The Effects of Quercetin and Hyperthermia on OVCAR-3 Cells

Özlem ÖZGÜR GÜRSOY; Canan VEJSELOVA SEZER; Ceren YILDIZ EREN; Hulusi Göktuğ GÜRER

doi:https://doi.org/10.47493/abantmedj.881050

Makale detayları

The Effects of Quercetin and Hyperthermia on OVCAR-3 Cells

Yazarlar:

Özlem ÖZGÜR GÜRSOY | Canan VEJSELOVA SEZER | Ceren YILDIZ EREN | Hulusi Göktuğ GÜRER

Yayıncı:

Bolu Abant Izzet Baysal University

Ana Başlıklar:

Hastalıklar

Dergi:

Abant Medical Journal 2021 Vol.10 Issue 2, pp.212-218

Belge Tipi:

Makale

Yayın Tarihi:

8/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000435643

Sıklık:

Üç Yıllık

eISSN:

2147-1800

pISSN:

1305-4392

DOI:

https://doi.org/10.47493/abantmedj.881050

Özet:

 


INTRODUCTION: This study aims to exhibit the growth inhibitory effect of quercetin on the human ovarian epithelial carcinoma cells (OVCAR-3) and scrutinize the effect of hyperthermia.METHODS: OVCAR-3 cells were exposed to different concentrations of quercetin and apoptotic changes were recorded with confocal microscopy and transmission electron microscopy. Also Annexin-V and reactive oxygen species (ROS) measurement were analyzed. Cytotoxicity was tested on quercetin exposed OVCAR-3 cells with hyperthermia.RESULTS: Quercetin has good anticancerous activities in vitro by inhibiting the proliferation of OVCAR-3 cells. The recorded IC50 value of quercetin was 122 μM for 48 hours. Hyperthermia didnot influence significantly on the growth inhibition of OVCAR-3 cells applied with quercetin.DISCUSSION and CONCLUSION: Our results demonstrated that quercetin could induce apoptosis in a time and concentration-dependent manner in OVCAR-3 cells.

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Aim: the aim of this study is to exhibit the growth inhibitory effect  of quercetin on the human ovarian epithelial carcinoma cells (OVCAR-3) and scrutinize the effect of  hyperthermia.
Materials and Methods: OVCAR-3 cells were exposed to different concentrations of quercetin and apoptotic changes were recorded with confocal microscopy and transmission electron microscopy. Also Annexin-V analysis and ROS measurement were analyzed. Cytotoxicity was tested on quercetin exposed OVCAR-3 cells with hyperthermia.
Results: Quercetin have good anticancerous activities in vitro by inhibiting the proliferation of OVCAR-3 cells. The recorded IC50 value of quercetin was 122 μM for 48 hours. Hyperthermia didnot influence significantly on the growth inhibition of OVCAR-3 cells applied with quercetin.
Conclusion: Our results demonstrated that quercetin could induce apoptosis in a time and concentration-dependent manner in OVCAR-3 cells.

Makale detayları

The Effects of Quercetin and Hyperthermia on OVCAR-3 Cells

Yazarlar: Özlem ÖZGÜR GÜRSOY | Canan VEJSELOVA SEZER | Ceren YILDIZ EREN | Hulusi Göktuğ GÜRER

Yayıncı: Bolu Abant Izzet Baysal University

Ana Başlıklar: Hastalıklar

Dergi: Abant Medical Journal 2021 Vol.10 Issue 2, pp.212-218

Belge Tipi: Makale

Yayın Tarihi: 8/2021

Belge Dili: İngilizce

Document ID (Org No.): 2000435643

Sıklık: Üç Yıllık

eISSN: 2147-1800

pISSN: 1305-4392

DOI: https://doi.org/10.47493/abantmedj.881050

Sayı

Yazarlar:

Özlem ÖZGÜR GÜRSOY | Canan VEJSELOVA SEZER | Ceren YILDIZ EREN | Hulusi Göktuğ GÜRER

Yayıncı:

Bolu Abant Izzet Baysal University

Ana Başlıklar:

Hastalıklar

Dergi:

Abant Medical Journal 2021 Vol.10 Issue 2, pp.212-218

Belge Tipi:

Makale

Yayın Tarihi:

8/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000435643

Sıklık:

Üç Yıllık

eISSN:

2147-1800

pISSN:

1305-4392

DOI:

https://doi.org/10.47493/abantmedj.881050