Investigating the Effect of Yttrium Oxide Nanoparticle in U87MG Glioma and PC3 Prostate Cancer: Molecular Approaches

Elanur AYDIN KARATAŞ; Kübra BAYINDIRLI; Özlem ÖZDEMİR TOZLU; Erdal SÖNMEZ; Süleyman KERLİ; Hasan TÜRKEZ; Ayşenur YAZICI

doi:https://doi.org/10.21597/jist.872394

Makale detayları

Investigating the Effect of Yttrium Oxide Nanoparticle in U87MG Glioma and PC3 Prostate Cancer: Molecular Approaches

Yazarlar:

Elanur AYDIN KARATAŞ | Kübra BAYINDIRLI | Özlem ÖZDEMİR TOZLU | Erdal SÖNMEZ | Süleyman KERLİ | Hasan TÜRKEZ | Ayşenur YAZICI

Yayıncı:

Igdir University

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Journal of the Institute of Science and Technology 2021 Vol.11 Issue 3, pp.2307-2318

Belge Tipi:

Makale

Yayın Tarihi:

8/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000462672

Sıklık:

Üç Aylık

eISSN:

2536-4618

DOI:

https://doi.org/10.21597/jist.872394

Özet:

 

Yttrium oxide (Y2O3) nanoparticles have very wide application areas such as biological
imaging, photodynamic therapy, the material sciences, in the chemical synthesis of inorganic
compounds, additives in plastic, paint, steel, optics, and iron. Potential risks to human health and the
environment should be evaluated in a multi-dimensional perspective when developing nanoparticles for
those applications. Therefore, in this research, we aimed to investigate changes in gene expression
profiles (genes involved in different biological pathways) influenced by commonly Yttrium oxide
(Y2O3) nanoparticle in human U87MG glioma and PC3 prostate cancer cell lines in vitro. The study was
planned to be carried out in two stages. In the first stage, cell viability and cytotoxicity parameters were
studied using 3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide and lactate dehydrogenase
release assays, respectively, with human U87MG glioma and human PC3 prostate cancer cell cultures.
In the second stage, to obtain a clear insight into the molecular events after exposing, we examined the
effects of selected Y2O3 nanoparticle on the expression of genes in U87MG and PC3 cell cultures using
RT2 Profiler PCR Arrays. Y2O3 nanoparticles have IC20 of 0,18 mg/L and 2,903 mg/L in PC3 and
U87MG cell lines, respectively. Y2O3 nanoparticle induced up-regulation of 24 and down-regulation of
22 genes in PC3 cells and up-regulation of 53 and down-regulation of 27 genes in U87MG cells. This
study of gene expression profiles affected by nanotoxicity provides critical information for the clinical
and environmental applications of Y2O3 nanoparticles.

 

Yttrium oxide (Y2O3) nanoparticles have very wide application areas such as biological
imaging, photodynamic therapy, the material sciences, in the chemical synthesis of inorganic
compounds, additives in plastic, paint, steel, optics, and iron. Potential risks to human health and the
environment should be evaluated in a multi-dimensional perspective when developing nanoparticles for
those applications. Therefore, in this research, we aimed to investigate changes in gene expression
profiles (genes involved in different biological pathways) influenced by commonly Yttrium oxide
(Y2O3) nanoparticle in human U87MG glioma and PC3 prostate cancer cell lines in vitro. The study was
planned to be carried out in two stages. In the first stage, cell viability and cytotoxicity parameters were
studied using 3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide and lactate dehydrogenase
release assays, respectively, with human U87MG glioma and human PC3 prostate cancer cell cultures.
In the second stage, to obtain a clear insight into the molecular events after exposing, we examined the
effects of selected Y2O3 nanoparticle on the expression of genes in U87MG and PC3 cell cultures using
RT2 Profiler PCR Arrays. Y2O3 nanoparticles have IC20 of 0,18 mg/L and 2,903 mg/L in PC3 and
U87MG cell lines, respectively. Y2O3 nanoparticle induced up-regulation of 24 and down-regulation of
22 genes in PC3 cells and up-regulation of 53 and down-regulation of 27 genes in U87MG cells. This
study of gene expression profiles affected by nanotoxicity provides critical information for the clinical
and environmental applications of Y2O3 nanoparticles.

Makale detayları

Investigating the Effect of Yttrium Oxide Nanoparticle in U87MG Glioma and PC3 Prostate Cancer: Molecular Approaches

Yazarlar: Elanur AYDIN KARATAŞ | Kübra BAYINDIRLI | Özlem ÖZDEMİR TOZLU | Erdal SÖNMEZ | Süleyman KERLİ | Hasan TÜRKEZ | Ayşenur YAZICI

Yayıncı: Igdir University

Ana Başlıklar: Yaşam bilimleri

Dergi: Journal of the Institute of Science and Technology 2021 Vol.11 Issue 3, pp.2307-2318

Belge Tipi: Makale

Yayın Tarihi: 8/2021

Belge Dili: İngilizce

Document ID (Org No.): 2000462672

Sıklık: Üç Aylık

eISSN: 2536-4618

DOI: https://doi.org/10.21597/jist.872394

Sayı

Yazarlar:

Elanur AYDIN KARATAŞ | Kübra BAYINDIRLI | Özlem ÖZDEMİR TOZLU | Erdal SÖNMEZ | Süleyman KERLİ | Hasan TÜRKEZ | Ayşenur YAZICI

Yayıncı:

Igdir University

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Journal of the Institute of Science and Technology 2021 Vol.11 Issue 3, pp.2307-2318

Belge Tipi:

Makale

Yayın Tarihi:

8/2021

Belge Dili:

İngilizce

Document ID (Org No.):

2000462672

Sıklık:

Üç Aylık

eISSN:

2536-4618

DOI:

https://doi.org/10.21597/jist.872394