HISTOLOGICAL EFFECTS OF (4S)-2-(4-HYDROXY-3-METHOXYPHENYL) THIAZOLIDINE-4-CARBOXYLIC ACID ON ZEBRAFISH (Danio rerio) LIVER TISSUE

Nazan Deniz YÖN; Burcu ÖZTÜRK; Mustafa ZENGİN; Cansu AKBULUT

Makale detayları

HISTOLOGICAL EFFECTS OF (4S)-2-(4-HYDROXY-3-METHOXYPHENYL) THIAZOLIDINE-4-CARBOXYLIC ACID ON ZEBRAFISH (Danio rerio) LIVER TISSUE

Yazarlar:

Nazan Deniz YÖN | Burcu ÖZTÜRK | Mustafa ZENGİN | Cansu AKBULUT

Yayıncı:

Engin TUTKUN

Dergi:

The Turkish Journal Of Occupational / Environmental Medicine and Safety 2017 Vol.Volume 2 Issue İssue 1 (1), pp.1-176

Belge Tipi:

Makale

Yayın Tarihi:

2/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000835362

Sıklık:

Üç Aylık

eISSN:

2149-4711

pISSN:

2149-4711

Özet:

 


Examination the
histopathological effects of (4s)-2-(4-hydroxy-3-methoxyphenyl)
thiazolidine-4-carboxylic acid on liver tissue of zebrafish were aimed.

 (4S)-2-(4-hydroxy-3-methoxyphenyl)
thiazolidine-4-carboxylic acid is new synthesized substance which obtained from
cysteine and valine. Because of thiazolidine derivates have important
biological responses scientist work intensively on these compounds recent
years. It is obvious that thiazolidine contained compounds will be used in
future in the pharmaceutical industry to treat important diseases. 

After one-week
adaptation period zebrafish divided into four group (n=10) as one control and 3
experimental groups (0,2 mM, 0,4 mM, 0,6 mM). For investigating the effects of
thiazolidine, liver tissues were dissected after 5 day of the exposure. Tissues
were fixed with 10% neutral buffered formalin and dehydration were carried out
in an ascending series of ethanol. After tissues were cleared in xylene,
embedded in paraffin wax and cut into 5 µm sections on a microtome. The
sections were stained with hematoxylin (H&E) and PAS. Results were
evaluated with light microscope.








In control group
normal liver histology was monitored. Parenchyma cells, hepatocytes and their
cytoplasm were observed clearly. Sinusiods and nuclei were easily monitored. In
the 0,2 mM exposure group, degeneration, contraction at vacuoles in hepatocyte
cytoplasm, hypertrophy. Degeneration was detected at hepatocyte cells. In 0,4
mM exposure group, some kupffer cells were observed in elliptical form.
Degeneration and vacuolization were observed on kupffer cells. In 0,6 mM
exposure group, vacuolization at vacuoles in hepatocyte cytoplasm were
monitored. Vacuolization and degeneration were also observed hepatocyte
cells.  Vascular and sinusoidal
degeneration and steatozis were detected as well. As a result, with this study,
it is proved that (4s)-2-(4-hydroxy-3-methoxyphenyl) thiazolidine-4-carboxylic
acid cause deterioration in fish liver tissues.

.

Makale detayları

HISTOLOGICAL EFFECTS OF (4S)-2-(4-HYDROXY-3-METHOXYPHENYL) THIAZOLIDINE-4-CARBOXYLIC ACID ON ZEBRAFISH (Danio rerio) LIVER TISSUE

Yazarlar: Nazan Deniz YÖN | Burcu ÖZTÜRK | Mustafa ZENGİN | Cansu AKBULUT

Yayıncı: Engin TUTKUN

Dergi: The Turkish Journal Of Occupational / Environmental Medicine and Safety 2017 Vol.Volume 2 Issue İssue 1 (1), pp.1-176

Belge Tipi: Makale

Yayın Tarihi: 2/2017

Belge Dili: Türkçe

Document ID (Org No.): 2000835362

Sıklık: Üç Aylık

eISSN: 2149-4711

pISSN: 2149-4711

Sayı

Yazarlar:

Nazan Deniz YÖN | Burcu ÖZTÜRK | Mustafa ZENGİN | Cansu AKBULUT

Yayıncı:

Engin TUTKUN

Dergi:

The Turkish Journal Of Occupational / Environmental Medicine and Safety 2017 Vol.Volume 2 Issue İssue 1 (1), pp.1-176

Belge Tipi:

Makale

Yayın Tarihi:

2/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000835362

Sıklık:

Üç Aylık

eISSN:

2149-4711

pISSN:

2149-4711