Metformin And Dichloroacetate Combination Exert A Synergistic Effect On Cell Viability Of Oral Squamous Cell Carcinoma

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doi:https://doi.org/10.32448/entupdates.569464

Makale detayları

Metformin And Dichloroacetate Combination Exert A Synergistic Effect On Cell Viability Of Oral Squamous Cell Carcinoma

Yazarlar:

Seniz INANC | Didem KELES | Gorkem ESKİİZMİR | Yasemin BASBİNAR | Gulgun OKTAY

Yayıncı:

Sürekli Eğitim ve Bilimsel Araştırmalar Derneği

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

ENT Updates 2019 Vol.9 Issue 2, pp.68-73

Belge Tipi:

Makale

Yayın Tarihi:

8/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000781627

Sıklık:

Üç Yıllık

eISSN:

2149-6498

pISSN:

2149-7109

DOI:

https://doi.org/10.32448/entupdates.569464

Özet:

 


Objective: To assess the effects of Metformin, Dichloroacetate (DCA) and their combination on cell viability in oral squamous cell carcinoma, UPCI-SCC-131 cell line.

Methods: UPCI-SCC-131 cells were plated in 96 E-plate (1x104 cells/well) and were treated with Metformin (1-16mM) and/or DCA (15-120mM) for 24-48-72h. xCELLigence SP system was used to monitor real time cell viability. In addition, drug combination index was analyzed with CompuSyn software according to Chou-Talalay method.

Results: Half-maximal inhibitory concentrations (IC50) of Metformin and DCA were found to be 3mM and 23mM, respectively, for 72 hours. CI values (0.76-0.80) in all combination groups below 1 indicated that Metformin/DCA combination had a moderate synergistic effect on cell viability in UPCI-SCC-131 cells.

Discussion: Metformin/DCA combination synergistically decreased the cell viability of UPCI-SCC-131 cells. Therefore, a combined application of Metformin and DCA may be considered as a candidate therapy for the “drug repositioning” of the treatment of oral cavity cancer.


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Makale detayları

Metformin And Dichloroacetate Combination Exert A Synergistic Effect On Cell Viability Of Oral Squamous Cell Carcinoma

Yazarlar: Seniz INANC | Didem KELES | Gorkem ESKİİZMİR | Yasemin BASBİNAR | Gulgun OKTAY

Yayıncı: Sürekli Eğitim ve Bilimsel Araştırmalar Derneği

Ana Başlıklar: Sağlık teşvikleri

Dergi: ENT Updates 2019 Vol.9 Issue 2, pp.68-73

Belge Tipi: Makale

Yayın Tarihi: 8/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000781627

Sıklık: Üç Yıllık

eISSN: 2149-6498

pISSN: 2149-7109

DOI: https://doi.org/10.32448/entupdates.569464

Sayı

Yazarlar:

Seniz INANC | Didem KELES | Gorkem ESKİİZMİR | Yasemin BASBİNAR | Gulgun OKTAY

Yayıncı:

Sürekli Eğitim ve Bilimsel Araştırmalar Derneği

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

ENT Updates 2019 Vol.9 Issue 2, pp.68-73

Belge Tipi:

Makale

Yayın Tarihi:

8/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000781627

Sıklık:

Üç Yıllık

eISSN:

2149-6498

pISSN:

2149-7109

DOI:

https://doi.org/10.32448/entupdates.569464