Effects of Grayanotoxin-III on different cell lines: in vitro ischemia model

Esin Akbay Çetin; Çiğdem Özenirler

Makale detayları

Effects of Grayanotoxin-III on different cell lines: in vitro ischemia model

Yazarlar:

Esin Akbay Çetin | Çiğdem Özenirler

Yayıncı:

İzzet KARA

Ana Başlıklar:

Structural Biology | Plant Biochemistry

Dergi:

International Journal of Secondary Metabolite Vol.10 Issue 3, pp.370-384

Belge Tipi:

Makale

Yayın Tarihi:

8/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293612

pISSN:

2148-6905

Anahtar kelimeler:

Özet:

 Grayanotoxins GTXs are natural products and are mostly found in plants of the Ericaceae family, especially in the Rhododendron. With their ability to bind to voltage-gated sodium channels, they keep these channels constantly active and cause tissue damage. However, despite this feature, the use of Rhododendron leaves or its secondary products as an alternative product is especially common in Turkey. This study aims to evaluate the possible dose-related effects of GTX-III in ischemia-induced in vitro cell models. Within the scope of the study, an ischemia model was established in two different cell lines H9c2 and Cos-7 and treated with various concentrations of GTX-III. In this context, cell viability, cytotoxicity, apoptosis and necrosis were examined. In the results of MTT, a significant decrease p  0.05 in cell viability was observed in all GTX-III concentrations in H9c2 cells compared to the control, while a significant difference p  0.05 was observed in Cos-7 cells, especially at the 24th hour. LDH cytotoxicity was increased in a dose-dependent manner in both cell models. It was concluded that GTX-III caused apoptosis, and reduced cell viability in ischemia models; however, promoted cell proliferation in healthy cells. Based on the literature review, this study is the first to document the cytotoxic properties and apoptotic potential of GTX-III in an in vitro cell culture ischemia model. Our findings support the usage of GTX-III, however it should be remembered that the dose needs to be verified before being used medically.

 Grayanotoxins GTXs are natural products and are mostly found in plants of the Ericaceae family, especially in the Rhododendron. With their ability to bind to voltage-gated sodium channels, they keep these channels constantly active and cause tissue damage. However, despite this feature, the use of Rhododendron leaves or its secondary products as an alternative product is especially common in Turkey. This study aims to evaluate the possible dose-related effects of GTX-III in ischemia-induced in vitro cell models. Within the scope of the study, an ischemia model was established in two different cell lines H9c2 and Cos-7 and treated with various concentrations of GTX-III. In this context, cell viability, cytotoxicity, apoptosis and necrosis were examined. In the results of MTT, a significant decrease p  0.05 in cell viability was observed in all GTX-III concentrations in H9c2 cells compared to the control, while a significant difference p  0.05 was observed in Cos-7 cells, especially at the 24th hour. LDH cytotoxicity was increased in a dose-dependent manner in both cell models. It was concluded that GTX-III caused apoptosis, and reduced cell viability in ischemia models; however, promoted cell proliferation in healthy cells. Based on the literature review, this study is the first to document the cytotoxic properties and apoptotic potential of GTX-III in an in vitro cell culture ischemia model. Our findings support the usage of GTX-III, however it should be remembered that the dose needs to be verified before being used medically.

 Grayanotoxins GTXs are natural products and are mostly found in plants of the Ericaceae family, especially in the Rhododendron. With their ability to bind to voltage-gated sodium channels, they keep these channels constantly active and cause tissue damage. However, despite this feature, the use of Rhododendron leaves or its secondary products as an alternative product is especially common in Turkey. This study aims to evaluate the possible dose-related effects of GTX-III in ischemia-induced in vitro cell models. Within the scope of the study, an ischemia model was established in two different cell lines H9c2 and Cos-7 and treated with various concentrations of GTX-III. In this context, cell viability, cytotoxicity, apoptosis and necrosis were examined. In the results of MTT, a significant decrease p  0.05 in cell viability was observed in all GTX-III concentrations in H9c2 cells compared to the control, while a significant difference p  0.05 was observed in Cos-7 cells, especially at the 24th hour. LDH cytotoxicity was increased in a dose-dependent manner in both cell models. It was concluded that GTX-III caused apoptosis, and reduced cell viability in ischemia models; however, promoted cell proliferation in healthy cells. Based on the literature review, this study is the first to document the cytotoxic properties and apoptotic potential of GTX-III in an in vitro cell culture ischemia model. Our findings support the usage of GTX-III, however it should be remembered that the dose needs to be verified before being used medically.

 Grayanotoxins GTXs are natural products and are mostly found in plants of the Ericaceae family, especially in the Rhododendron. With their ability to bind to voltage-gated sodium channels, they keep these channels constantly active and cause tissue damage. However, despite this feature, the use of Rhododendron leaves or its secondary products as an alternative product is especially common in Turkey. This study aims to evaluate the possible dose-related effects of GTX-III in ischemia-induced in vitro cell models. Within the scope of the study, an ischemia model was established in two different cell lines H9c2 and Cos-7 and treated with various concentrations of GTX-III. In this context, cell viability, cytotoxicity, apoptosis and necrosis were examined. In the results of MTT, a significant decrease p  0.05 in cell viability was observed in all GTX-III concentrations in H9c2 cells compared to the control, while a significant difference p  0.05 was observed in Cos-7 cells, especially at the 24th hour. LDH cytotoxicity was increased in a dose-dependent manner in both cell models. It was concluded that GTX-III caused apoptosis, and reduced cell viability in ischemia models; however, promoted cell proliferation in healthy cells. Based on the literature review, this study is the first to document the cytotoxic properties and apoptotic potential of GTX-III in an in vitro cell culture ischemia model. Our findings support the usage of GTX-III, however it should be remembered that the dose needs to be verified before being used medically.

Makale detayları

Effects of Grayanotoxin-III on different cell lines: in vitro ischemia model

Yazarlar:

Esin Akbay Çetin | Çiğdem Özenirler

Yayıncı:

İzzet KARA

Ana Başlıklar:

Structural Biology | Plant Biochemistry

Dergi:

International Journal of Secondary Metabolite Vol.10 Issue 3, pp.370-384

Belge Tipi:

Makale

Yayın Tarihi:

8/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293612

pISSN:

2148-6905

Cardiomyocyte

Cells culture

Grayanotoxin-III

Renal cell line

CytotoxicityCardiomyocyte

Cells culture

Grayanotoxin-III

Renal cell line

Cytotoxicity

Sayı

Makale detayları

Yazarlar: Esin Akbay Çetin | Çiğdem Özenirler

Yayıncı: İzzet KARA

Ana Başlıklar: Structural Biology | Plant Biochemistry

Dergi: International Journal of Secondary Metabolite Vol.10 Issue 3, pp.370-384

Belge Tipi: Makale

Yayın Tarihi: 8/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001293612

pISSN: 2148-6905

Sayı

Yazarlar:

Esin Akbay Çetin | Çiğdem Özenirler

Yayıncı:

İzzet KARA

Ana Başlıklar:

Structural Biology | Plant Biochemistry

Dergi:

International Journal of Secondary Metabolite Vol.10 Issue 3, pp.370-384

Belge Tipi:

Makale

Yayın Tarihi:

8/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001293612

pISSN:

2148-6905