The Effects of the Pho85 Signaling Pathway on the Invertase Biosynthesis and Glucose Uptake in Saccharomyces cerevisiae

Sezai TÜRKEL*; Elif ARIK

Makale detayları

The Effects of the Pho85 Signaling Pathway on the Invertase Biosynthesis and Glucose Uptake in Saccharomyces cerevisiae

Yazarlar:

Sezai TÜRKEL* | Elif ARIK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2010 Vol.34 Issue 2, pp.103-108

Belge Tipi:

Makale

Yayın Tarihi:

1/2010

Belge Dili:

Türkçe

Document ID (Org No.):

2000813063

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Glucose signaling controls a wide range of metabolic events in the yeast Saccharomyces cerevisiae. Glucose sensing and signaling processes require a variety of membrane-bound and cytoplasmic sensor proteins. Pho85p is a cyclin-dependent protein kinase that controls different metabolic events upon binding to its cyclin partners in the cytoplasm of yeast cells. In this study, we investigated the roles of Pho85p in the glucose signaling pathways that control invertase biosynthesis and glucose uptake in yeast. The biosynthesis of invertase enzyme from the SUC2 gene is controlled by glucose repression and derepression mechanisms in S. cerevisiae. However, the results of this research indicated that invertase biosynthesis occurs at low levels in a deregulated manner in the Dpho85 mutant yeast strain. Furthermore, our results showed that the biosynthesis of invertase is not repressed when the Dpho85 mutant is exposed to high levels of glucose. Moreover, we found that the glucose consumption rate of the Dpho85 mutant is at least 2-fold lower than that of the wild-type yeast strain. Our results indicated that Pho85p functions are essential for the regulated biosynthesis of the invertase enzyme from the SUC2 gene and for the high levels of glucose uptake in S. cerevisiae.

 

Glucose signaling controls a wide range of metabolic events in the yeast Saccharomyces cerevisiae. Glucose sensing and signaling processes require a variety of membrane-bound and cytoplasmic sensor proteins. Pho85p is a cyclin-dependent protein kinase that controls different metabolic events upon binding to its cyclin partners in the cytoplasm of yeast cells. In this study, we investigated the roles of Pho85p in the glucose signaling pathways that control invertase biosynthesis and glucose uptake in yeast. The biosynthesis of invertase enzyme from the SUC2 gene is controlled by glucose repression and derepression mechanisms in S. cerevisiae. However, the results of this research indicated that invertase biosynthesis occurs at low levels in a deregulated manner in the Dpho85 mutant yeast strain. Furthermore, our results showed that the biosynthesis of invertase is not repressed when the Dpho85 mutant is exposed to high levels of glucose. Moreover, we found that the glucose consumption rate of the Dpho85 mutant is at least 2-fold lower than that of the wild-type yeast strain. Our results indicated that Pho85p functions are essential for the regulated biosynthesis of the invertase enzyme from the SUC2 gene and for the high levels of glucose uptake in S. cerevisiae.

Makale detayları

The Effects of the Pho85 Signaling Pathway on the Invertase Biosynthesis and Glucose Uptake in Saccharomyces cerevisiae

Yazarlar:

Sezai TÜRKEL* | Elif ARIK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2010 Vol.34 Issue 2, pp.103-108

Belge Tipi:

Makale

Yayın Tarihi:

1/2010

Belge Dili:

Türkçe

Document ID (Org No.):

2000813063

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Key words: Pho85

glucose sensing

glucose uptake

Saccharomyces cerevisiae

invertase

Sayı

Makale detayları

The Effects of the Pho85 Signaling Pathway on the Invertase Biosynthesis and Glucose Uptake in Saccharomyces cerevisiae

Yazarlar: Sezai TÜRKEL* | Elif ARIK

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2010 Vol.34 Issue 2, pp.103-108

Belge Tipi: Makale

Yayın Tarihi: 1/2010

Belge Dili: Türkçe

Document ID (Org No.): 2000813063

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Key words: Pho85

glucose sensing

glucose uptake

Saccharomyces cerevisiae

invertase

Sayı

Yazarlar:

Sezai TÜRKEL* | Elif ARIK

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2010 Vol.34 Issue 2, pp.103-108

Belge Tipi:

Makale

Yayın Tarihi:

1/2010

Belge Dili:

Türkçe

Document ID (Org No.):

2000813063

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152