Surface Acoustic Wave Induced Heat Knockdown of Caenorhabditis Elegans

Adem ÖZÇELİK

doi:https://doi.org/10.17350/HJSE19030000210

Makale detayları

Surface Acoustic Wave Induced Heat Knockdown of Caenorhabditis Elegans

Yazarlar:

Adem ÖZÇELİK

Yayıncı:

Hitit University

Ana Başlıklar:

Uygulamalı fizik

Dergi:

Hittite Journal of Science and Engineering 2021 Vol.8 Issue 1, pp.35-40

Belge Tipi:

Makale

Yayın Tarihi:

3/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000451322

Sıklık:

Üç Aylık

eISSN:

2148-4171

DOI:

https://doi.org/10.17350/HJSE19030000210

Özet:

 

Model organisms such as Caenorhabditis elegans are commonly used in discovering theunderlying mechanisms of human diseases. The field of microfluidics enables a plethora of tools for better studying these organisms. In this research, an effective and simple approach is demonstrated for thermally induced knockdown of Caenorhabditis elegans model organisms. To achieve this, surface acoustic waves are coupled to the fluid medium inside a microfluidic channel. Without active cooling, two pairs of propagating surface acoustic waves induce very rapid heating of the enclose fluid. By controlling the input power of the acoustic waves, temperature of the fluid is precisely adjusted, and induced reversible knockdown of Caenorhabditis elegans samples. Effective temperature window of acoustically induced thermal knockdown is characterized, and the tolerable number of knockdown and wake up cycles is studied. Viability of the Caenorhabditis elegans organisms is also evaluated after increasing numbers of knockdown cycles. Overall, a simple and effective method of worm immobilization method is demonstrated using acoustic waves in microfluidics.

Makale detayları

Surface Acoustic Wave Induced Heat Knockdown of Caenorhabditis Elegans

Yazarlar: Adem ÖZÇELİK

Yayıncı: Hitit University

Ana Başlıklar: Uygulamalı fizik

Dergi: Hittite Journal of Science and Engineering 2021 Vol.8 Issue 1, pp.35-40

Belge Tipi: Makale

Yayın Tarihi: 3/2021

Belge Dili: İngilizce

Document ID (Org No.): 2000451322

Sıklık: Üç Aylık

eISSN: 2148-4171

DOI: https://doi.org/10.17350/HJSE19030000210

Sayı

Yazarlar:

Adem ÖZÇELİK

Yayıncı:

Hitit University

Ana Başlıklar:

Uygulamalı fizik

Dergi:

Hittite Journal of Science and Engineering 2021 Vol.8 Issue 1, pp.35-40

Belge Tipi:

Makale

Yayın Tarihi:

3/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000451322

Sıklık:

Üç Aylık

eISSN:

2148-4171

DOI:

https://doi.org/10.17350/HJSE19030000210