In vitro effects of antibiofilm agents and antibiotics on coagulase-negative staphylococci

İsmail Öztürk; Yamaç Tekintaş

Makale detayları

In vitro effects of antibiofilm agents and antibiotics on coagulase-negative staphylococci

Yazarlar:

İsmail Öztürk | Yamaç Tekintaş

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Microbiology

Dergi:

Journal of Research in Pharmacy Vol.24 Issue 6, pp.821-832

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001348006

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 Coagulase negative staphylococci CoNS are important nosocomial pathogens that cause biofilm infections. Biofilm provides advantages for microorganisms to resist antibiotics and host immune systems. Considering the increased antibiotic resistance, alternative treatments are needed to combat biofilm infections. In the present study, the effects of antibiotics including gentamicin GEN, ciprofloxacin, doxycycline DOX, rifampicin RIF and antibiofilm agents including N-acetylcysteine NAC, ethylenediaminetetraaceticacid EDTA, nisin NIS, farnesol FAR on clinical CoNS biofilm and IS256, icaA gene expression levels were evaluated. Forty-five CoNS strains were isolated from patients catheters, at Manisa Celal Bayar University Hospital. The minimum inhibitory concentrations MICs of agents were detected by broth microdilution method with European Committee for Antimicrobial Susceptibility Testing EUCAST criteria. The combined effects of agents were investigated by checkerboard method. The antibiofilm effects of combinations were investigated by spectrophotometric microplate method. The effects of combinations on IS256 and icaA gene expressions were evaluated by real-time quantitative reverse-transcriptase PCR. Twenty-four isolates 53.3 were detected as strong biofilm producer. Biofilm production was inhibited in seven isolates in the presence of EDTARIF and NISDOX while NISGEN combination and RIF inhibited biofilm in six isolates. Nine combinations were found to have synergistic effect against isolate 6 which are resistant to four different antibiotics. The expressions of icaA and IS256 were downregulated in the presence of EDTA, NACCIP, NACGEN, NISGEN, FARGEN. Antibiofilm agentantimicrobial combinations could have promising effects for preventing catheter colonization. The further studies on antibiofilm treatment strategies would be beneficial for decreasing morbidity-mortality rates and healthcare costs caused by biofilms.

 Coagulase negative staphylococci CoNS are important nosocomial pathogens that cause biofilm infections. Biofilm provides advantages for microorganisms to resist antibiotics and host immune systems. Considering the increased antibiotic resistance, alternative treatments are needed to combat biofilm infections. In the present study, the effects of antibiotics including gentamicin GEN, ciprofloxacin, doxycycline DOX, rifampicin RIF and antibiofilm agents including N-acetylcysteine NAC, ethylenediaminetetraaceticacid EDTA, nisin NIS, farnesol FAR on clinical CoNS biofilm and IS256, icaA gene expression levels were evaluated. Forty-five CoNS strains were isolated from patients catheters, at Manisa Celal Bayar University Hospital. The minimum inhibitory concentrations MICs of agents were detected by broth microdilution method with European Committee for Antimicrobial Susceptibility Testing EUCAST criteria. The combined effects of agents were investigated by checkerboard method. The antibiofilm effects of combinations were investigated by spectrophotometric microplate method. The effects of combinations on IS256 and icaA gene expressions were evaluated by real-time quantitative reverse-transcriptase PCR. Twenty-four isolates 53.3 were detected as strong biofilm producer. Biofilm production was inhibited in seven isolates in the presence of EDTARIF and NISDOX while NISGEN combination and RIF inhibited biofilm in six isolates. Nine combinations were found to have synergistic effect against isolate 6 which are resistant to four different antibiotics. The expressions of icaA and IS256 were downregulated in the presence of EDTA, NACCIP, NACGEN, NISGEN, FARGEN. Antibiofilm agentantimicrobial combinations could have promising effects for preventing catheter colonization. The further studies on antibiofilm treatment strategies would be beneficial for decreasing morbidity-mortality rates and healthcare costs caused by biofilms.

 Coagulase negative staphylococci CoNS are important nosocomial pathogens that cause biofilm infections. Biofilm provides advantages for microorganisms to resist antibiotics and host immune systems. Considering the increased antibiotic resistance, alternative treatments are needed to combat biofilm infections. In the present study, the effects of antibiotics including gentamicin GEN, ciprofloxacin, doxycycline DOX, rifampicin RIF and antibiofilm agents including N-acetylcysteine NAC, ethylenediaminetetraaceticacid EDTA, nisin NIS, farnesol FAR on clinical CoNS biofilm and IS256, icaA gene expression levels were evaluated. Forty-five CoNS strains were isolated from patients catheters, at Manisa Celal Bayar University Hospital. The minimum inhibitory concentrations MICs of agents were detected by broth microdilution method with European Committee for Antimicrobial Susceptibility Testing EUCAST criteria. The combined effects of agents were investigated by checkerboard method. The antibiofilm effects of combinations were investigated by spectrophotometric microplate method. The effects of combinations on IS256 and icaA gene expressions were evaluated by real-time quantitative reverse-transcriptase PCR. Twenty-four isolates 53.3 were detected as strong biofilm producer. Biofilm production was inhibited in seven isolates in the presence of EDTARIF and NISDOX while NISGEN combination and RIF inhibited biofilm in six isolates. Nine combinations were found to have synergistic effect against isolate 6 which are resistant to four different antibiotics. The expressions of icaA and IS256 were downregulated in the presence of EDTA, NACCIP, NACGEN, NISGEN, FARGEN. Antibiofilm agentantimicrobial combinations could have promising effects for preventing catheter colonization. The further studies on antibiofilm treatment strategies would be beneficial for decreasing morbidity-mortality rates and healthcare costs caused by biofilms.

 Coagulase negative staphylococci CoNS are important nosocomial pathogens that cause biofilm infections. Biofilm provides advantages for microorganisms to resist antibiotics and host immune systems. Considering the increased antibiotic resistance, alternative treatments are needed to combat biofilm infections. In the present study, the effects of antibiotics including gentamicin GEN, ciprofloxacin, doxycycline DOX, rifampicin RIF and antibiofilm agents including N-acetylcysteine NAC, ethylenediaminetetraaceticacid EDTA, nisin NIS, farnesol FAR on clinical CoNS biofilm and IS256, icaA gene expression levels were evaluated. Forty-five CoNS strains were isolated from patients catheters, at Manisa Celal Bayar University Hospital. The minimum inhibitory concentrations MICs of agents were detected by broth microdilution method with European Committee for Antimicrobial Susceptibility Testing EUCAST criteria. The combined effects of agents were investigated by checkerboard method. The antibiofilm effects of combinations were investigated by spectrophotometric microplate method. The effects of combinations on IS256 and icaA gene expressions were evaluated by real-time quantitative reverse-transcriptase PCR. Twenty-four isolates 53.3 were detected as strong biofilm producer. Biofilm production was inhibited in seven isolates in the presence of EDTARIF and NISDOX while NISGEN combination and RIF inhibited biofilm in six isolates. Nine combinations were found to have synergistic effect against isolate 6 which are resistant to four different antibiotics. The expressions of icaA and IS256 were downregulated in the presence of EDTA, NACCIP, NACGEN, NISGEN, FARGEN. Antibiofilm agentantimicrobial combinations could have promising effects for preventing catheter colonization. The further studies on antibiofilm treatment strategies would be beneficial for decreasing morbidity-mortality rates and healthcare costs caused by biofilms.

Makale detayları

In vitro effects of antibiofilm agents and antibiotics on coagulase-negative staphylococci

Yazarlar:

İsmail Öztürk | Yamaç Tekintaş

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Microbiology

Dergi:

Journal of Research in Pharmacy Vol.24 Issue 6, pp.821-832

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001348006

pISSN:

2630-6344

Coagulase negative staphylococci

biofilm production

antibiofilm agents

synergistic effect

gene expression

Sayı

Makale detayları

In vitro effects of antibiofilm agents and antibiotics on coagulase-negative staphylococci

Yazarlar: İsmail Öztürk | Yamaç Tekintaş

Yayıncı: Marmara University

Ana Başlıklar: Pharmaceutical Microbiology

Dergi: Journal of Research in Pharmacy Vol.24 Issue 6, pp.821-832

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001348006

pISSN: 2630-6344

Coagulase negative staphylococci

biofilm production

antibiofilm agents

synergistic effect

gene expression

Sayı

Yazarlar:

İsmail Öztürk | Yamaç Tekintaş

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Microbiology

Dergi:

Journal of Research in Pharmacy Vol.24 Issue 6, pp.821-832

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001348006

pISSN:

2630-6344