Molecular Characterisation of Some Corn (Zea mays L.) Genotypes Growing in Iğdır Province

Barış Eren

Makale detayları

Molecular Characterisation of Some Corn (Zea mays L.) Genotypes Growing in Iğdır Province

Yazarlar:

Barış Eren

Yayıncı:

Igdir University

Ana Başlıklar:

Bitki Hücresi ve Moleküler Biyoloji | Tarımda Bitki Islahı

Dergi:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.14 Issue 4, pp.1731-1741

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001331759

pISSN:

2536-4618

Anahtar kelimeler:

Özet:

 Inter-primer binding site markers based on retrotransposons or iPBS retrotransposon markers have been useful in determining genetic diversity in a large number of organisms. The CTAB technique was employed to isolate DNA, and iPBS molecular markers were employed to conduct genetic diversity studies. Based on the analyses findings, the genotypes exhibited a significant degree of genetic diversity, with a 100 polymorphic locus percentage. A total of 154 polymorphic bands were generated as a consequence of molecular identification experiments conducted with 12 IPBS markers. The typical polymorphism rate was determined to be 100. Additionally, the average polymorphism PIC value, which quantifies the gene diversity of all markers examined in the study, was 0.228, and the average H value was 0.274. The genotypes 1 and 14 exhibited the lowest similarity ratio, with a coefficient value of 0.1600, when the DICE similarity coefficients were compared. These results were derived by comparing the DICE similarity coefficients of the samples. The analysis determined that the greatest similarity value between genotypes 4 and 3 was 0.6747. Maize genotypes are classified into four distinct subpopulations. Genotypes can be developed for reproductive research in order to demonstrate the molecular diversity of a population. It was determined that IPBS molecular markers are appropriate genetic instruments for genetic and phylogenetic analyses in maize varieties. This conclusion was attained as a consequence of the aforementioned. The data that is gathered will serve as a scientific foundation and a valuable contribution to the field of maize genetics in the future.

 Inter-primer binding site markers based on retrotransposons or iPBS retrotransposon markers have been useful in determining genetic diversity in a large number of organisms. The CTAB technique was employed to isolate DNA, and iPBS molecular markers were employed to conduct genetic diversity studies. Based on the analyses findings, the genotypes exhibited a significant degree of genetic diversity, with a 100 polymorphic locus percentage. A total of 154 polymorphic bands were generated as a consequence of molecular identification experiments conducted with 12 IPBS markers. The typical polymorphism rate was determined to be 100. Additionally, the average polymorphism PIC value, which quantifies the gene diversity of all markers examined in the study, was 0.228, and the average H value was 0.274. The genotypes 1 and 14 exhibited the lowest similarity ratio, with a coefficient value of 0.1600, when the DICE similarity coefficients were compared. These results were derived by comparing the DICE similarity coefficients of the samples. The analysis determined that the greatest similarity value between genotypes 4 and 3 was 0.6747. Maize genotypes are classified into four distinct subpopulations. Genotypes can be developed for reproductive research in order to demonstrate the molecular diversity of a population. It was determined that IPBS molecular markers are appropriate genetic instruments for genetic and phylogenetic analyses in maize varieties. This conclusion was attained as a consequence of the aforementioned. The data that is gathered will serve as a scientific foundation and a valuable contribution to the field of maize genetics in the future.

 Inter-primer binding site markers based on retrotransposons or iPBS retrotransposon markers have been useful in determining genetic diversity in a large number of organisms. The CTAB technique was employed to isolate DNA, and iPBS molecular markers were employed to conduct genetic diversity studies. Based on the analyses findings, the genotypes exhibited a significant degree of genetic diversity, with a 100 polymorphic locus percentage. A total of 154 polymorphic bands were generated as a consequence of molecular identification experiments conducted with 12 IPBS markers. The typical polymorphism rate was determined to be 100. Additionally, the average polymorphism PIC value, which quantifies the gene diversity of all markers examined in the study, was 0.228, and the average H value was 0.274. The genotypes 1 and 14 exhibited the lowest similarity ratio, with a coefficient value of 0.1600, when the DICE similarity coefficients were compared. These results were derived by comparing the DICE similarity coefficients of the samples. The analysis determined that the greatest similarity value between genotypes 4 and 3 was 0.6747. Maize genotypes are classified into four distinct subpopulations. Genotypes can be developed for reproductive research in order to demonstrate the molecular diversity of a population. It was determined that IPBS molecular markers are appropriate genetic instruments for genetic and phylogenetic analyses in maize varieties. This conclusion was attained as a consequence of the aforementioned. The data that is gathered will serve as a scientific foundation and a valuable contribution to the field of maize genetics in the future.

 Retrotranspozonlara dayalı primerler arası bağlanma bölgesi veya iPBS retrotranspozon belirteçleri, çok sayıda organizmada genetik çeşitliliğin belirlenmesinde yararlı olmuştur. DNAyı izole etmek için CTAB tekniği kullanılmış ve genetik çeşitlilik çalışmaları yapmak için iPBS moleküler belirteçleri kullanılmıştır. Analizlerin bulgularına göre, genotipler 100 polimorfik lokus yüzdesi ile önemli derecede genetik çeşitlilik sergilemiştir. 12 IPBS markörü ile yürütülen moleküler tanımlama deneyleri sonucunda toplam 154 polimorfik bant üretilmiştir. Tipik polimorfizm oranı 100 olarak belirlenmiştir. Ayrıca, çalışmada incelenen tüm markörlerin gen çeşitliliğini ölçen ortalama polimorfizm PIC değeri 0,228 ve ortalama H değeri 0,274 olarak belirlenmiştir. DICE benzerlik katsayıları karşılaştırıldığında, 1 ve 14 numaralı genotipler 0,1600 katsayı değeri ile en düşük benzerlik oranını sergilemiştir. Bu sonuçlar, örneklerin DICE benzerlik katsayılarının karşılaştırılmasıyla elde edilmiştir. Analiz, 4 ve 3 numaralı genotipler arasındaki en büyük benzerlik değerinin 0.6747 olduğunu belirledi. Mısır genotipleri dört farklı alt popülasyon olarak sınıflandırılmıştır. Genotipler, bir popülasyonun moleküler çeşitliliğini göstermek amacıyla üreme araştırmaları için geliştirilebilir. IPBS moleküler markörlerinin mısır çeşitlerinde genetik ve filogenetik analizler için uygun genetik araçlar olduğu belirlenmiştir. Bu sonuca yukarıda bahsedilenlerin bir sonucu olarak ulaşılmıştır. Elde edilen veriler, gelecekte mısır genetiği alanına bilimsel bir temel ve değerli bir katkı olarak hizmet edecektir.

Makale detayları

Molecular Characterisation of Some Corn (Zea mays L.) Genotypes Growing in Iğdır Province

Yazarlar:

Barış Eren

Yayıncı:

Igdir University

Ana Başlıklar:

Bitki Hücresi ve Moleküler Biyoloji | Tarımda Bitki Islahı

Dergi:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.14 Issue 4, pp.1731-1741

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001331759

pISSN:

2536-4618

Molecular marker

Maize

Zea mays L.Moleküler markör

Mısır

Zea mays L.

Sayı

Makale detayları

Molecular Characterisation of Some Corn (Zea mays L.) Genotypes Growing in Iğdır Province

Yazarlar: Barış Eren

Yayıncı: Igdir University

Ana Başlıklar: Bitki Hücresi ve Moleküler Biyoloji | Tarımda Bitki Islahı

Dergi: Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.14 Issue 4, pp.1731-1741

Belge Tipi: Makale

Yayın Tarihi: 1/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001331759

pISSN: 2536-4618

Molecular marker

Maize

Zea mays L.Moleküler markör

Mısır

Zea mays L.

Sayı

Yazarlar:

Barış Eren

Yayıncı:

Igdir University

Ana Başlıklar:

Bitki Hücresi ve Moleküler Biyoloji | Tarımda Bitki Islahı

Dergi:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.14 Issue 4, pp.1731-1741

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001331759

pISSN:

2536-4618