Evaluation of Neutrophil Extracellular Trap Formation on Smokers and Non-Smokers with Periodontitis

Gizem Yücesoy; Ebru Olgun; Kader Yıldız; Merve Başol Göksuluk

Makale detayları

Evaluation of Neutrophil Extracellular Trap Formation on Smokers and Non-Smokers with Periodontitis

Yazarlar:

Gizem Yücesoy | Ebru Olgun | Kader Yıldız | Merve Başol Göksuluk

Yayıncı:

Aydin Adnan Menderes University

Ana Başlıklar:

Dentistry (Other)

Dergi:

Meandros Medical And Dental Journal Vol.25 Issue 2, pp.195-208

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001325148

pISSN:

2149-9063

Anahtar kelimeler:

Özet:

 Objevtive: In this study, we aimed to evaluate neutrophil extracellular trap NET in smoker and non-smoker periodontitis patients. Materials and Methods: 21 subjects non-smokers and periodontally healthy S-P- 7 people, non-smokers with periodontitis S-P 7 people, smokers with periodontitis SP 7 people were included in this study. Plaque index PI, gingival index GI, probing depth PD and clinical attachment level CAL parameters were recorded. The peripheral neutrophils isolated from the subjects were activated by phorbol 12-myristate 13-acetate PMA to induce NET formation. NET formed after the activation was measured with the fluorometer. Results: In the S-P- group PI, GI, PD and CAL values were found statistically significantly low P0,001; P0,001; P0,003; P0,001, respectively. When compared to SP group PMN counts were found low, but this difference was statistically at the border P0,05. When compared NET count values, it was the highest in the SP group. When S-P- group and SP group were compared, this difference was statistically significant P0,003; but between S-P and SP groups, the difference was not statistically significant P0.05. NET count with PI, GI, PD, there was mild positive; with CAL strong positive relation has been found. Conclusion: As a result, even the SP group NET count was found higher, the difference of S-P and SP groups was not statistically significant. There is need more researches about smoking effect on NET formation in patients with periodontitis.

 Objevtive: In this study, we aimed to evaluate neutrophil extracellular trap NET in smoker and non-smoker periodontitis patients. Materials and Methods: 21 subjects non-smokers and periodontally healthy S-P- 7 people, non-smokers with periodontitis S-P 7 people, smokers with periodontitis SP 7 people were included in this study. Plaque index PI, gingival index GI, probing depth PD and clinical attachment level CAL parameters were recorded. The peripheral neutrophils isolated from the subjects were activated by phorbol 12-myristate 13-acetate PMA to induce NET formation. NET formed after the activation was measured with the fluorometer. Results: In the S-P- group PI, GI, PD and CAL values were found statistically significantly low P0,001; P0,001; P0,003; P0,001, respectively. When compared to SP group PMN counts were found low, but this difference was statistically at the border P0,05. When compared NET count values, it was the highest in the SP group. When S-P- group and SP group were compared, this difference was statistically significant P0,003; but between S-P and SP groups, the difference was not statistically significant P0.05. NET count with PI, GI, PD, there was mild positive; with CAL strong positive relation has been found. Conclusion: As a result, even the SP group NET count was found higher, the difference of S-P and SP groups was not statistically significant. There is need more researches about smoking effect on NET formation in patients with periodontitis.

 Objevtive: In this study, we aimed to evaluate neutrophil extracellular trap NET in smoker and non-smoker periodontitis patients. Materials and Methods: 21 subjects non-smokers and periodontally healthy S-P- 7 people, non-smokers with periodontitis S-P 7 people, smokers with periodontitis SP 7 people were included in this study. Plaque index PI, gingival index GI, probing depth PD and clinical attachment level CAL parameters were recorded. The peripheral neutrophils isolated from the subjects were activated by phorbol 12-myristate 13-acetate PMA to induce NET formation. NET formed after the activation was measured with the fluorometer. Results: In the S-P- group PI, GI, PD and CAL values were found statistically significantly low P0,001; P0,001; P0,003; P0,001, respectively. When compared to SP group PMN counts were found low, but this difference was statistically at the border P0,05. When compared NET count values, it was the highest in the SP group. When S-P- group and SP group were compared, this difference was statistically significant P0,003; but between S-P and SP groups, the difference was not statistically significant P0.05. NET count with PI, GI, PD, there was mild positive; with CAL strong positive relation has been found. Conclusion: As a result, even the SP group NET count was found higher, the difference of S-P and SP groups was not statistically significant. There is need more researches about smoking effect on NET formation in patients with periodontitis.

 Objevtive: In this study, we aimed to evaluate neutrophil extracellular trap NET in smoker and non-smoker periodontitis patients. Materials and Methods: 21 subjects non-smokers and periodontally healthy S-P- 7 people, non-smokers with periodontitis S-P 7 people, smokers with periodontitis SP 7 people were included in this study. Plaque index PI, gingival index GI, probing depth PD and clinical attachment level CAL parameters were recorded. The peripheral neutrophils isolated from the subjects were activated by phorbol 12-myristate 13-acetate PMA to induce NET formation. NET formed after the activation was measured with the fluorometer. Results: In the S-P- group PI, GI, PD and CAL values were found statistically significantly low P0,001; P0,001; P0,003; P0,001, respectively. When compared to SP group PMN counts were found low, but this difference was statistically at the border P0,05. When compared NET count values, it was the highest in the SP group. When S-P- group and SP group were compared, this difference was statistically significant P0,003; but between S-P and SP groups, the difference was not statistically significant P0.05. NET count with PI, GI, PD, there was mild positive; with CAL strong positive relation has been found. Conclusion: As a result, even the SP group NET count was found higher, the difference of S-P and SP groups was not statistically significant. There is need more researches about smoking effect on NET formation in patients with periodontitis.

Makale detayları

Evaluation of Neutrophil Extracellular Trap Formation on Smokers and Non-Smokers with Periodontitis

Yazarlar:

Gizem Yücesoy | Ebru Olgun | Kader Yıldız | Merve Başol Göksuluk

Yayıncı:

Aydin Adnan Menderes University

Ana Başlıklar:

Dentistry (Other)

Dergi:

Meandros Medical And Dental Journal Vol.25 Issue 2, pp.195-208

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001325148

pISSN:

2149-9063

Periodontitis

Smoking

Neutrophil Extracullular Trap

Sayı

Makale detayları

Evaluation of Neutrophil Extracellular Trap Formation on Smokers and Non-Smokers with Periodontitis

Yazarlar: Gizem Yücesoy | Ebru Olgun | Kader Yıldız | Merve Başol Göksuluk

Yayıncı: Aydin Adnan Menderes University

Ana Başlıklar: Dentistry (Other)

Dergi: Meandros Medical And Dental Journal Vol.25 Issue 2, pp.195-208

Belge Tipi: Makale

Yayın Tarihi: 8/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001325148

pISSN: 2149-9063

Periodontitis

Smoking

Neutrophil Extracullular Trap

Sayı

Yazarlar:

Gizem Yücesoy | Ebru Olgun | Kader Yıldız | Merve Başol Göksuluk

Yayıncı:

Aydin Adnan Menderes University

Ana Başlıklar:

Dentistry (Other)

Dergi:

Meandros Medical And Dental Journal Vol.25 Issue 2, pp.195-208

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001325148

pISSN:

2149-9063