Effect of Zuclopenthixol Acetate on Neural Tube Development in Early Chick Embryos

Gülan Albaş Kurt; Alperen Sarıtaş; Emre Atay; Ayşe Ertekin; Yunus Emre Kundakcı; Tolga Ertekin

Makale detayları

Effect of Zuclopenthixol Acetate on Neural Tube Development in Early Chick Embryos

Yazarlar:

Gülan Albaş Kurt | Alperen Sarıtaş | Emre Atay | Ayşe Ertekin | Yunus Emre Kundakcı | Tolga Ertekin

Yayıncı:

Marmara University

Ana Başlıklar:

Emergency Medicine

Dergi:

Clinical and Experimental Health Sciences Vol.13 Issue 4, pp.889-895

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001298491

pISSN:

2459-1459

Anahtar kelimeler:

Özet:

 Objective: Neural tube defects are one of the congenital malformations of the central nervous system. Although the factors that cause the development of neural tube defects and their mechanisms of action are still not clearly explained, genetic predisposition, drug use and some environmental factors are thought to play a role. In this study, it was aimed to investigate the effects of zuclopenthixol acetate ZA on neural tube development in a chick embryo model. Methods: Fourty specific pathogen-free SPF eggs were used in the study. The eggs were incubated for 28 hours and divided into four groups of 10 eggs each. At the end of the 28th hours, saline was injected to the control group, while ZA was administered subblastodermically to the experimental groups in 3 different doses 0.7, 1.4, 2.1 mgkg. At the end of the 48th hours, all the eggs were opened and the embryos were dissected from the embryonic membranes and evaluated morphologically and histopathologically. Results: When the study groups were evaluated according to the neural tube positions open or closed, it was found that the neural tube patency increased depending on the ZA dose, which was statistically significant p  0.05. In addition, morphological developments of embryos were evaluated. Compared to the control group, a statistically significant decrease was observed in the mean somite numbers in all ZA-treated groups, while a significant decrease was found in the mean cranio-caudal length only in the high-dose group. Conclusion: In this study, it was observed that neural tube and morphological development were adversely affected in the groups treated with ZA in the chick embryo model. It was shown that neural tube closure defects in embryos increased in direct proportion with ZA doses. However, we believe that it will not be possible to fully adapt the results of this study, which was carried out in the chick embryo model, to humans and that more comprehensive research should be conducted.

 Objective: Neural tube defects are one of the congenital malformations of the central nervous system. Although the factors that cause the development of neural tube defects and their mechanisms of action are still not clearly explained, genetic predisposition, drug use and some environmental factors are thought to play a role. In this study, it was aimed to investigate the effects of zuclopenthixol acetate ZA on neural tube development in a chick embryo model. Methods: Fourty specific pathogen-free SPF eggs were used in the study. The eggs were incubated for 28 hours and divided into four groups of 10 eggs each. At the end of the 28th hours, saline was injected to the control group, while ZA was administered subblastodermically to the experimental groups in 3 different doses 0.7, 1.4, 2.1 mgkg. At the end of the 48th hours, all the eggs were opened and the embryos were dissected from the embryonic membranes and evaluated morphologically and histopathologically. Results: When the study groups were evaluated according to the neural tube positions open or closed, it was found that the neural tube patency increased depending on the ZA dose, which was statistically significant p  0.05. In addition, morphological developments of embryos were evaluated. Compared to the control group, a statistically significant decrease was observed in the mean somite numbers in all ZA-treated groups, while a significant decrease was found in the mean cranio-caudal length only in the high-dose group. Conclusion: In this study, it was observed that neural tube and morphological development were adversely affected in the groups treated with ZA in the chick embryo model. It was shown that neural tube closure defects in embryos increased in direct proportion with ZA doses. However, we believe that it will not be possible to fully adapt the results of this study, which was carried out in the chick embryo model, to humans and that more comprehensive research should be conducted.

 Objective: Neural tube defects are one of the congenital malformations of the central nervous system. Although the factors that cause the development of neural tube defects and their mechanisms of action are still not clearly explained, genetic predisposition, drug use and some environmental factors are thought to play a role. In this study, it was aimed to investigate the effects of zuclopenthixol acetate ZA on neural tube development in a chick embryo model. Methods: Fourty specific pathogen-free SPF eggs were used in the study. The eggs were incubated for 28 hours and divided into four groups of 10 eggs each. At the end of the 28th hours, saline was injected to the control group, while ZA was administered subblastodermically to the experimental groups in 3 different doses 0.7, 1.4, 2.1 mgkg. At the end of the 48th hours, all the eggs were opened and the embryos were dissected from the embryonic membranes and evaluated morphologically and histopathologically. Results: When the study groups were evaluated according to the neural tube positions open or closed, it was found that the neural tube patency increased depending on the ZA dose, which was statistically significant p  0.05. In addition, morphological developments of embryos were evaluated. Compared to the control group, a statistically significant decrease was observed in the mean somite numbers in all ZA-treated groups, while a significant decrease was found in the mean cranio-caudal length only in the high-dose group. Conclusion: In this study, it was observed that neural tube and morphological development were adversely affected in the groups treated with ZA in the chick embryo model. It was shown that neural tube closure defects in embryos increased in direct proportion with ZA doses. However, we believe that it will not be possible to fully adapt the results of this study, which was carried out in the chick embryo model, to humans and that more comprehensive research should be conducted.

 Objective: Neural tube defects are one of the congenital malformations of the central nervous system. Although the factors that cause the development of neural tube defects and their mechanisms of action are still not clearly explained, genetic predisposition, drug use and some environmental factors are thought to play a role. In this study, it was aimed to investigate the effects of zuclopenthixol acetate ZA on neural tube development in a chick embryo model. Methods: Fourty specific pathogen-free SPF eggs were used in the study. The eggs were incubated for 28 hours and divided into four groups of 10 eggs each. At the end of the 28th hours, saline was injected to the control group, while ZA was administered subblastodermically to the experimental groups in 3 different doses 0.7, 1.4, 2.1 mgkg. At the end of the 48th hours, all the eggs were opened and the embryos were dissected from the embryonic membranes and evaluated morphologically and histopathologically. Results: When the study groups were evaluated according to the neural tube positions open or closed, it was found that the neural tube patency increased depending on the ZA dose, which was statistically significant p  0.05. In addition, morphological developments of embryos were evaluated. Compared to the control group, a statistically significant decrease was observed in the mean somite numbers in all ZA-treated groups, while a significant decrease was found in the mean cranio-caudal length only in the high-dose group. Conclusion: In this study, it was observed that neural tube and morphological development were adversely affected in the groups treated with ZA in the chick embryo model. It was shown that neural tube closure defects in embryos increased in direct proportion with ZA doses. However, we believe that it will not be possible to fully adapt the results of this study, which was carried out in the chick embryo model, to humans and that more comprehensive research should be conducted.

Makale detayları

Effect of Zuclopenthixol Acetate on Neural Tube Development in Early Chick Embryos

Yazarlar:

Gülan Albaş Kurt | Alperen Sarıtaş | Emre Atay | Ayşe Ertekin | Yunus Emre Kundakcı | Tolga Ertekin

Yayıncı:

Marmara University

Ana Başlıklar:

Emergency Medicine

Dergi:

Clinical and Experimental Health Sciences Vol.13 Issue 4, pp.889-895

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001298491

pISSN:

2459-1459

Chick embryo

congenital malformations

neural tube defect

zuclopenthixol acetateChick embryo

congenital malformations

neural tube defect

zuclopenthixol acetate

Sayı

Makale detayları

Yazarlar: Gülan Albaş Kurt | Alperen Sarıtaş | Emre Atay | Ayşe Ertekin | Yunus Emre Kundakcı | Tolga Ertekin

Yayıncı: Marmara University

Ana Başlıklar: Emergency Medicine

Dergi: Clinical and Experimental Health Sciences Vol.13 Issue 4, pp.889-895

Belge Tipi: Makale

Yayın Tarihi: 12/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001298491

pISSN: 2459-1459

Sayı

Yazarlar:

Gülan Albaş Kurt | Alperen Sarıtaş | Emre Atay | Ayşe Ertekin | Yunus Emre Kundakcı | Tolga Ertekin

Yayıncı:

Marmara University

Ana Başlıklar:

Emergency Medicine

Dergi:

Clinical and Experimental Health Sciences Vol.13 Issue 4, pp.889-895

Belge Tipi:

Makale

Yayın Tarihi:

12/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001298491

pISSN:

2459-1459