Rho/rho-kinase signalling in chronically alcohol-fed mice

Havva KUBAT; Ahmet Sencer YURTSEVER; Kansu BÜYÜKAFŞAR

Makale detayları

Rho/rho-kinase signalling in chronically alcohol-fed mice

Yazarlar:

Havva KUBAT | Ahmet Sencer YURTSEVER | Kansu BÜYÜKAFŞAR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2017 Vol.47 Issue 2, pp.668-674

Belge Tipi:

Makale

Yayın Tarihi:

3/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000751492

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Özet:

 

Background/aim: The effects of chronic ethanol consumption on male sexual function are debateable, and its effects on the Rho/  Rho-kinase pathway are unknown. Therefore, we investigated smooth muscle reactivity and Rho/Rho-kinase signalling in the corpus  cavernosum of ethanol-fed mice.  Materials and methods: Ethanol was added to drinking water at 5% concentration in the first 2 days with 5% increment every  subsequent 2 days, up to a final concentration of 20% for 45 days. Corpora cavernosa were isolated and a cumulative dose-response  curve to phenylephrine was obtained. Acetylcholine (10-6 M), electrical field stimulation (EFS, 40 V, 8-16 Hz) and Y-27632 (10-6-10-5  M)-induced relaxations were compared in the control and ethanol groups.  Results: Blood ethanol levels in the control and ethanol-treated mice were 1.5 ± 0.3 mg/dL and 37.4 ± 4.1 mg/dL (P < 0.001), respectively.  Phenylephrine-induced contractile responses were potentiated in the ethanol group, with pD2 values of 4.92 ± 0.18 and 5.71 ± 0.21 (P <  0.01) in the corpus cavernosum obtained from control and alcohol-fed mice, respectively. The relaxant responses to acetylcholine and  EFS, but not Y-27632, were significantly augmented in the corpus cavernosum obtained from ethanol-treated mice. However, expression  and activation of Rho-kinase remained unchanged in the alcohol group.  Conclusion: Ethanol drinking may increase sensitivity to both vasoconstrictors and vasodilators in the mouse corpus cavernosum  without any alteration in Rho/Rho-kinase signalling.

Makale detayları

Rho/rho-kinase signalling in chronically alcohol-fed mice

Yazarlar: Havva KUBAT | Ahmet Sencer YURTSEVER | Kansu BÜYÜKAFŞAR

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2017 Vol.47 Issue 2, pp.668-674

Belge Tipi: Makale

Yayın Tarihi: 3/2017

Belge Dili: Türkçe

Document ID (Org No.): 2000751492

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Sayı

Yazarlar:

Havva KUBAT | Ahmet Sencer YURTSEVER | Kansu BÜYÜKAFŞAR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2017 Vol.47 Issue 2, pp.668-674

Belge Tipi:

Makale

Yayın Tarihi:

3/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000751492

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144