Serum Protein Profile of Juvenile Pacu (Piaractus mesopotamicus) and Dourado (Salminus brasiliensis) Fed Bovine Colostrum as Partial Source of Protein in the Diet

Debora Moretti; Wiolene Nordi; Raul Machado-Neto

Makale detayları

Serum Protein Profile of Juvenile Pacu (Piaractus mesopotamicus) and Dourado (Salminus brasiliensis) Fed Bovine Colostrum as Partial Source of Protein in the Diet

Yazarlar:

Debora Moretti | Wiolene Nordi | Raul Machado-Neto

Yayıncı:

Istanbul University

Dergi:

European Journal of Biology 2018 Vol.77 Issue 2, pp.59-64

Belge Tipi:

Makale

Yayın Tarihi:

12/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000424458

eISSN:

2618-6144

pISSN:

2602-2575

Özet:

 


DOI: 10.26650/EurJBiol.2018.400108


Objective: The electrophoretic profile of
serum proteins was investigated in juvenile Piaractus mesopotamicus and
Salminus brasiliensis fed with diets containing bovine colostrum, a
nutraceutical food, in lyophilized form (LBC) for either 30 or 60 days.


Materials and Methods: Blood samples were
collected from juveniles of P. mesopotamicus and S. brasiliensis fed for either
30 or 60 days with diets containing 0%, 10% or 20% of LBC. Serum protein
fractions were then determined by electrophoresis in agarose gel.


Results: The P. mesopotamicus, an
omnivorous fish, showed six serum protein fractions, while the S. brasiliensis,
a carnivorous fish, showed four serum protein fractions. In both species the
albumin fraction showed higher protein content at 30 days than at 60 days (p<0.05),
indicating an inverse relationship with growth. The 5th and 4th fraction in
mobility from the P. mesopotamicus and S. brasiliensis serum, respectively,
were positioned in a gamma-globulin zone. In P. mesopotamicus, the
concentration of protein in the gamma-globulin zone was higher at 60 days than
at 30 days (p<0.05). In S. brasiliensis, in turn, the concentration of
protein in the gamma-globulin zone was higher at 30 days than at 60 days
(p<0.05). In juvenile P. mesopotamicus, a higher concentration of protein
was also observed in the gamma-globulin zone in the 0% LBC compared to 10% and
20% LBC (p<0.05). Thus, the feeding period influenced only the fractions
that were positioned in the albumin and gamma-globulin migration zone in both
species. 









Conclusion: The consumption of bovine
colostrum decreased the concentration of protein in gamma-globulin fraction of
the P. mesopotamicus, indicating that these juveniles had either less stimulus
for their own synthesis of defense elements or an immunosuppressive effect of
bovine colostrum ingestion.


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Makale detayları

Serum Protein Profile of Juvenile Pacu (Piaractus mesopotamicus) and Dourado (Salminus brasiliensis) Fed Bovine Colostrum as Partial Source of Protein in the Diet

Yazarlar: Debora Moretti | Wiolene Nordi | Raul Machado-Neto

Yayıncı: Istanbul University

Dergi: European Journal of Biology 2018 Vol.77 Issue 2, pp.59-64

Belge Tipi: Makale

Yayın Tarihi: 12/2018

Belge Dili: İngilizce

Document ID (Org No.): 2000424458

eISSN: 2618-6144

pISSN: 2602-2575

Sayı

Yazarlar:

Debora Moretti | Wiolene Nordi | Raul Machado-Neto

Yayıncı:

Istanbul University

Dergi:

European Journal of Biology 2018 Vol.77 Issue 2, pp.59-64

Belge Tipi:

Makale

Yayın Tarihi:

12/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000424458

eISSN:

2618-6144

pISSN:

2602-2575