Impact of Cryopreservation on Caspase-3 Gene Expression, Cell Viability, and Sperm Motility in Bull Testicular Tissue and Epididymal Sperm

Cumali Kaya; Burcu Esin; Cansu Can; Eda Turgut Uğurtay; Mesut Çevik

Makale detayları

Impact of Cryopreservation on Caspase-3 Gene Expression, Cell Viability, and Sperm Motility in Bull Testicular Tissue and Epididymal Sperm

Yazarlar:

Cumali Kaya | Burcu Esin | Cansu Can | Eda Turgut Uğurtay | Mesut Çevik

Yayıncı:

Burdur Mehmet Akif Ersoy University

Ana Başlıklar:

Reproduction and Artificial Insemination

Dergi:

Mediterranean Veterinary Journal Vol.10 Issue 2, pp.496-503

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357650

pISSN:

3023-8447

Anahtar kelimeler:

Özet:

 This study investigated the effects of cryopreservation of testicular tissue and epididymal sperm in bulls on spermatological parameters, cell viability, and caspase-3 gene expression, a marker of apoptosis. Epididymal sperm and testicular tissue pieces obtained from testes collected from bulls n: 15 were cryopreserved and stored in liquid nitrogen -196C. Epididymal sperm were frozen in liquid nitrogen vapor using the straw method, while testicular tissue pieces were frozen in cryotubes using a slow freezing protocol with Dimethylsulfoxide DMSO and Ethylene Glycol EG as cryoprotectants. Fresh semen exhibited significantly higher motility, progressive motility, kinematic parameters, and viability values compared to those after thawing P  0.05. Higher cell viability was achieved in DMSO-cryopreserved testicular tissues 53.72  6.36 compared to EG-cryopreserved testicular tissues 41.83  7.22 P  0.05. Caspase-3 gene expression was determined to be 42.66  98.26-fold increased in DMSO-cryopreserved tissues and 65.05  106.03 fold increased in EG-cryopreserved tissues after thawing. In epididymal sperm, gene expression levels increased by an average of 12.22  34.60 fold compared to fresh samples. These findings suggest that cryopreservation affects cellular integrity and triggers apoptotic pathways and repair mechanisms, highlighting the need to optimize protocols to reduce cryo-induced damage and increase post-thaw viability.

 This study investigated the effects of cryopreservation of testicular tissue and epididymal sperm in bulls on spermatological parameters, cell viability, and caspase-3 gene expression, a marker of apoptosis. Epididymal sperm and testicular tissue pieces obtained from testes collected from bulls n: 15 were cryopreserved and stored in liquid nitrogen -196C. Epididymal sperm were frozen in liquid nitrogen vapor using the straw method, while testicular tissue pieces were frozen in cryotubes using a slow freezing protocol with Dimethylsulfoxide DMSO and Ethylene Glycol EG as cryoprotectants. Fresh semen exhibited significantly higher motility, progressive motility, kinematic parameters, and viability values compared to those after thawing P  0.05. Higher cell viability was achieved in DMSO-cryopreserved testicular tissues 53.72  6.36 compared to EG-cryopreserved testicular tissues 41.83  7.22 P  0.05. Caspase-3 gene expression was determined to be 42.66  98.26-fold increased in DMSO-cryopreserved tissues and 65.05  106.03 fold increased in EG-cryopreserved tissues after thawing. In epididymal sperm, gene expression levels increased by an average of 12.22  34.60 fold compared to fresh samples. These findings suggest that cryopreservation affects cellular integrity and triggers apoptotic pathways and repair mechanisms, highlighting the need to optimize protocols to reduce cryo-induced damage and increase post-thaw viability.

 This study investigated the effects of cryopreservation of testicular tissue and epididymal sperm in bulls on spermatological parameters, cell viability, and caspase-3 gene expression, a marker of apoptosis. Epididymal sperm and testicular tissue pieces obtained from testes collected from bulls n: 15 were cryopreserved and stored in liquid nitrogen -196C. Epididymal sperm were frozen in liquid nitrogen vapor using the straw method, while testicular tissue pieces were frozen in cryotubes using a slow freezing protocol with Dimethylsulfoxide DMSO and Ethylene Glycol EG as cryoprotectants. Fresh semen exhibited significantly higher motility, progressive motility, kinematic parameters, and viability values compared to those after thawing P  0.05. Higher cell viability was achieved in DMSO-cryopreserved testicular tissues 53.72  6.36 compared to EG-cryopreserved testicular tissues 41.83  7.22 P  0.05. Caspase-3 gene expression was determined to be 42.66  98.26-fold increased in DMSO-cryopreserved tissues and 65.05  106.03 fold increased in EG-cryopreserved tissues after thawing. In epididymal sperm, gene expression levels increased by an average of 12.22  34.60 fold compared to fresh samples. These findings suggest that cryopreservation affects cellular integrity and triggers apoptotic pathways and repair mechanisms, highlighting the need to optimize protocols to reduce cryo-induced damage and increase post-thaw viability.

 Bu çalışmada, boğalarda testis dokusu ve epididimal sperma kriyoprezervasyonunun spermatolojik parametreler, hücre canlılığı ve apoptozis belirteci olan kaspaz-3 gen ekspresyonu üzerine etkileri araştırılmıştır. Boğalardan toplanan testislerden elde edilen epididimal sperma ve testis dokusu parçaları n: 15 kriyoprezervasyon sonrasında sıvı nitrojende -196 C saklanmıştır. Epididimal sperma payet yöntemi kullanılarak sıvı nitrojen buharında dondurulurken, testis dokusu parçaları kriyoprotektan olarak Dimetilsülfoksit DMSO ve Etilen Glikol EG içeren kriyotüplerde yavaş dondurma protokolü kullanılarak dondurulmuştur. Taze sperma, çözüm sonrası örneklere kıyasla önemli ölçüde daha yüksek motilite, progresif motilite, kinematik parametreler ve canlılık değerleri göstermiştir P  0,05. DMSO ile kriyoprezerve edilen testis dokularında 53.72  6.36, EG ile kriyoprezerve edilen testis dokularına 41.83  7.22 kıyasla daha yüksek hücre canlılığı elde edildi P  0.05. Kaspaz-3 gen ekspresyonunun çözüm sonrası DMSO grubundaki dokularda 42.66  98.26 kat, EG grubundaki dokularda ise 65.05  106.03 kat arttığı belirlendi. Epididimal spermade gen ekspresyon düzeyleri taze spermaya kıyasla çözüm sonrası spermada ortalama 12.22  34.60 kat arttı. Bu bulgular kriyoprezervasyonun hücre bütünlüğünü etkilediğini ve apoptotik yolları ve onarım mekanizmalarını tetiklediğini düşündürerek, kriyo hasarı azaltmak ve çözüm sonrası canlılığı artırmak için protokolleri optimize etme ihtiyacını vurgulamaktadır.

Makale detayları

Impact of Cryopreservation on Caspase-3 Gene Expression, Cell Viability, and Sperm Motility in Bull Testicular Tissue and Epididymal Sperm

Yazarlar:

Cumali Kaya | Burcu Esin | Cansu Can | Eda Turgut Uğurtay | Mesut Çevik

Yayıncı:

Burdur Mehmet Akif Ersoy University

Ana Başlıklar:

Reproduction and Artificial Insemination

Dergi:

Mediterranean Veterinary Journal Vol.10 Issue 2, pp.496-503

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357650

pISSN:

3023-8447

cryopreservation

caspase-3

epididiymal sperm

gene expression

testicular tissuekriyoprezervasyon

kaspaz-3

epididimal sperma

gen ekspresyonu

testis dokusu

Sayı

Makale detayları

Yazarlar: Cumali Kaya | Burcu Esin | Cansu Can | Eda Turgut Uğurtay | Mesut Çevik

Yayıncı: Burdur Mehmet Akif Ersoy University

Ana Başlıklar: Reproduction and Artificial Insemination

Dergi: Mediterranean Veterinary Journal Vol.10 Issue 2, pp.496-503

Belge Tipi: Makale

Yayın Tarihi: 8/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001357650

pISSN: 3023-8447

Sayı

Yazarlar:

Cumali Kaya | Burcu Esin | Cansu Can | Eda Turgut Uğurtay | Mesut Çevik

Yayıncı:

Burdur Mehmet Akif Ersoy University

Ana Başlıklar:

Reproduction and Artificial Insemination

Dergi:

Mediterranean Veterinary Journal Vol.10 Issue 2, pp.496-503

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357650

pISSN:

3023-8447