Micropropagation of Muscari latifolium, an Endemic Plant of Türkiye

Nesrin Bozhan; Ahmet Onay

Makale detayları

Micropropagation of Muscari latifolium, an Endemic Plant of Türkiye

Yazarlar:

Nesrin Bozhan | Ahmet Onay

Yayıncı:

Bitlis Eren University

Ana Başlıklar:

Plant Biotechnology

Dergi:

Bitlis Eren Üniversitesi Fen Bilimleri Dergisi Vol.14 Issue 3, pp.1563-1580

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359251

pISSN:

2147-3129

Anahtar kelimeler:

Özet:

 This study aimed to develop a seed-based in vitro propagation protocol for Muscari latifolium to ensure large-scale plant production while minimizing the impact on wild populations. Utilizing seeds as initial explants offers a sustainable and non-destructive alternative to bulb-based methods, promoting genetic diversity and supporting conservation efforts. Sterilization was optimized using 20 Sodium hypochlorite NaOCl for 30 minutes, achieving 100 decontamination and 100 germination. The most effective bulblet induction was observed on MS medium supplemented with 1.0 mgl 6-Benzylaminopurine BAP, yielding the maximum average bulblet number 21.06 per explant and optimal bulblet length 0.71 cm, significantly outperforming other cytokinin treatments, including Kinetin KIN. In contrast, the combination of 1.0 mgl BAP and 1.0 mgl α-Naphthalene Acetic Acid NAA also demonstrated substantial bulblet production, albeit at a lower average number 5.60 per explant, but with enhanced bulblet diameter 0.69 cm and a notable rooting rate of 75. While BAP alone proved superior for shoot multiplication and bulblet induction, adding NAA improved bulblet morphology and rooting efficiency, emphasizing the importance of cytokinin-auxin synergy. Rooting was most successful 100 with 2 mgl indole-3-butyric Acid IBA, producing robust roots, while acclimatization achieved a 47 survival rate using a sterile peat-soil mixture. The developed protocol provides a reliable, non-destructive method for the ex situ conservation and horticultural production of M. latifolium, leveraging seeds to reduce the need for bulb collection. This approach holds promise for propagating other endangered geophytes with similar challenges.

 This study aimed to develop a seed-based in vitro propagation protocol for Muscari latifolium to ensure large-scale plant production while minimizing the impact on wild populations. Utilizing seeds as initial explants offers a sustainable and non-destructive alternative to bulb-based methods, promoting genetic diversity and supporting conservation efforts. Sterilization was optimized using 20 Sodium hypochlorite NaOCl for 30 minutes, achieving 100 decontamination and 100 germination. The most effective bulblet induction was observed on MS medium supplemented with 1.0 mgl 6-Benzylaminopurine BAP, yielding the maximum average bulblet number 21.06 per explant and optimal bulblet length 0.71 cm, significantly outperforming other cytokinin treatments, including Kinetin KIN. In contrast, the combination of 1.0 mgl BAP and 1.0 mgl α-Naphthalene Acetic Acid NAA also demonstrated substantial bulblet production, albeit at a lower average number 5.60 per explant, but with enhanced bulblet diameter 0.69 cm and a notable rooting rate of 75. While BAP alone proved superior for shoot multiplication and bulblet induction, adding NAA improved bulblet morphology and rooting efficiency, emphasizing the importance of cytokinin-auxin synergy. Rooting was most successful 100 with 2 mgl indole-3-butyric Acid IBA, producing robust roots, while acclimatization achieved a 47 survival rate using a sterile peat-soil mixture. The developed protocol provides a reliable, non-destructive method for the ex situ conservation and horticultural production of M. latifolium, leveraging seeds to reduce the need for bulb collection. This approach holds promise for propagating other endangered geophytes with similar challenges.

 This study aimed to develop a seed-based in vitro propagation protocol for Muscari latifolium to ensure large-scale plant production while minimizing the impact on wild populations. Utilizing seeds as initial explants offers a sustainable and non-destructive alternative to bulb-based methods, promoting genetic diversity and supporting conservation efforts. Sterilization was optimized using 20 Sodium hypochlorite NaOCl for 30 minutes, achieving 100 decontamination and 100 germination. The most effective bulblet induction was observed on MS medium supplemented with 1.0 mgl 6-Benzylaminopurine BAP, yielding the maximum average bulblet number 21.06 per explant and optimal bulblet length 0.71 cm, significantly outperforming other cytokinin treatments, including Kinetin KIN. In contrast, the combination of 1.0 mgl BAP and 1.0 mgl α-Naphthalene Acetic Acid NAA also demonstrated substantial bulblet production, albeit at a lower average number 5.60 per explant, but with enhanced bulblet diameter 0.69 cm and a notable rooting rate of 75. While BAP alone proved superior for shoot multiplication and bulblet induction, adding NAA improved bulblet morphology and rooting efficiency, emphasizing the importance of cytokinin-auxin synergy. Rooting was most successful 100 with 2 mgl indole-3-butyric Acid IBA, producing robust roots, while acclimatization achieved a 47 survival rate using a sterile peat-soil mixture. The developed protocol provides a reliable, non-destructive method for the ex situ conservation and horticultural production of M. latifolium, leveraging seeds to reduce the need for bulb collection. This approach holds promise for propagating other endangered geophytes with similar challenges.

 This study aimed to develop a seed-based in vitro propagation protocol for Muscari latifolium to ensure large-scale plant production while minimizing the impact on wild populations. Utilizing seeds as initial explants offers a sustainable and non-destructive alternative to bulb-based methods, promoting genetic diversity and supporting conservation efforts. Sterilization was optimized using 20 Sodium hypochlorite NaOCl for 30 minutes, achieving 100 decontamination and 100 germination. The most effective bulblet induction was observed on MS medium supplemented with 1.0 mgl 6-Benzylaminopurine BAP, yielding the maximum average bulblet number 21.06 per explant and optimal bulblet length 0.71 cm, significantly outperforming other cytokinin treatments, including Kinetin KIN. In contrast, the combination of 1.0 mgl BAP and 1.0 mgl α-Naphthalene Acetic Acid NAA also demonstrated substantial bulblet production, albeit at a lower average number 5.60 per explant, but with enhanced bulblet diameter 0.69 cm and a notable rooting rate of 75. While BAP alone proved superior for shoot multiplication and bulblet induction, adding NAA improved bulblet morphology and rooting efficiency, emphasizing the importance of cytokinin-auxin synergy. Rooting was most successful 100 with 2 mgl indole-3-butyric Acid IBA, producing robust roots, while acclimatization achieved a 47 survival rate using a sterile peat-soil mixture. The developed protocol provides a reliable, non-destructive method for the ex situ conservation and horticultural production of M. latifolium, leveraging seeds to reduce the need for bulb collection. This approach holds promise for propagating other endangered geophytes with similar challenges.

Makale detayları

Micropropagation of Muscari latifolium, an Endemic Plant of Türkiye

Yazarlar:

Nesrin Bozhan | Ahmet Onay

Yayıncı:

Bitlis Eren University

Ana Başlıklar:

Plant Biotechnology

Dergi:

Bitlis Eren Üniversitesi Fen Bilimleri Dergisi Vol.14 Issue 3, pp.1563-1580

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359251

pISSN:

2147-3129

Bulblet formation

Micropropagation

Muscari latifolium

Tissue culture

Sayı

Makale detayları

Micropropagation of Muscari latifolium, an Endemic Plant of Türkiye

Yazarlar: Nesrin Bozhan | Ahmet Onay

Yayıncı: Bitlis Eren University

Ana Başlıklar: Plant Biotechnology

Dergi: Bitlis Eren Üniversitesi Fen Bilimleri Dergisi Vol.14 Issue 3, pp.1563-1580

Belge Tipi: Makale

Yayın Tarihi: 9/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001359251

pISSN: 2147-3129

Bulblet formation

Micropropagation

Muscari latifolium

Tissue culture

Sayı

Yazarlar:

Nesrin Bozhan | Ahmet Onay

Yayıncı:

Bitlis Eren University

Ana Başlıklar:

Plant Biotechnology

Dergi:

Bitlis Eren Üniversitesi Fen Bilimleri Dergisi Vol.14 Issue 3, pp.1563-1580

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359251

pISSN:

2147-3129