Effect of ascorbic acid on collagen and some inflammatory mediators in rats

Sibel Işın; Nuray Yazıhan; Burcu Kesikli; Derya Biriken; Sevginur Akdaş; Ferda Topal Celikkan; Batuhan Bakırarar; Pelin Arıbal-ayral

Makale detayları

Effect of ascorbic acid on collagen and some inflammatory mediators in rats

Yazarlar:

Sibel Işın | Nuray Yazıhan | Burcu Kesikli | Derya Biriken | Sevginur Akdaş | Ferda Topal Celikkan | Batuhan Bakırarar | Pelin Arıbal-ayral

Yayıncı:

Ankara University

Ana Başlıklar:

Veterinary Medicine | Veterinary Surgery

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.70 Issue 3, pp.303-308

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001292292

pISSN:

1300-0861

Anahtar kelimeler:

Özet:

 Musculoskeletal injuries as a kind of trauma that the human body is exposed to, adversely affect the quality of life and workforce of individuals due to restriction of movement function. This study aimed to evaluate the effects of dose-dependent ascorbic acid AA administration on the repair process after gastrocnemius muscle injury in rats. In this study, 5-month-old 66 male Wistar Albino rats were used and rats were randomly divided into 6 groups of 11 each control, muscle injury, healthy with 5 mg10 mgkgday AA-treated group, injury with 5 mg10 mgkgday AA-treated group. A linear incision was made in the gastrocnemius muscle of thirty-three animals included in the muscle injury groups. AA 5-10 mgkgday was administered to the four groups intraperitoneally just after surgery once a day. Animals were sacrificed twenty-one days later. Blood and tissue samples were used for cytokine, collagen, and histological measurements. It was found that a dose of 5 mgkgday AA administration reduced serum IL-6 and muscle tissue TNF-α levels, and increased liver tissue IL-10 levels. Muscle tissue collagen levels were not statistically different between the groups in parallel with our histological results. In our study, it was demonstrated that vitamin C has effects on inflammatory mediators during muscle tissue repair to explain the mechanism detailed molecular analyzes are needed.

 Musculoskeletal injuries as a kind of trauma that the human body is exposed to, adversely affect the quality of life and workforce of individuals due to restriction of movement function. This study aimed to evaluate the effects of dose-dependent ascorbic acid AA administration on the repair process after gastrocnemius muscle injury in rats. In this study, 5-month-old 66 male Wistar Albino rats were used and rats were randomly divided into 6 groups of 11 each control, muscle injury, healthy with 5 mg10 mgkgday AA-treated group, injury with 5 mg10 mgkgday AA-treated group. A linear incision was made in the gastrocnemius muscle of thirty-three animals included in the muscle injury groups. AA 5-10 mgkgday was administered to the four groups intraperitoneally just after surgery once a day. Animals were sacrificed twenty-one days later. Blood and tissue samples were used for cytokine, collagen, and histological measurements. It was found that a dose of 5 mgkgday AA administration reduced serum IL-6 and muscle tissue TNF-α levels, and increased liver tissue IL-10 levels. Muscle tissue collagen levels were not statistically different between the groups in parallel with our histological results. In our study, it was demonstrated that vitamin C has effects on inflammatory mediators during muscle tissue repair to explain the mechanism detailed molecular analyzes are needed.

 Musculoskeletal injuries as a kind of trauma that the human body is exposed to, adversely affect the quality of life and workforce of individuals due to restriction of movement function. This study aimed to evaluate the effects of dose-dependent ascorbic acid AA administration on the repair process after gastrocnemius muscle injury in rats. In this study, 5-month-old 66 male Wistar Albino rats were used and rats were randomly divided into 6 groups of 11 each control, muscle injury, healthy with 5 mg10 mgkgday AA-treated group, injury with 5 mg10 mgkgday AA-treated group. A linear incision was made in the gastrocnemius muscle of thirty-three animals included in the muscle injury groups. AA 5-10 mgkgday was administered to the four groups intraperitoneally just after surgery once a day. Animals were sacrificed twenty-one days later. Blood and tissue samples were used for cytokine, collagen, and histological measurements. It was found that a dose of 5 mgkgday AA administration reduced serum IL-6 and muscle tissue TNF-α levels, and increased liver tissue IL-10 levels. Muscle tissue collagen levels were not statistically different between the groups in parallel with our histological results. In our study, it was demonstrated that vitamin C has effects on inflammatory mediators during muscle tissue repair to explain the mechanism detailed molecular analyzes are needed.

 Musculoskeletal injuries as a kind of trauma that the human body is exposed to, adversely affect the quality of life and workforce of individuals due to restriction of movement function. This study aimed to evaluate the effects of dose-dependent ascorbic acid AA administration on the repair process after gastrocnemius muscle injury in rats. In this study, 5-month-old 66 male Wistar Albino rats were used and rats were randomly divided into 6 groups of 11 each control, muscle injury, healthy with 5 mg10 mgkgday AA-treated group, injury with 5 mg10 mgkgday AA-treated group. A linear incision was made in the gastrocnemius muscle of thirty-three animals included in the muscle injury groups. AA 5-10 mgkgday was administered to the four groups intraperitoneally just after surgery once a day. Animals were sacrificed twenty-one days later. Blood and tissue samples were used for cytokine, collagen, and histological measurements. It was found that a dose of 5 mgkgday AA administration reduced serum IL-6 and muscle tissue TNF-α levels, and increased liver tissue IL-10 levels. Muscle tissue collagen levels were not statistically different between the groups in parallel with our histological results. In our study, it was demonstrated that vitamin C has effects on inflammatory mediators during muscle tissue repair to explain the mechanism detailed molecular analyzes are needed.

Makale detayları

Effect of ascorbic acid on collagen and some inflammatory mediators in rats

Yazarlar:

Sibel Işın | Nuray Yazıhan | Burcu Kesikli | Derya Biriken | Sevginur Akdaş | Ferda Topal Celikkan | Batuhan Bakırarar | Pelin Arıbal-ayral

Yayıncı:

Ankara University

Ana Başlıklar:

Veterinary Medicine | Veterinary Surgery

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.70 Issue 3, pp.303-308

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001292292

pISSN:

1300-0861

ascorbic acid

collagen

inflammation mediators

muscle

wound healing

Sayı

Makale detayları

Effect of ascorbic acid on collagen and some inflammatory mediators in rats

Yazarlar: Sibel Işın | Nuray Yazıhan | Burcu Kesikli | Derya Biriken | Sevginur Akdaş | Ferda Topal Celikkan | Batuhan Bakırarar | Pelin Arıbal-ayral

Yayıncı: Ankara University

Ana Başlıklar: Veterinary Medicine | Veterinary Surgery

Dergi: Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.70 Issue 3, pp.303-308

Belge Tipi: Makale

Yayın Tarihi: 6/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001292292

pISSN: 1300-0861

ascorbic acid

collagen

inflammation mediators

muscle

wound healing

Sayı

Yazarlar:

Sibel Işın | Nuray Yazıhan | Burcu Kesikli | Derya Biriken | Sevginur Akdaş | Ferda Topal Celikkan | Batuhan Bakırarar | Pelin Arıbal-ayral

Yayıncı:

Ankara University

Ana Başlıklar:

Veterinary Medicine | Veterinary Surgery

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.70 Issue 3, pp.303-308

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001292292

pISSN:

1300-0861