Analysis of WRKY Transcription Factors in Barley Cultivars Infected with Fusarium culmorum

Ebru ULUHAN; Esra Nur KELEŞ; Feyza TUFAN

doi:https://doi.org/10.38001/ijlsb.588730

Makale detayları

Analysis of WRKY Transcription Factors in Barley Cultivars Infected with Fusarium culmorum

Yazarlar:

Ebru ULUHAN | Esra Nur KELEŞ | Feyza TUFAN

Yayıncı:

International Society of Academicians

Ana Başlıklar:

Genetik ve evrim

Dergi:

International Journal of Life Sciences and Biotechnology 2019 Vol.2 Issue 3, pp.165-174

Belge Tipi:

Makale

Yayın Tarihi:

12/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000467154

Sıklık:

Üç Yıllık

eISSN:

2651-4621

DOI:

https://doi.org/10.38001/ijlsb.588730

Özet:

 

One of the most critical problems of cereal breeding
is Fusarium crown rot disease caused
by various Fusarium species. Fusarium culmorum is one of the
predominant pathogen in Turkey and causes serious product losses. In this
study, the early response of barley cultivars upon F. culmorum infection were analyzed by disease severity and gene
expression patterns of WRKY transcription factors. In that context, firstly,
disease severities of 9 barley cultivars (Hordeum
vulgare L. cvs. Epona, Escadre, Gazda, Oliver, Avcı 2002, Burakbey, Tarm
92, Manava, and Ramata) infected with F.
culmorum were determined with disease index percentages. After 7 days of
infection, Epona was more sensitive than the other cultivars while the lowest
disease index was seen in Gazda. Total RNA extractions were performed at 72 hai
from the root tissues of Epona and Gazda. Expression analysis of HvWRKY6, HvWRKY9, HvWRKY24, HvWRKY25, HvWRKY33, HvWRKY34, HvWRKY42, and HvWRKY46 genes were
conducted by qPCR. As a result of pathogen stress, it was observed that the
transcript levels of HvWRKY33 was
significantly upregulated in both cultivars. HvWRKY6, HvWRKY34 and HvWRKY46 genes were increased in Epona
while upregulation of HvWRKY25 and HvWRKY34 genes were detected in Gazda.
No significant decreases were detected in any cultivars. This study is
important in terms of providing an association between WRKY genes and pathogen stress response.

Makale detayları

Analysis of WRKY Transcription Factors in Barley Cultivars Infected with Fusarium culmorum

Yazarlar: Ebru ULUHAN | Esra Nur KELEŞ | Feyza TUFAN

Yayıncı: International Society of Academicians

Ana Başlıklar: Genetik ve evrim

Dergi: International Journal of Life Sciences and Biotechnology 2019 Vol.2 Issue 3, pp.165-174

Belge Tipi: Makale

Yayın Tarihi: 12/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000467154

Sıklık: Üç Yıllık

eISSN: 2651-4621

DOI: https://doi.org/10.38001/ijlsb.588730

Sayı

Yazarlar:

Ebru ULUHAN | Esra Nur KELEŞ | Feyza TUFAN

Yayıncı:

International Society of Academicians

Ana Başlıklar:

Genetik ve evrim

Dergi:

International Journal of Life Sciences and Biotechnology 2019 Vol.2 Issue 3, pp.165-174

Belge Tipi:

Makale

Yayın Tarihi:

12/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000467154

Sıklık:

Üç Yıllık

eISSN:

2651-4621

DOI:

https://doi.org/10.38001/ijlsb.588730