The effect of kisspeptin on spermatogenesis and apoptosis in rats

Nilüfer AYTÜRK; Tülin FIRAT; Aysel KÜKNER; Candan ÖZOĞUL; Fatma TÖRE; İsmail Engin KANDIRALI; Bayram YILMAZ

Makale detayları

The effect of kisspeptin on spermatogenesis and apoptosis in rats

Yazarlar:

Nilüfer AYTÜRK | Tülin FIRAT | Aysel KÜKNER | Candan ÖZOĞUL | Fatma TÖRE | İsmail Engin KANDIRALI | Bayram YILMAZ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2017 Vol.47 Issue 1, pp.334-342

Belge Tipi:

Makale

Yayın Tarihi:

1/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000751631

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Özet:

 

Background/aim: To study the effect of kisspeptin, a gonadotropin release stimulator, on the testicular tissue of the rat.   Materials and methods: Four groups were formed as follows: control, Kiss-10 50 nmol administration for 1 day, Kiss-10 administration for 13 days, and one last group kept for 7 days following Kiss-10 applied for 13 days. Testicular tissues were stained with hematoxylin-eosin, periodic acid Schiff, Masson trichrome staining, terminal deoxynucleotidyl transferased UTP nick-end labeling, and Ki-67 immune staining. Serum testosterone levels were determined.  Results: Serum testosterone level increased following acute application, while it was reduced by chronic treatment. Spermatogenic cells as stained by Ki-67 and TUNEL increased in the treated groups compared to the controls. Following a 7-day rest after treatment, a decrease in testosterone levels and Ki-67-stained cell numbers and an increase in TUNEL-stained cells were observed. Leydig cells showed increased vacuolization in the Kiss-1 group. Leydig cell vacuolization continued in the Kiss (13) group and was reduced in the Kiss (13 + 7) group.   Conclusion: Kiss-10 increased spermatogenic cell proliferation, while testosterone level and proliferation decreased and apoptosis increased during the waiting period.

Makale detayları

The effect of kisspeptin on spermatogenesis and apoptosis in rats

Yazarlar: Nilüfer AYTÜRK | Tülin FIRAT | Aysel KÜKNER | Candan ÖZOĞUL | Fatma TÖRE | İsmail Engin KANDIRALI | Bayram YILMAZ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2017 Vol.47 Issue 1, pp.334-342

Belge Tipi: Makale

Yayın Tarihi: 1/2017

Belge Dili: Türkçe

Document ID (Org No.): 2000751631

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Sayı

Yazarlar:

Nilüfer AYTÜRK | Tülin FIRAT | Aysel KÜKNER | Candan ÖZOĞUL | Fatma TÖRE | İsmail Engin KANDIRALI | Bayram YILMAZ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2017 Vol.47 Issue 1, pp.334-342

Belge Tipi:

Makale

Yayın Tarihi:

1/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000751631

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144