Analysis of the Cytotoxicity of  Four Dentin Bonding Agents on Gingival Fibroblasts

Jülide ÖZEN; Arzu ATAY; Fulya TOKSOY TOPÇU; Ali Uğur URAL

Makale detayları

Analysis of the Cytotoxicity of Four Dentin Bonding Agents on Gingival Fibroblasts

Yazarlar:

Jülide ÖZEN | Arzu ATAY | Fulya TOKSOY TOPÇU | Ali Uğur URAL

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2005 Vol.35 Issue 6, pp.395-399

Belge Tipi:

Makale

Yayın Tarihi:

5/2005

Belge Dili:

Türkçe

Document ID (Org No.):

2000756302

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

The objective of this study is to evaluate effects of four dentin bonding agents PromptL-Pop, Syntac, Pekabond, Scotchbond-1 on cell proliferation in human gingival fibroblast cultures. Under aseptic conditions, test specimens were placed in the centre of 24-well tissue culture trays.  Each well was covered at a concentration of 104 cells/cm2. The cultures were incubated at 37 ºC and cell proliferation was determined by the MTT method 24 h and 72 h after exposure. Statistical analysis was performed applying the Student t test. Statistical analysis of data showed that all materials caused noncytotoxic effects for 24 hours and cytoxic effects for 72 hours. Prompt L-pop displayed the highest number of cells whereas the lowest number of  cells  was found for Pekabond for 72 hours. These results support the proposal that some bonding agents may cause cytotoxic reactions under in vitro conditions.

 

The objective of this study is to evaluate effects of four dentin bonding agents PromptL-Pop, Syntac, Pekabond, Scotchbond-1 on cell proliferation in human gingival fibroblast cultures. Under aseptic conditions, test specimens were placed in the centre of 24-well tissue culture trays.  Each well was covered at a concentration of 104 cells/cm2. The cultures were incubated at 37 ºC and cell proliferation was determined by the MTT method 24 h and 72 h after exposure. Statistical analysis was performed applying the Student t test. Statistical analysis of data showed that all materials caused noncytotoxic effects for 24 hours and cytoxic effects for 72 hours. Prompt L-pop displayed the highest number of cells whereas the lowest number of  cells  was found for Pekabond for 72 hours. These results support the proposal that some bonding agents may cause cytotoxic reactions under in vitro conditions.

Makale detayları

Analysis of the Cytotoxicity of Four Dentin Bonding Agents on Gingival Fibroblasts

Yazarlar:

Jülide ÖZEN | Arzu ATAY | Fulya TOKSOY TOPÇU | Ali Uğur URAL

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2005 Vol.35 Issue 6, pp.395-399

Belge Tipi:

Makale

Yayın Tarihi:

5/2005

Belge Dili:

Türkçe

Document ID (Org No.):

2000756302

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Dentin bonding agents

gingival fibroblasts

MTT

cell culture

Sayı

Makale detayları

Analysis of the Cytotoxicity of Four Dentin Bonding Agents on Gingival Fibroblasts

Yazarlar: Jülide ÖZEN | Arzu ATAY | Fulya TOKSOY TOPÇU | Ali Uğur URAL

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2005 Vol.35 Issue 6, pp.395-399

Belge Tipi: Makale

Yayın Tarihi: 5/2005

Belge Dili: Türkçe

Document ID (Org No.): 2000756302

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Dentin bonding agents

gingival fibroblasts

MTT

cell culture

Sayı

Yazarlar:

Jülide ÖZEN | Arzu ATAY | Fulya TOKSOY TOPÇU | Ali Uğur URAL

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2005 Vol.35 Issue 6, pp.395-399

Belge Tipi:

Makale

Yayın Tarihi:

5/2005

Belge Dili:

Türkçe

Document ID (Org No.):

2000756302

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144