Non-Covalent Immobilization of Quince (Cydonia Oblonga) Polyphenol Oxidase

Hülya YAĞAR; Ayten SAĞIROĞLU

Makale detayları

Non-Covalent Immobilization of Quince (Cydonia Oblonga) Polyphenol Oxidase

Yazarlar:

Hülya YAĞAR | Ayten SAĞIROĞLU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2002 Vol.26 Issue 5, pp.751-758

Belge Tipi:

Makale

Yayın Tarihi:

4/2002

Belge Dili:

Türkçe

Document ID (Org No.):

2000729787

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527

Özet:

 

A partially purified polyphenol oxidase from quince (Cydonia  oblonga) was immobilized on bentonite by simple adsorption at pH 6.8.   The properties of the immobilized enzyme were compared to those of the  free enzyme.  Optimum pH and temperature were determined to be 9.0 and  45°C, respectively, showing the alteration of pH and  temperature profiles by immobilization. No drastic change was   observed in the Km  value after immobilization.  Catechol, L-DOPA,  p-cresole and pyrogallol were tested as substrates.  Thermal and  storage stability and reusability experiments were carried out.  It  was observed that the immobilized enzyme had storage stability for a  period of one year but had no reusability in the batch process.

 

A partially purified polyphenol oxidase from quince (Cydonia  oblonga) was immobilized on bentonite by simple adsorption at pH 6.8.   The properties of the immobilized enzyme were compared to those of the  free enzyme.  Optimum pH and temperature were determined to be 9.0 and  45°C, respectively, showing the alteration of pH and  temperature profiles by immobilization. No drastic change was   observed in the Km  value after immobilization.  Catechol, L-DOPA,  p-cresole and pyrogallol were tested as substrates.  Thermal and  storage stability and reusability experiments were carried out.  It  was observed that the immobilized enzyme had storage stability for a  period of one year but had no reusability in the batch process.

Makale detayları

Non-Covalent Immobilization of Quince (Cydonia Oblonga) Polyphenol Oxidase

Yazarlar: Hülya YAĞAR | Ayten SAĞIROĞLU

Yayıncı: TUBITAK

Dergi: Turkish Journal of Chemistry 2002 Vol.26 Issue 5, pp.751-758

Belge Tipi: Makale

Yayın Tarihi: 4/2002

Belge Dili: Türkçe

Document ID (Org No.): 2000729787

Sıklık: İki Aylık

eISSN: 1303-6130

pISSN: 1300-0527

Sayı

Yazarlar:

Hülya YAĞAR | Ayten SAĞIROĞLU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2002 Vol.26 Issue 5, pp.751-758

Belge Tipi:

Makale

Yayın Tarihi:

4/2002

Belge Dili:

Türkçe

Document ID (Org No.):

2000729787

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527