In vitro mutagenesis of Etlingera elatior (Jack) and early detection of mutation using RAPD markers

Muhamad Fahmi YUNUS; Maheran Abd AZIZ; Mihdzar Abdul KADIR; Siti Khalijah DAUD; Azmi Abdul RASHID

Makale detayları

In vitro mutagenesis of Etlingera elatior (Jack) and early detection of mutation using RAPD markers

Yazarlar:

Muhamad Fahmi YUNUS | Maheran Abd AZIZ | Mihdzar Abdul KADIR | Siti Khalijah DAUD | Azmi Abdul RASHID

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 6, pp.716-725

Belge Tipi:

Makale

Yayın Tarihi:

5/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000812336

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Mutation breeding techniques in combination with tissue culture and molecular marker methods provide a powerful tool for improvement of vegetatively propagated plants. The aim of this study was to develop a protocol for shoot regeneration and mutation induction of Etlingera elatior. The results of irradiation on in vitro buds of E. elatior showed LD50 to be 10 Gy, with the survival of explants being sharply reduced at this dosage. All 8 selected gamma irradiated regenerants were differentiated from the untreated control based on the banding patterns obtained using 9 primers, which generated 59 reproducible bands, whereby 35 (55.31%) were found to be polymorphic. Jaccard's coefficient of similarity values ranging from 0.537 to 0.860 were indicative of the level of genetic variation among the mutants studied. For comparison between the potential lines (PL) and the control, a maximum similarity value (0.814) was observed in PL1 mutant, while the minimum value (0.537) was observed in PL7. In summary, a combination of irradiation, regeneration, multiplication, and random amplification of polymorphic DNA (RAPD) analysis for early screening of mutants can speed up the breeding program of E. elatior.

 

Mutation breeding techniques in combination with tissue culture and molecular marker methods provide a powerful tool for improvement of vegetatively propagated plants. The aim of this study was to develop a protocol for shoot regeneration and mutation induction of Etlingera elatior. The results of irradiation on in vitro buds of E. elatior showed LD50 to be 10 Gy, with the survival of explants being sharply reduced at this dosage. All 8 selected gamma irradiated regenerants were differentiated from the untreated control based on the banding patterns obtained using 9 primers, which generated 59 reproducible bands, whereby 35 (55.31%) were found to be polymorphic. Jaccard's coefficient of similarity values ranging from 0.537 to 0.860 were indicative of the level of genetic variation among the mutants studied. For comparison between the potential lines (PL) and the control, a maximum similarity value (0.814) was observed in PL1 mutant, while the minimum value (0.537) was observed in PL7. In summary, a combination of irradiation, regeneration, multiplication, and random amplification of polymorphic DNA (RAPD) analysis for early screening of mutants can speed up the breeding program of E. elatior.

Makale detayları

In vitro mutagenesis of Etlingera elatior (Jack) and early detection of mutation using RAPD markers

Yazarlar:

Muhamad Fahmi YUNUS | Maheran Abd AZIZ | Mihdzar Abdul KADIR | Siti Khalijah DAUD | Azmi Abdul RASHID

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 6, pp.716-725

Belge Tipi:

Makale

Yayın Tarihi:

5/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000812336

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Key words: Etlingera elatior

LD50

genetic variation

mutation

potential line

RAPD

Sayı

Makale detayları

In vitro mutagenesis of Etlingera elatior (Jack) and early detection of mutation using RAPD markers

Yazarlar: Muhamad Fahmi YUNUS | Maheran Abd AZIZ | Mihdzar Abdul KADIR | Siti Khalijah DAUD | Azmi Abdul RASHID

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2013 Vol.37 Issue 6, pp.716-725

Belge Tipi: Makale

Yayın Tarihi: 5/2013

Belge Dili: Türkçe

Document ID (Org No.): 2000812336

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Key words: Etlingera elatior

LD50

genetic variation

mutation

potential line

RAPD

Sayı

Yazarlar:

Muhamad Fahmi YUNUS | Maheran Abd AZIZ | Mihdzar Abdul KADIR | Siti Khalijah DAUD | Azmi Abdul RASHID

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 6, pp.716-725

Belge Tipi:

Makale

Yayın Tarihi:

5/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000812336

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152