GENETIC POLYMORPHISM OF APE1 ASP148GLU IS NOT ASSOCIATED WITH BLADDER CANCER RISK IN A TURKISH POPULATION

Taghı Ahmadı Rendı; Selçuk Erdem; Mehmet Enes Değirmenci; Öner Şanlı; Canan Kucukgergin; Semra Dogru Abbasoglu

Makale detayları

GENETIC POLYMORPHISM OF APE1 ASP148GLU IS NOT ASSOCIATED WITH BLADDER CANCER RISK IN A TURKISH POPULATION

Yazarlar:

Taghı Ahmadı Rendı | Selçuk Erdem | Mehmet Enes Değirmenci | Öner Şanlı | Canan Kucukgergin | Semra Dogru Abbasoglu

Yayıncı:

Istanbul University

Ana Başlıklar:

Clinical Sciences

Dergi:

Journal of Advanced Research in Health Sciences Vol.6 Issue 2, pp.117-121

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001302271

pISSN:

2651-4060

Anahtar kelimeler:

Özet:

 Objective: The purpose of this investigative research was to investigatethe potential impact of a single nucleotide polymorphism Asp148Glu within the APE1 gene on the development of bladder cancer BCa andspread, and to investigate the interaction of this polymorphism withcigarette smoking in BCa patients. Materials and Methods: In a study of 256 participants, consisting of 136 healthy individuals and 120 patients with BCa, the APE1 Asp148Glu polymorphism was evaluated using polymerase chain reaction restrictionfragment length polymorphism analysis. Results: There were no noteworthy difference variations observed in genotype distribution between the group of individuals with BCa and the control group with regards to the APE1 gene polymorphism. Furthermore, the APE1 gene polymorphism exhibited no correlation with the clinicopathological characteristics or smoking habits of individuals with BCa. Conclusion: Based on our findings, it appears that the APE1 gene polymorphism, which plays a role in the BER pathway, does not appear to be a contributing factor in susceptibility to BCa in the Turkish population. In addition, the smoking habit may not modify BCa risk with respect to genetic variations in the APE1 gene.

 Objective: The purpose of this investigative research was to investigatethe potential impact of a single nucleotide polymorphism Asp148Glu within the APE1 gene on the development of bladder cancer BCa andspread, and to investigate the interaction of this polymorphism withcigarette smoking in BCa patients. Materials and Methods: In a study of 256 participants, consisting of 136 healthy individuals and 120 patients with BCa, the APE1 Asp148Glu polymorphism was evaluated using polymerase chain reaction restrictionfragment length polymorphism analysis. Results: There were no noteworthy difference variations observed in genotype distribution between the group of individuals with BCa and the control group with regards to the APE1 gene polymorphism. Furthermore, the APE1 gene polymorphism exhibited no correlation with the clinicopathological characteristics or smoking habits of individuals with BCa. Conclusion: Based on our findings, it appears that the APE1 gene polymorphism, which plays a role in the BER pathway, does not appear to be a contributing factor in susceptibility to BCa in the Turkish population. In addition, the smoking habit may not modify BCa risk with respect to genetic variations in the APE1 gene.

 Objective: The purpose of this investigative research was to investigatethe potential impact of a single nucleotide polymorphism Asp148Glu within the APE1 gene on the development of bladder cancer BCa andspread, and to investigate the interaction of this polymorphism withcigarette smoking in BCa patients. Materials and Methods: In a study of 256 participants, consisting of 136 healthy individuals and 120 patients with BCa, the APE1 Asp148Glu polymorphism was evaluated using polymerase chain reaction restrictionfragment length polymorphism analysis. Results: There were no noteworthy difference variations observed in genotype distribution between the group of individuals with BCa and the control group with regards to the APE1 gene polymorphism. Furthermore, the APE1 gene polymorphism exhibited no correlation with the clinicopathological characteristics or smoking habits of individuals with BCa. Conclusion: Based on our findings, it appears that the APE1 gene polymorphism, which plays a role in the BER pathway, does not appear to be a contributing factor in susceptibility to BCa in the Turkish population. In addition, the smoking habit may not modify BCa risk with respect to genetic variations in the APE1 gene.

 Amaç: Çalışmamızda, APE1 Asp148Glu gen polimorfizmi ile mesane kanseri oluşumu ve gelişimi arasındaki ilişkiyi araştırmayı amaçladık. Ayrıca, mesane kanserinde sigara kullanımı ile APE 1 gen polimorfizmi arasındaki ilişkiyi değerlendirmeyi planladık. Gereç ve yöntem: Çalışmamızda histopatolojik ve klinik açıdan mesane kanseri tanısı konan hastalar n120 ve sağlıklı normal kişiler n136 yer aldı. Çalışma grubundaki kişilerden elde edilen DNAlardan Apürinik Aprimidinik Endonükleaz 1 APE1 gen polimorfizmi için polimeraz zincir reaksiyonu PZR, sınırlayıcı enzim parça uzunluğu polimorfizmi RFLP teknikleri kullanıldı. Bulgular: APE1 gen polimorfizminde genotip ve allel sıklığı incelendiğinde mesane kanseri ve kontrol grubu arasında anlamlı farklılık bulunmamaktadır. İlaveten, yüksek grade, ileri evre ve sigara kullanımı bakımından da incelendiğinde anlamlı bir faklılık bulunmamaktadır. Sonuç: APE 1 gen polimorfizminin mesane kanseri oluşumunda ve gelişiminde bir risk içermediğini ayrıca, sigara kullanımının APE Asp148Glu gen polimorfizminde etkili olmadığını ileri sürebiliriz.

Makale detayları

GENETIC POLYMORPHISM OF APE1 ASP148GLU IS NOT ASSOCIATED WITH BLADDER CANCER RISK IN A TURKISH POPULATION

Yazarlar:

Taghı Ahmadı Rendı | Selçuk Erdem | Mehmet Enes Değirmenci | Öner Şanlı | Canan Kucukgergin | Semra Dogru Abbasoglu

Yayıncı:

Istanbul University

Ana Başlıklar:

Clinical Sciences

Dergi:

Journal of Advanced Research in Health Sciences Vol.6 Issue 2, pp.117-121

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001302271

pISSN:

2651-4060

APE1

polymorphism

base excision repair

bladder cancerAPE1

polimorfizm

baz kesip çıkarma onarımı

mesane kanseri

Sayı

Makale detayları

Yazarlar: Taghı Ahmadı Rendı | Selçuk Erdem | Mehmet Enes Değirmenci | Öner Şanlı | Canan Kucukgergin | Semra Dogru Abbasoglu

Yayıncı: Istanbul University

Ana Başlıklar: Clinical Sciences

Dergi: Journal of Advanced Research in Health Sciences Vol.6 Issue 2, pp.117-121

Belge Tipi: Makale

Yayın Tarihi: 6/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001302271

pISSN: 2651-4060

Sayı

Yazarlar:

Taghı Ahmadı Rendı | Selçuk Erdem | Mehmet Enes Değirmenci | Öner Şanlı | Canan Kucukgergin | Semra Dogru Abbasoglu

Yayıncı:

Istanbul University

Ana Başlıklar:

Clinical Sciences

Dergi:

Journal of Advanced Research in Health Sciences Vol.6 Issue 2, pp.117-121

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001302271

pISSN:

2651-4060