Distribution and Antibiotic Susceptibility of Bacteria Isolated from Urine Culture

Nurullah Çiftçi

Makale detayları

Distribution and Antibiotic Susceptibility of Bacteria Isolated from Urine Culture

Yazarlar:

Nurullah Çiftçi

Yayıncı:

Selcuk University

Ana Başlıklar:

Klinik Mikrobiyoloji

Dergi:

Genel Tıp Dergisi Vol.35 Issue 3, pp.471-475

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001351924

pISSN:

2602-3741

Anahtar kelimeler:

Özet:

 BackgroundAims: Empiric appropriate antibiotic therapy is recommended in patients with symptoms of urinary tract infectionsUTIs as it reduces the morbidity rate. Inappropriate antibiotic use leads to a decrease in treatment effectiveness and the development of late-stage complications. The aim of this study is retrospectively evaluate the results of urine cultures from patients prediagnosed with UTIs and the antibiotic resistance rate of the bacteria. Methods: Midstream urine samples from the patients were collected in sterile screw-cap containers and transported to the laboratory following standard procedures to avoid contamination. The urine samples were cultured on appropriate media and incubated at 37C for 24-48 hours. Bacterial identification was performed using conventional microbiological techniques. Antibiotic susceptibility testing was performed using the Kirby-Bauer disk diffusion method according to European Committee on Antimicrobial Susceptibility Testing guidelines. Results: In our study, a total of 1357 urine cultures were found to be positive for bacterial growth over one year. 1249 samples were obtained from adults, and 108 from children. The most frequently identified uropathogen in both adults and children was E. coli, with an average occurrence of 57.3. The second most frequently isolated bacteria were Enterococcus spp. in adults and Klebsiella spp. in children. In adults, the most resistant antibiotics were found to be ampicillin77.2 and amoxicillin-clavulanic acid 63.3, while the most sensitive antibiotics were carbapenems1. The highest resistance rate in P. aeruginosa strains were observed against ceftazidime38.9, while all strains were susceptible for colistin, tigecycline, and amikacin. Conclusions: The findings of this study underscore the importance of performing routine urine cultures to identify the specific pathogens causing UTIs and their susceptibility to antibiotics. Empirical treatment based on local antibiogram data is essential to ensure effective management and reduce unnecessary antibiotic use.

 BackgroundAims: Empiric appropriate antibiotic therapy is recommended in patients with symptoms of urinary tract infectionsUTIs as it reduces the morbidity rate. Inappropriate antibiotic use leads to a decrease in treatment effectiveness and the development of late-stage complications. The aim of this study is retrospectively evaluate the results of urine cultures from patients prediagnosed with UTIs and the antibiotic resistance rate of the bacteria. Methods: Midstream urine samples from the patients were collected in sterile screw-cap containers and transported to the laboratory following standard procedures to avoid contamination. The urine samples were cultured on appropriate media and incubated at 37C for 24-48 hours. Bacterial identification was performed using conventional microbiological techniques. Antibiotic susceptibility testing was performed using the Kirby-Bauer disk diffusion method according to European Committee on Antimicrobial Susceptibility Testing guidelines. Results: In our study, a total of 1357 urine cultures were found to be positive for bacterial growth over one year. 1249 samples were obtained from adults, and 108 from children. The most frequently identified uropathogen in both adults and children was E. coli, with an average occurrence of 57.3. The second most frequently isolated bacteria were Enterococcus spp. in adults and Klebsiella spp. in children. In adults, the most resistant antibiotics were found to be ampicillin77.2 and amoxicillin-clavulanic acid 63.3, while the most sensitive antibiotics were carbapenems1. The highest resistance rate in P. aeruginosa strains were observed against ceftazidime38.9, while all strains were susceptible for colistin, tigecycline, and amikacin. Conclusions: The findings of this study underscore the importance of performing routine urine cultures to identify the specific pathogens causing UTIs and their susceptibility to antibiotics. Empirical treatment based on local antibiogram data is essential to ensure effective management and reduce unnecessary antibiotic use.

 BackgroundAims: Empiric appropriate antibiotic therapy is recommended in patients with symptoms of urinary tract infectionsUTIs as it reduces the morbidity rate. Inappropriate antibiotic use leads to a decrease in treatment effectiveness and the development of late-stage complications. The aim of this study is retrospectively evaluate the results of urine cultures from patients prediagnosed with UTIs and the antibiotic resistance rate of the bacteria. Methods: Midstream urine samples from the patients were collected in sterile screw-cap containers and transported to the laboratory following standard procedures to avoid contamination. The urine samples were cultured on appropriate media and incubated at 37C for 24-48 hours. Bacterial identification was performed using conventional microbiological techniques. Antibiotic susceptibility testing was performed using the Kirby-Bauer disk diffusion method according to European Committee on Antimicrobial Susceptibility Testing guidelines. Results: In our study, a total of 1357 urine cultures were found to be positive for bacterial growth over one year. 1249 samples were obtained from adults, and 108 from children. The most frequently identified uropathogen in both adults and children was E. coli, with an average occurrence of 57.3. The second most frequently isolated bacteria were Enterococcus spp. in adults and Klebsiella spp. in children. In adults, the most resistant antibiotics were found to be ampicillin77.2 and amoxicillin-clavulanic acid 63.3, while the most sensitive antibiotics were carbapenems1. The highest resistance rate in P. aeruginosa strains were observed against ceftazidime38.9, while all strains were susceptible for colistin, tigecycline, and amikacin. Conclusions: The findings of this study underscore the importance of performing routine urine cultures to identify the specific pathogens causing UTIs and their susceptibility to antibiotics. Empirical treatment based on local antibiogram data is essential to ensure effective management and reduce unnecessary antibiotic use.

 Amaç: Semptomları bulunan idrar yolu enfeksiyonları İYE hastalarında ampirik uygun antibiyotik tedavisi önerilmektedir, çünkü bu, morbidite oranını azaltmaktadır. Uygunsuz antibiyotik kullanımı, tedavi etkinliğinde azalmaya ve geç evre komplikasyonlarının gelişmesine yol açmaktadır. Bu çalışmanın amacı, İYE tanısı konmuş hastalardan alınan idrar kültürlerinin sonuçlarını retrospektif olarak değerlendirmesi ve bakterilerin antibiyotik direnç oranlarını incelemektir. Materyal method: Hastalardan alınan orta akım idrar örnekleri, kontaminasyonu önlemek amacıyla standart prosedürlere uygun olarak steril vidalı kaplarda toplanmış ve laboratuvara taşınmıştır. İdrar örnekleri uygun besiyerlerine ekilip, 37Cde 24-48 saat inkübe edilmiştir. Bakteriyel tanı, konvansiyonel yöntemler kullanılarak yapılmıştır. Antibiyotik duyarlılık testi, EUCAST rehberinde ki önerilere göre Kirby-Bauer disk difüzyon yöntemi ile gerçekleştirilmiştir. Bulgular: Çalışmamızda, bir yıl boyunca 1357 idrar kültüründe bakteri üremesi saptanmıştır. Bu örneklerin 1249u yetişkinlerden, 108i ise çocuklardan alınan örneklerden üremiştir. Hem yetişkinlerde hem de çocuklarda en sık tespit edilen üropatogen Escherichia coli olup, ortalama izolasyon oranı 57,3 olarak hesaplanmıştır. Yetişkinlerde ikinci sırada Enterococcus spp., çocuklarda ise Klebsiella spp. izole edilmiştir. Yetişkinlerde en fazla direnç tespit edilen antibiyotikler ampisilin 77,2 ve amoksisilin-klavulanik asit 63,3 iken, en duyarlı antibiyotikler karbapenemler 1 olmuştur. Pseudomonas aeruginosa suşlarında en yüksek direnç oranı, ceftazidime 38,9 karşı gözlemlenmiş, ancak tüm suşlar kolistin, tigesiklin ve amikasin için duyarlı bulunmuştur. Sonuçlar: Bu çalışmanın bulguları, İYEye neden olan spesifik patojenlerin ve bunların antibiyotiklere duyarlılığının belirlenmesi için rutin idrar kültürlerinin yapılmasının önemini vurgulamaktadır. Lokal antibiyogram verilerine dayalı ampirik tedavi, etkin yönetimin sağlanması ve gereksiz antibiyotik kullanımının azaltılması için esastır.

Makale detayları

Distribution and Antibiotic Susceptibility of Bacteria Isolated from Urine Culture

Yazarlar:

Nurullah Çiftçi

Yayıncı:

Selcuk University

Ana Başlıklar:

Klinik Mikrobiyoloji

Dergi:

Genel Tıp Dergisi Vol.35 Issue 3, pp.471-475

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001351924

pISSN:

2602-3741

Antibacterial resistance

Carbapenems

Colistin

Escherichia coli

Urinary tract infectionAntibakteriyel direnç

Karbapenemler

Kolistin

Escherichia coli

İdrar yolu enfeksiyonu

Sayı

Makale detayları

Yazarlar: Nurullah Çiftçi

Yayıncı: Selcuk University

Ana Başlıklar: Klinik Mikrobiyoloji

Dergi: Genel Tıp Dergisi Vol.35 Issue 3, pp.471-475

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001351924

pISSN: 2602-3741

Sayı

Yazarlar:

Nurullah Çiftçi

Yayıncı:

Selcuk University

Ana Başlıklar:

Klinik Mikrobiyoloji

Dergi:

Genel Tıp Dergisi Vol.35 Issue 3, pp.471-475

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001351924

pISSN:

2602-3741