Preparation and In Vitro Characterization of Carbamazepine-Loaded Chitosan-Coated/Uncoated PLGA and Zein Nanoparticles

Afife Büşra Uğur Kaplan; Meltem Çetin

Makale detayları

Preparation and In Vitro Characterization of Carbamazepine-Loaded Chitosan-Coated/Uncoated PLGA and Zein Nanoparticles

Yazarlar:

Afife Büşra Uğur Kaplan | Meltem Çetin

Yayıncı:

Karyay Karadeniz Yayımcılık

Ana Başlıklar:

Nanobiyoteknoloji | Polimer Bilimi ve Teknolojileri

Dergi:

Black Sea Journal of Engineering and Science Vol.7 Issue 5, pp.874-881

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001324905

pISSN:

2619-8991

Anahtar kelimeler:

Özet:

 The aim of this study is to prepare CBZ-loaded chitosan Ch- coateduncoated polylactic-co-glycolic acid PLGA and Zein using 20 mg or 40 mg Zein nanoparticles CBZ-PLGA-Zein-NPs or CBZ-PLGA-Zein-Ch-NPs and to characterize Particle size, PDI, zeta potential, percent encapsulation efficiency EE, FT-IR, DSC and XRD analyzes, and in vitro release study them in vitro. These nanoparticles were prepared using a modified emulsification-solvent evaporation method. The particle sizes of CBZ-PLGA-Zein20-NPs, CBZ-PLGA-Zein40-NPs and CBZ-PLGA-Zein20-Ch-NPs were found to be about 222 nm, 245 nm and 221 nm, respectively. The PDI value of all NP formulations was below 0.3. This indicates a narrow particle size distribution. The EE values of CBZ-PLGA-Zein20-NPs, CBZ-PLGA-Zein40-NPs and CBZ-PLGA-Zein20-Ch-NPs were determined as about 64, 56 and 62, respectively. The coating of the optimum formulation containing 20 mg Zein with chitosan did not lead to a significant difference in the particle size and EE value of this formulation P0.05. A sustained release of CBZ from all prepared NPs formulations was achieved until 48th h. In conclusion, CBZ-PLGA-Zein20 mg or 40 mg-NPs and CBZ-PLGA-Zein20 mg-Ch-NPs were successfully prepared and characterized in vitro.

 The aim of this study is to prepare CBZ-loaded chitosan Ch- coateduncoated polylactic-co-glycolic acid PLGA and Zein using 20 mg or 40 mg Zein nanoparticles CBZ-PLGA-Zein-NPs or CBZ-PLGA-Zein-Ch-NPs and to characterize Particle size, PDI, zeta potential, percent encapsulation efficiency EE, FT-IR, DSC and XRD analyzes, and in vitro release study them in vitro. These nanoparticles were prepared using a modified emulsification-solvent evaporation method. The particle sizes of CBZ-PLGA-Zein20-NPs, CBZ-PLGA-Zein40-NPs and CBZ-PLGA-Zein20-Ch-NPs were found to be about 222 nm, 245 nm and 221 nm, respectively. The PDI value of all NP formulations was below 0.3. This indicates a narrow particle size distribution. The EE values of CBZ-PLGA-Zein20-NPs, CBZ-PLGA-Zein40-NPs and CBZ-PLGA-Zein20-Ch-NPs were determined as about 64, 56 and 62, respectively. The coating of the optimum formulation containing 20 mg Zein with chitosan did not lead to a significant difference in the particle size and EE value of this formulation P0.05. A sustained release of CBZ from all prepared NPs formulations was achieved until 48th h. In conclusion, CBZ-PLGA-Zein20 mg or 40 mg-NPs and CBZ-PLGA-Zein20 mg-Ch-NPs were successfully prepared and characterized in vitro.

 The aim of this study is to prepare CBZ-loaded chitosan Ch- coateduncoated polylactic-co-glycolic acid PLGA and Zein using 20 mg or 40 mg Zein nanoparticles CBZ-PLGA-Zein-NPs or CBZ-PLGA-Zein-Ch-NPs and to characterize Particle size, PDI, zeta potential, percent encapsulation efficiency EE, FT-IR, DSC and XRD analyzes, and in vitro release study them in vitro. These nanoparticles were prepared using a modified emulsification-solvent evaporation method. The particle sizes of CBZ-PLGA-Zein20-NPs, CBZ-PLGA-Zein40-NPs and CBZ-PLGA-Zein20-Ch-NPs were found to be about 222 nm, 245 nm and 221 nm, respectively. The PDI value of all NP formulations was below 0.3. This indicates a narrow particle size distribution. The EE values of CBZ-PLGA-Zein20-NPs, CBZ-PLGA-Zein40-NPs and CBZ-PLGA-Zein20-Ch-NPs were determined as about 64, 56 and 62, respectively. The coating of the optimum formulation containing 20 mg Zein with chitosan did not lead to a significant difference in the particle size and EE value of this formulation P0.05. A sustained release of CBZ from all prepared NPs formulations was achieved until 48th h. In conclusion, CBZ-PLGA-Zein20 mg or 40 mg-NPs and CBZ-PLGA-Zein20 mg-Ch-NPs were successfully prepared and characterized in vitro.

 The aim of this study is to prepare CBZ-loaded chitosan Ch- coateduncoated polylactic-co-glycolic acid PLGA and Zein using 20 mg or 40 mg Zein nanoparticles CBZ-PLGA-Zein-NPs or CBZ-PLGA-Zein-Ch-NPs and to characterize Particle size, PDI, zeta potential, percent encapsulation efficiency EE, FT-IR, DSC and XRD analyzes, and in vitro release study them in vitro. These nanoparticles were prepared using a modified emulsification-solvent evaporation method. The particle sizes of CBZ-PLGA-Zein20-NPs, CBZ-PLGA-Zein40-NPs and CBZ-PLGA-Zein20-Ch-NPs were found to be about 222 nm, 245 nm and 221 nm, respectively. The PDI value of all NP formulations was below 0.3. This indicates a narrow particle size distribution. The EE values of CBZ-PLGA-Zein20-NPs, CBZ-PLGA-Zein40-NPs and CBZ-PLGA-Zein20-Ch-NPs were determined as about 64, 56 and 62, respectively. The coating of the optimum formulation containing 20 mg Zein with chitosan did not lead to a significant difference in the particle size and EE value of this formulation P0.05. A sustained release of CBZ from all prepared NPs formulations was achieved until 48th h. In conclusion, CBZ-PLGA-Zein20 mg or 40 mg-NPs and CBZ-PLGA-Zein20 mg-Ch-NPs were successfully prepared and characterized in vitro.

Makale detayları

Preparation and In Vitro Characterization of Carbamazepine-Loaded Chitosan-Coated/Uncoated PLGA and Zein Nanoparticles

Yazarlar:

Afife Büşra Uğur Kaplan | Meltem Çetin

Yayıncı:

Karyay Karadeniz Yayımcılık

Ana Başlıklar:

Nanobiyoteknoloji | Polimer Bilimi ve Teknolojileri

Dergi:

Black Sea Journal of Engineering and Science Vol.7 Issue 5, pp.874-881

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001324905

pISSN:

2619-8991

Epilepsy

Chitosan

Nanoparticles

PLGA

ZeinEpilepsy

Chitosan

Nanoparticles

PLGA

Zein

Sayı

Makale detayları

Yazarlar: Afife Büşra Uğur Kaplan | Meltem Çetin

Yayıncı: Karyay Karadeniz Yayımcılık

Ana Başlıklar: Nanobiyoteknoloji | Polimer Bilimi ve Teknolojileri

Dergi: Black Sea Journal of Engineering and Science Vol.7 Issue 5, pp.874-881

Belge Tipi: Makale

Yayın Tarihi: 9/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001324905

pISSN: 2619-8991

Sayı

Yazarlar:

Afife Büşra Uğur Kaplan | Meltem Çetin

Yayıncı:

Karyay Karadeniz Yayımcılık

Ana Başlıklar:

Nanobiyoteknoloji | Polimer Bilimi ve Teknolojileri

Dergi:

Black Sea Journal of Engineering and Science Vol.7 Issue 5, pp.874-881

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001324905

pISSN:

2619-8991