value was calculated as 0.999 for F and 0.999 for T. The method was found suitable in terms of accuracy, precision, specificity, linearity, and robustness ruggedness. Furthermore, it was applied successfully for the analysis of commercial tablets and gel samples that contains F and T without any time-consuming pre-procedure. The recovery values for Fand T were found as 99.5 and 97.0 in tablet formulations and 98.6 and 99.3 in gel formulations, respectively. All results were acceptable and this confirmed that the method is suitable for its intended use in routine quality control and an assay of drugs.

 In this study, a new high performance liquid chromatography HPLC analysis method was developed for the simultaneous determination of flurbiprofen F and thiocolchicoside T in pharmaceutical products. A C18 column Agilent Poroshell 120 EC-C18 was used as the stationary phase. Elution of the analytes was achieved by using gradient elution system. Mobile phase A was an acetate buffer 100 mM, pH 4.8 and mobile phase B was acetonitrile. The flow rate for the method developed was 0.5 mlmin. The temperature of the column compartment was set at 40C and the injection volume was 5 μl. Monitoring of the analytes was carried out at 248 nm. The method was found linear in the concentration range between 5-100 ppm. The correlation coefficient value was calculated as 0.999 for F and 0.999 for T. The method was found suitable in terms of accuracy, precision, specificity, linearity, and robustness ruggedness. Furthermore, it was applied successfully for the analysis of commercial tablets and gel samples that contains F and T without any time-consuming pre-procedure. The recovery values for Fand T were found as 99.5 and 97.0 in tablet formulations and 98.6 and 99.3 in gel formulations, respectively. All results were acceptable and this confirmed that the method is suitable for its intended use in routine quality control and an assay of drugs.

 In this study, a new high performance liquid chromatography HPLC analysis method was developed for the simultaneous determination of flurbiprofen F and thiocolchicoside T in pharmaceutical products. A C18 column Agilent Poroshell 120 EC-C18 was used as the stationary phase. Elution of the analytes was achieved by using gradient elution system. Mobile phase A was an acetate buffer 100 mM, pH 4.8 and mobile phase B was acetonitrile. The flow rate for the method developed was 0.5 mlmin. The temperature of the column compartment was set at 40C and the injection volume was 5 μl. Monitoring of the analytes was carried out at 248 nm. The method was found linear in the concentration range between 5-100 ppm. The correlation coefficient value was calculated as 0.999 for F and 0.999 for T. The method was found suitable in terms of accuracy, precision, specificity, linearity, and robustness ruggedness. Furthermore, it was applied successfully for the analysis of commercial tablets and gel samples that contains F and T without any time-consuming pre-procedure. The recovery values for Fand T were found as 99.5 and 97.0 in tablet formulations and 98.6 and 99.3 in gel formulations, respectively. All results were acceptable and this confirmed that the method is suitable for its intended use in routine quality control and an assay of drugs.

Makale detayları

Simultaneous Determination of Flurbiprofen and Thiocolchicoside in Pharmaceutical Preparations by a Validated HPLC Method

Yazarlar:

Ebru Türköz Acar

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Analytical Chemistry | Pharmaceutical Chemistry

Dergi:

Journal of Research in Pharmacy Vol.26 Issue 3, pp.663-674

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346863

pISSN:

2630-6344

Flurbiprofen

Thiocolchicosoide

HPLC

analysis

validation

commercial dosage forms

Sayı

Makale detayları

Simultaneous Determination of Flurbiprofen and Thiocolchicoside in Pharmaceutical Preparations by a Validated HPLC Method

Yazarlar: Ebru Türköz Acar

Yayıncı: Marmara University

Ana Başlıklar: Pharmaceutical Analytical Chemistry | Pharmaceutical Chemistry

Dergi: Journal of Research in Pharmacy Vol.26 Issue 3, pp.663-674

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001346863

pISSN: 2630-6344

Flurbiprofen

Thiocolchicosoide

HPLC

analysis

validation

commercial dosage forms

Sayı

Yazarlar:

Ebru Türköz Acar

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Analytical Chemistry | Pharmaceutical Chemistry

Dergi:

Journal of Research in Pharmacy Vol.26 Issue 3, pp.663-674

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346863

pISSN:

2630-6344