Distributions and Possible Roles of Laminin, Fibronectin and Their  Receptor Subunits Integrin b4 and a5 in Remodelling of Extracellular  Matrix during Decidualization in Rats

Ümit Ali KAYIŞLI; M. AŞAR; R. DEMİR

Makale detayları

Distributions and Possible Roles of Laminin, Fibronectin and Their Receptor Subunits Integrin b4 and a5 in Remodelling of Extracellular Matrix during Decidualization in Rats

Yazarlar:

Ümit Ali KAYIŞLI | M. AŞAR | R. DEMİR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2000 Vol.24 Issue 3, pp.379-396

Belge Tipi:

Makale

Yayın Tarihi:

2/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000813969

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

In the endometrium preparing for pregnancy, the stromal matrix components of the cells  and their products begin structural differentiation due to endometrial receptivity. According to the  hypothesis that 'extracellular matrix (ECM) proteins and adhesion molecules take roles in the structural  differentiation as a result of endometrial decidualization', our aim was to determine the roles of ECM  molecules laminin, fibronectin and adhesion proteins integrin a5 and b4 in the decidulization and implantation by  examining the emergence and changing distribution of these proteins.  In this study, five experimental groups, consisting of 40 pregnant animals on pregnancy days 4-8, were used. Paraffin  tissue serial sections that were 6-8 µm in thickness from the implantation areas were stained by immunohistochemical  techniques.  Decidual reaction area (DRA) was first observed on pregnancy day 4. Fibronectin was observed densely in the center of  the DRA on pregnancy days 4, 5 and in the primary decidual area (PDRA) on pregnancy days 6-8. Although integrin a5  had a similar staining to fibronectin up to pregnancy day 6, it was very weak in PDRA after that day. While laminin was  dense in the basal lamina of the lumen and glandular epithelium and in the periphery of the DRA at the beginning, it  was observed to be dense in the secondary decidual area (SDRA) after pregnancy day 6 and to have paralel  immunoreactivity to integrin b4 on all pregnancy days.  In conclusion, we can say that decidual reaction appears on pregnancy days 4-8 and not only laminin and fibronectin but  also integrin a5 and b4 take part actively in decidual tissue formation and implantation events in the endometrium of  rat uterus.

 

In the endometrium preparing for pregnancy, the stromal matrix components of the cells  and their products begin structural differentiation due to endometrial receptivity. According to the  hypothesis that 'extracellular matrix (ECM) proteins and adhesion molecules take roles in the structural  differentiation as a result of endometrial decidualization', our aim was to determine the roles of ECM  molecules laminin, fibronectin and adhesion proteins integrin a5 and b4 in the decidulization and implantation by  examining the emergence and changing distribution of these proteins.  In this study, five experimental groups, consisting of 40 pregnant animals on pregnancy days 4-8, were used. Paraffin  tissue serial sections that were 6-8 µm in thickness from the implantation areas were stained by immunohistochemical  techniques.  Decidual reaction area (DRA) was first observed on pregnancy day 4. Fibronectin was observed densely in the center of  the DRA on pregnancy days 4, 5 and in the primary decidual area (PDRA) on pregnancy days 6-8. Although integrin a5  had a similar staining to fibronectin up to pregnancy day 6, it was very weak in PDRA after that day. While laminin was  dense in the basal lamina of the lumen and glandular epithelium and in the periphery of the DRA at the beginning, it  was observed to be dense in the secondary decidual area (SDRA) after pregnancy day 6 and to have paralel  immunoreactivity to integrin b4 on all pregnancy days.  In conclusion, we can say that decidual reaction appears on pregnancy days 4-8 and not only laminin and fibronectin but  also integrin a5 and b4 take part actively in decidual tissue formation and implantation events in the endometrium of  rat uterus.

Makale detayları

Distributions and Possible Roles of Laminin, Fibronectin and Their Receptor Subunits Integrin b4 and a5 in Remodelling of Extracellular Matrix during Decidualization in Rats

Yazarlar:

Ümit Ali KAYIŞLI | M. AŞAR | R. DEMİR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2000 Vol.24 Issue 3, pp.379-396

Belge Tipi:

Makale

Yayın Tarihi:

2/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000813969

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Laminin

fibronectin

integrin

decidualization

implantation.

Sayı

Makale detayları

Distributions and Possible Roles of Laminin, Fibronectin and Their Receptor Subunits Integrin b4 and a5 in Remodelling of Extracellular Matrix during Decidualization in Rats

Yazarlar: Ümit Ali KAYIŞLI | M. AŞAR | R. DEMİR

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2000 Vol.24 Issue 3, pp.379-396

Belge Tipi: Makale

Yayın Tarihi: 2/2000

Belge Dili: Türkçe

Document ID (Org No.): 2000813969

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Laminin

fibronectin

integrin

decidualization

implantation.

Sayı

Yazarlar:

Ümit Ali KAYIŞLI | M. AŞAR | R. DEMİR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2000 Vol.24 Issue 3, pp.379-396

Belge Tipi:

Makale

Yayın Tarihi:

2/2000

Belge Dili:

Türkçe

Document ID (Org No.):

2000813969

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152