Investigation of the effects of tadalafil on the myocardium via electrical and isometric contractions and cardiac markers in Wistar rats

Duygun Altıntaş Aykan; Selma Yaman; Muhammed Seyithanoğlu; Ahmet Çağrı Aykan

Makale detayları

Investigation of the effects of tadalafil on the myocardium via electrical and isometric contractions and cardiac markers in Wistar rats

Yazarlar:

Duygun Altıntaş Aykan | Selma Yaman | Muhammed Seyithanoğlu | Ahmet Çağrı Aykan

Yayıncı:

Cukurova University

Ana Başlıklar:

Kalp ve Damar Cerrahisi

Dergi:

Cukurova Medical Journal Vol.49 Issue 3, pp.639-645

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001327853

pISSN:

2602-3032

Anahtar kelimeler:

Özet:

 Purpose: Tadalafil, a long-acting phosphodiesterase-5 PDE-5 inhibitor, is commonly used in the treatment of erectile dysfunction. This study investigates the contribution of tadalafil in cardiac function by measuring isometric and electrical contractions of myocardium in rats. Materials and Methods: Thirty rats were divided into five groups. Basal electrical contractions were recorded via electrocardiogram ECG for intervals PR ms, QRS ms, QT ms, Tp ms, Te ms, pathological Q and heart beatsmin. Then group 1 received saline for 7 days control; group 2 received tadalafil 1 mgkg; group 3 received tadalafil 10 mgkg; group 4 received tadalafil 1 mgkg for 7 days; group 5 received tadalafil 10 mgkg for 7 days. After treatments, electrical contractions were re-performed to analyze the differences in ECG. For isometric contractions, hearts were connected to isometric power transducer to determine myocardial contractile forces g, durations ms and frequencies Hz. Serum samples were collected for cardiac creatine kinase CK-MB and cardiac troponin I via ELISA. Results: We found a significant decrease in CK-MB in Group 2 395.56124.38 pgml and 3 377.8179.61 pgml, compared to Group 1 575.3283.54 pgml. The differences in cardiac contractile forces, contraction durations or frequencies were not statistically significant. Conclusion: Tadalafil did not exert obvious distruption on myocardial electrical or isometrical contractions. It is noteworthy that tadalafil 1 and 10 mgkg reduced serum CK-MB. Shortening in QT and decrease in heart rate may have important implications on myocardial functions.

 Purpose: Tadalafil, a long-acting phosphodiesterase-5 PDE-5 inhibitor, is commonly used in the treatment of erectile dysfunction. This study investigates the contribution of tadalafil in cardiac function by measuring isometric and electrical contractions of myocardium in rats. Materials and Methods: Thirty rats were divided into five groups. Basal electrical contractions were recorded via electrocardiogram ECG for intervals PR ms, QRS ms, QT ms, Tp ms, Te ms, pathological Q and heart beatsmin. Then group 1 received saline for 7 days control; group 2 received tadalafil 1 mgkg; group 3 received tadalafil 10 mgkg; group 4 received tadalafil 1 mgkg for 7 days; group 5 received tadalafil 10 mgkg for 7 days. After treatments, electrical contractions were re-performed to analyze the differences in ECG. For isometric contractions, hearts were connected to isometric power transducer to determine myocardial contractile forces g, durations ms and frequencies Hz. Serum samples were collected for cardiac creatine kinase CK-MB and cardiac troponin I via ELISA. Results: We found a significant decrease in CK-MB in Group 2 395.56124.38 pgml and 3 377.8179.61 pgml, compared to Group 1 575.3283.54 pgml. The differences in cardiac contractile forces, contraction durations or frequencies were not statistically significant. Conclusion: Tadalafil did not exert obvious distruption on myocardial electrical or isometrical contractions. It is noteworthy that tadalafil 1 and 10 mgkg reduced serum CK-MB. Shortening in QT and decrease in heart rate may have important implications on myocardial functions.

 Purpose: Tadalafil, a long-acting phosphodiesterase-5 PDE-5 inhibitor, is commonly used in the treatment of erectile dysfunction. This study investigates the contribution of tadalafil in cardiac function by measuring isometric and electrical contractions of myocardium in rats. Materials and Methods: Thirty rats were divided into five groups. Basal electrical contractions were recorded via electrocardiogram ECG for intervals PR ms, QRS ms, QT ms, Tp ms, Te ms, pathological Q and heart beatsmin. Then group 1 received saline for 7 days control; group 2 received tadalafil 1 mgkg; group 3 received tadalafil 10 mgkg; group 4 received tadalafil 1 mgkg for 7 days; group 5 received tadalafil 10 mgkg for 7 days. After treatments, electrical contractions were re-performed to analyze the differences in ECG. For isometric contractions, hearts were connected to isometric power transducer to determine myocardial contractile forces g, durations ms and frequencies Hz. Serum samples were collected for cardiac creatine kinase CK-MB and cardiac troponin I via ELISA. Results: We found a significant decrease in CK-MB in Group 2 395.56124.38 pgml and 3 377.8179.61 pgml, compared to Group 1 575.3283.54 pgml. The differences in cardiac contractile forces, contraction durations or frequencies were not statistically significant. Conclusion: Tadalafil did not exert obvious distruption on myocardial electrical or isometrical contractions. It is noteworthy that tadalafil 1 and 10 mgkg reduced serum CK-MB. Shortening in QT and decrease in heart rate may have important implications on myocardial functions.

 Amaç: Uzun etkili bir fosfodiesteraz-5 PDE-5 inhibitörü olan tadalafil, erektil disfonksiyon tedavisinde yaygın olarak kullanılmaktadır. Bu çalışma, sıçanlarda miyokardın izometrik ve elektriksel kasılmalarını ölçerek tadalafilin kalp fonksiyonuna katkısını araştırmaktadır. Gereç ve Yöntem: Otuz sıçan beş gruba ayrıldı. Bazal elektriksel kasılmalar elektrokardiyogram EKG aracılığıyla PR ms, QRS ms, QT ms, Tp ms, Te ms, patolojik Q ve kalp atımdakika aralıklarında kaydedildi. Daha sonra grup 1e 7 gün boyunca salin verildi kontrol; grup 2ye 1 mgkg tadalafil verildi; grup 3e 10 mgkg tadalafil verildi; grup 4e 7 gün süreyle 1 mgkg tadalafil verildi; grup 5e 7 gün süreyle 10 mgkg tadalafil verildi. Tedavilerden sonra EKGdeki farklılıkları analiz etmek için elektriksel kasılmalar yeniden gerçekleştirildi. İzometrik kasılmalar için kalpler, miyokardiyal kasılma kuvvetlerini g, sürelerini ms ve frekanslarını Hz belirlemek için izometrik güç dönüştürücüye bağlandı. ELISA yoluyla kardiyak kreatin kinaz CK-MB ve kardiyak troponin I için serum örnekleri toplandı. Bulgular: CK-MB değerlerinde Grup 2de 395,56124,38 pgml ve Grup 3te 377,8179,61 pgml, Grup 1e 575,3283,54 pgml göre anlamlı bir azalma bulduk. Kardiyak kasılma kuvvetleri, kasılma süreleri veya frekanslarındaki farklılıklar istatistiksel olarak anlamlı değildi. Sonuç: Tadalafil, miyokardın elektriksel veya izometrik kasılmaları üzerinde belirgin bir bozulma yaratmadı. Tadalafil 1 ve 10 mgkgın serum CK-MByi azalttığı dikkat çekicidir. QTdeki kısalma ve kalp hızındaki azalmanın miyokardiyal fonksiyonlar üzerinde önemli etkileri olabilir.

Makale detayları

Investigation of the effects of tadalafil on the myocardium via electrical and isometric contractions and cardiac markers in Wistar rats

Yazarlar:

Duygun Altıntaş Aykan | Selma Yaman | Muhammed Seyithanoğlu | Ahmet Çağrı Aykan

Yayıncı:

Cukurova University

Ana Başlıklar:

Kalp ve Damar Cerrahisi

Dergi:

Cukurova Medical Journal Vol.49 Issue 3, pp.639-645

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001327853

pISSN:

2602-3032

Tadalafil

Muscle contraction

Myocardium

ElectrocardiographyTadalafil

Kas kontraksiyonu

Miyokard

Elektrokardiyografi

Sayı

Makale detayları

Yazarlar: Duygun Altıntaş Aykan | Selma Yaman | Muhammed Seyithanoğlu | Ahmet Çağrı Aykan

Yayıncı: Cukurova University

Ana Başlıklar: Kalp ve Damar Cerrahisi

Dergi: Cukurova Medical Journal Vol.49 Issue 3, pp.639-645

Belge Tipi: Makale

Yayın Tarihi: 9/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001327853

pISSN: 2602-3032

Sayı

Yazarlar:

Duygun Altıntaş Aykan | Selma Yaman | Muhammed Seyithanoğlu | Ahmet Çağrı Aykan

Yayıncı:

Cukurova University

Ana Başlıklar:

Kalp ve Damar Cerrahisi

Dergi:

Cukurova Medical Journal Vol.49 Issue 3, pp.639-645

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001327853

pISSN:

2602-3032